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Literature summary extracted from

  • Johansson, H.E.; Johansson, M.K.; Wong, A.C.; Armstrong, E.S.; Peterson, E.J.; Grant, R.E.; Roy, M.A.; Reddington, M.V.; Cook, R.M.
    BTI1, an azoreductase with pH-dependent substrate specificity (2011), Appl. Environ. Microbiol., 77, 4223-4225.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.6 0.0097
-
 o-methyl red pH not specified in the publication, 22°C Staphylococcus aureus
1.7.1.6 0.059
-
 BHQ-10 pH not specified in the publication, 22°C Staphylococcus aureus
1.7.1.6 0.3
-
 Methyl orange pH not specified in the publication, 22°C Staphylococcus aureus
1.7.1.6 0.51
-
 flame orange pH not specified in the publication, 22°C Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.6 Staphylococcus aureus Q50H63
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.6 BHQ-10 + NADPH + H+
-
 Staphylococcus aureus ? + NADP+
-
 ?
1.7.1.6 flame orange + NADPH + H+
-
 Staphylococcus aureus ? + NADP+
-
 ?
1.7.1.6 Janus green B + NADPH + H+
-
 Staphylococcus aureus ? + NADP+
-
 ?
1.7.1.6 methyl orange + NADPH + H+
-
 Staphylococcus aureus ? + NADP+
-
 ?
1.7.1.6 additional information ammoniumazo-1, citrus yellow, orange G, Ponceau BS, and ruby red are inert to reduction with BTI1 Staphylococcus aureus ?
-
 ?
1.7.1.6 o-methyl red + NADPH + H+
-
 Staphylococcus aureus ? + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.1.6 AzoR
-
 Staphylococcus aureus
1.7.1.6 BTI1
-
 Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.6 additional information
-
 the reduction rate is both dye and pH specific Staphylococcus aureus
1.7.1.6 5.5 6 substrate Janus green B Staphylococcus aureus
1.7.1.6 6 6.5 substrates BHQ-10 and o-methyl red Staphylococcus aureus
1.7.1.6 6
-
 substrate methyl orange Staphylococcus aureus
1.7.1.6 8.3
-
 flame orange reduction rate increases up to at least pH 8.3 Staphylococcus aureus