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Literature summary extracted from

  • Harreither, W.; Sygmund, C.; Augustin, M.; Narciso, M.; Rabinovich, M.L.; Gorton, L.; Haltrich, D.; Ludwig, R.
    Catalytic properties and classification of cellobiose dehydrogenases from Ascomycetes (2011), Appl. Environ. Microbiol., 77, 1804-1815.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.18 0.072
-
 cellobiose at pH 6.0 and 30°C Amesia atrobrunnea
1.1.99.18 0.125
-
 cellobiose at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 0.14
-
 cellobiose at pH 5.0 and 30°C Thermothelomyces fergusii
1.1.99.18 0.16
-
 cellobiose at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 0.16
-
 cellobiose at pH 5.5 and 30°C Neurospora crassa
1.1.99.18 0.17
-
 cellobiose at pH 5.5 and 30°C Stachybotrys bisbyi
1.1.99.18 0.185
-
 lactose at pH 5.5 and 30°C Stachybotrys bisbyi
1.1.99.18 0.2
-
 lactose at pH 5.0 and 30°C Thermothelomyces fergusii
1.1.99.18 0.215
-
 lactose at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 0.217
-
 lactose at pH 6.0 and 30°C Amesia atrobrunnea
1.1.99.18 0.23
-
 lactose at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 0.325
-
 lactose at pH 5.5 and 30°C Neurospora crassa
1.1.99.18 2.2
-
 maltose at pH 5.0 and 30°C Thermothelomyces fergusii
1.1.99.18 8
-
 maltose at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 15.5
-
 maltose at pH 6.0 and 30°C Amesia atrobrunnea
1.1.99.18 18
-
 maltose at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 50
-
 maltose at pH 5.5 and 30°C Stachybotrys bisbyi
1.1.99.18 71
-
 D-glucose at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 79
-
 D-glucose at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 87
-
 D-glucose at pH 6.0 and 30°C Amesia atrobrunnea
1.1.99.18 120
-
 D-glucose at pH 5.0 and 30°C Thermothelomyces fergusii
1.1.99.18 120
-
 maltose at pH 5.5 and 30°C Neurospora crassa
1.1.99.18 4000
-
 D-glucose at pH 5.´5 and 30°C Neurospora crassa
1.1.99.18 17000
-
 D-glucose at pH 5.´5 and 30°C Stachybotrys bisbyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.18 81840
-
 x * 81840, calculated from amino acid sequence Thermothelomyces fergusii
1.1.99.18 83587
-
 x * 83587, calculated from amino acid sequence Neurospora crassa
1.1.99.18 83901
-
 x * 83901, calculated from amino acid sequence Hypoxylon haematostroma
1.1.99.18 85000
-
 x * 85000, SDS-PAGE Thermothelomyces fergusii
1.1.99.18 85000
-
 x * 85000, SDS-PAGE Hypoxylon haematostroma
1.1.99.18 85942
-
 x * 85942, calculated from amino acid sequence Amesia atrobrunnea
1.1.99.18 86688
-
 x * 86688, calculated from amino acid sequence Stachybotrys bisbyi
1.1.99.18 86721
-
 x * 86721, calculated from amino acid sequence Dichomera saubinetii
1.1.99.18 90000
-
 x * 90000, SDS-PAGE Neurospora crassa
1.1.99.18 90000
-
 x * 90000, SDS-PAGE Amesia atrobrunnea
1.1.99.18 100000
-
 x * 100000, SDS-PAGE Stachybotrys bisbyi
1.1.99.18 115000
-
 x * 115000, SDS-PAGE Dichomera saubinetii

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.18 Amesia atrobrunnea E7D6C0
-
-
1.1.99.18 Dichomera saubinetii E7D6C1
-
-
1.1.99.18 Hypoxylon haematostroma
-
-
-
1.1.99.18 Neurospora crassa
-
-
-
1.1.99.18 Stachybotrys bisbyi E7D6C4
-
-
1.1.99.18 Thermothelomyces fergusii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.18 DEAE-Sepharose column chromatography and phenyl-Source column chromatography Neurospora crassa
1.1.99.18 DEAE-Sepharose column chromatography and phenyl-Source column chromatography Thermothelomyces fergusii
1.1.99.18 DEAE-Sepharose column chromatography and phenyl-Source column chromatography Stachybotrys bisbyi
1.1.99.18 DEAE-Sepharose column chromatography and phenyl-Source column chromatography Hypoxylon haematostroma
1.1.99.18 DEAE-Sepharose column chromatography and phenyl-Source column chromatography Amesia atrobrunnea
1.1.99.18 DEAE-Sepharose column chromatography and phenyl-Source column chromatography Dichomera saubinetii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.18 2.2
-
 after 14.8fold purification, at pH 4.0 and 37°C Stachybotrys bisbyi
1.1.99.18 2.7
-
 after 14.8fold purification, at pH 4.5 and 37°C Stachybotrys bisbyi
1.1.99.18 5.7
-
 after 48.1fold purification, at pH 4.0 and 37°C Neurospora crassa
1.1.99.18 7.1
-
 after 293fold purification, at pH 4.0 and 37°C Amesia atrobrunnea
1.1.99.18 7.4
-
 after 21.2fold purification, at pH 4.0 and 37°C Hypoxylon haematostroma
1.1.99.18 8.9
-
 after 7.3fold purification, at pH 4.0 and 37°C Thermothelomyces fergusii
1.1.99.18 9
-
 after 21.2fold purification, at pH 5.0 and 37°C Hypoxylon haematostroma
1.1.99.18 10.3
-
 after 48.1fold purification, at pH 5.5 and 37°C Neurospora crassa
1.1.99.18 11.5
-
 after 293fold purification, at pH 6.0 and 37°C Amesia atrobrunnea
1.1.99.18 12.3
-
 after 26.3fold purification, at pH 4.0 and 37°C Dichomera saubinetii
1.1.99.18 13.2
-
 after 26.3fold purification, at pH 5.5 and 37°C Dichomera saubinetii
1.1.99.18 17.8
-
 after 7.3fold purification, at pH 5.0 and 37°C Thermothelomyces fergusii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.18 cellobiose + 2,6-dichloroindophenol
-
 Neurospora crassa cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
-
 ?
1.1.99.18 cellobiose + 2,6-dichloroindophenol
-
 Thermothelomyces fergusii cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
-
 ?
1.1.99.18 cellobiose + 2,6-dichloroindophenol
-
 Stachybotrys bisbyi cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
-
 ?
1.1.99.18 cellobiose + 2,6-dichloroindophenol
-
 Hypoxylon haematostroma cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
-
 ?
1.1.99.18 cellobiose + 2,6-dichloroindophenol
-
 Amesia atrobrunnea cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
-
 ?
1.1.99.18 cellobiose + 2,6-dichloroindophenol
-
 Dichomera saubinetii cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
-
 ?
1.1.99.18 D-glucose + 2,6-dichloroindophenol
-
 Neurospora crassa D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
-
 ?
1.1.99.18 D-glucose + 2,6-dichloroindophenol
-
 Thermothelomyces fergusii D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
-
 ?
1.1.99.18 D-glucose + 2,6-dichloroindophenol
-
 Hypoxylon haematostroma D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
-
 ?
1.1.99.18 D-glucose + 2,6-dichloroindophenol
-
 Amesia atrobrunnea D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
-
 ?
1.1.99.18 D-glucose + 2,6-dichloroindophenol
-
 Dichomera saubinetii D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
-
 ?
1.1.99.18 D-glucose + oxidized 2,6-dichlorophenolindophenol
-
 Stachybotrys bisbyi D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.18 lactose + 2,6-dichloroindophenol
-
 Neurospora crassa 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
-
 ?
1.1.99.18 lactose + 2,6-dichloroindophenol
-
 Thermothelomyces fergusii 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
-
 ?
1.1.99.18 lactose + 2,6-dichloroindophenol
-
 Stachybotrys bisbyi 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
-
 ?
1.1.99.18 lactose + 2,6-dichloroindophenol
-
 Amesia atrobrunnea 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
-
 ?
1.1.99.18 lactose + 2,6-dichloroindophenol
-
 Dichomera saubinetii 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
-
 ?
1.1.99.18 lactose + 2,6-dichloroindophenol
-
 Hypoxylon haematostroma 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6dichloro-indophenol
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Neurospora crassa 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Thermothelomyces fergusii 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Stachybotrys bisbyi 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Hypoxylon haematostroma 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Amesia atrobrunnea 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 lactose + cytochrome c
-
 Dichomera saubinetii 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
1.1.99.18 maltose + oxidized 2,6-dichlorophenolindophenol
-
 Neurospora crassa 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.18 maltose + oxidized 2,6-dichlorophenolindophenol
-
 Thermothelomyces fergusii 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.18 maltose + oxidized 2,6-dichlorophenolindophenol
-
 Stachybotrys bisbyi 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.18 maltose + oxidized 2,6-dichlorophenolindophenol
-
 Hypoxylon haematostroma 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.18 maltose + oxidized 2,6-dichlorophenolindophenol
-
 Amesia atrobrunnea 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.18 maltose + oxidized 2,6-dichlorophenolindophenol
-
 Dichomera saubinetii 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.18 ? x * 90000, SDS-PAGE Neurospora crassa
1.1.99.18 ? x * 90000, SDS-PAGE Amesia atrobrunnea
1.1.99.18 ? x * 100000, SDS-PAGE Stachybotrys bisbyi
1.1.99.18 ? x * 115000, SDS-PAGE Dichomera saubinetii
1.1.99.18 ? x * 85000, SDS-PAGE Thermothelomyces fergusii
1.1.99.18 ? x * 85000, SDS-PAGE Hypoxylon haematostroma
1.1.99.18 ? x * 81840, calculated from amino acid sequence Thermothelomyces fergusii
1.1.99.18 ? x * 83587, calculated from amino acid sequence Neurospora crassa
1.1.99.18 ? x * 83901, calculated from amino acid sequence Hypoxylon haematostroma
1.1.99.18 ? x * 85942, calculated from amino acid sequence Amesia atrobrunnea
1.1.99.18 ? x * 86688, calculated from amino acid sequence Stachybotrys bisbyi
1.1.99.18 ? x * 86721, calculated from amino acid sequence Dichomera saubinetii

Synonyms

EC Number Synonyms Comment Organism
1.1.99.18 Cdh
-
 Neurospora crassa
1.1.99.18 Cdh
-
 Thermothelomyces fergusii
1.1.99.18 Cdh
-
 Stachybotrys bisbyi
1.1.99.18 Cdh
-
 Hypoxylon haematostroma
1.1.99.18 Cdh
-
 Amesia atrobrunnea
1.1.99.18 Cdh
-
 Dichomera saubinetii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.18 0.51
-
 maltose at pH 5.5 and 30°C Stachybotrys bisbyi
1.1.99.18 0.6
-
 maltose at pH 5.5 and 30°C Neurospora crassa
1.1.99.18 1.38
-
 maltose at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 1.45
-
 maltose at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 1.8
-
 maltose at pH 5.0 and 30°C Thermothelomyces fergusii
1.1.99.18 4
-
 maltose at pH 6.0 and 30°C Amesia atrobrunnea
1.1.99.18 13
-
 cellobiose at pH 5.0 and 30°C Thermothelomyces fergusii
1.1.99.18 16.8
-
 D-glucose at pH 6.0 and 30°C Amesia atrobrunnea
1.1.99.18 18.4
-
 D-glucose at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 19.9
-
 cellobiose at pH 5.5 and 30°C Stachybotrys bisbyi
1.1.99.18 20.1
-
 cellobiose at pH 6.0 and 30°C Amesia atrobrunnea
1.1.99.18 20.2
-
 lactose at pH 5.5 and 30°C Stachybotrys bisbyi
1.1.99.18 21.8
-
 cellobiose at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 23.6
-
 D-glucose at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 24.2
-
 D-glucose at pH 5.5 and 30°C Stachybotrys bisbyi
1.1.99.18 24.7
-
 lactose at pH 5.0 and 30°C Thermothelomyces fergusii
1.1.99.18 25
-
 lactose at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 25.6
-
 D-glucose at pH 5.0 and 30°C Thermothelomyces fergusii
1.1.99.18 27.8
-
 cellobiose at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 28.9
-
 lactose at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 33.7
-
 lactose at pH 6.0 and 30°C Amesia atrobrunnea
1.1.99.18 37.7
-
 D-glucose at pH 5.5 and 30°C Neurospora crassa
1.1.99.18 51.8
-
 lactose at pH 5.5 and 30°C Neurospora crassa
1.1.99.18 53.1
-
 cellobiose at pH 5.5 and 30°C Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.18 4.5
-
-
 Stachybotrys bisbyi
1.1.99.18 5
-
-
 Thermothelomyces fergusii
1.1.99.18 5
-
-
 Hypoxylon haematostroma
1.1.99.18 5
-
-
 Dichomera saubinetii
1.1.99.18 5.5
-
-
 Neurospora crassa
1.1.99.18 6
-
-
 Amesia atrobrunnea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.99.18 Thermothelomyces fergusii isoelectric focusing
-
 3.8
1.1.99.18 Amesia atrobrunnea isoelectric focusing
-
 4.1
1.1.99.18 Dichomera saubinetii isoelectric focusing
-
 4.2
1.1.99.18 Stachybotrys bisbyi isoelectric focusing
-
 4.3
1.1.99.18 Hypoxylon haematostroma isoelectric focusing
-
 4.3
1.1.99.18 Neurospora crassa isoelectric focusing
-
 4.4
1.1.99.18 Thermothelomyces fergusii calculated from amino acid sequence
-
 4.6
1.1.99.18 Amesia atrobrunnea calculated from amino acid sequence
-
 5
1.1.99.18 Dichomera saubinetii calculated from amino acid sequence
-
 5
1.1.99.18 Stachybotrys bisbyi calculated from amino acid sequence
-
 5.1
1.1.99.18 Hypoxylon haematostroma calculated from amino acid sequence
-
 5.7
1.1.99.18 Neurospora crassa calculated from amino acid sequence
-
 6.7

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.99.18 0.005
-
 maltose at pH 5.5 and 30°C Neurospora crassa
1.1.99.18 0.009
-
 D-glucose at pH 5.5 and 30°C Neurospora crassa
1.1.99.18 0.01
-
 maltose at pH 5.5 and 30°C Stachybotrys bisbyi
1.1.99.18 0.014
-
 D-glucose at pH 5.5 and 30°C Stachybotrys bisbyi
1.1.99.18 0.08
-
 maltose at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 0.175
-
 maltose at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 0.195
-
 D-glucose at pH 6.0 and 30°C Amesia atrobrunnea
1.1.99.18 0.215
-
 D-glucose at pH 5.0 and 30°C Thermothelomyces fergusii
1.1.99.18 0.26
-
 maltose at pH 6.0 and 30°C Amesia atrobrunnea
1.1.99.18 0.26
-
 D-glucose at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 0.3
-
 D-glucose at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 0.82
-
 maltose at pH 5.0 and 30°C Thermothelomyces fergusii
1.1.99.18 93
-
 cellobiose at pH 5.0 and 30°C Thermothelomyces fergusii
1.1.99.18 109
-
 lactose at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 109
-
 lactose at pH 5.5 and 30°C Stachybotrys bisbyi
1.1.99.18 117
-
 cellobiose at pH 5.5 and 30°C Stachybotrys bisbyi
1.1.99.18 124
-
 lactose at pH 5.0 and 30°C Thermothelomyces fergusii
1.1.99.18 134
-
 lactose at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 136
-
 cellobiose at pH 5.0 and 30°C Hypoxylon haematostroma
1.1.99.18 155
-
 lactose at pH 6.0 and 30°C Amesia atrobrunnea
1.1.99.18 159
-
 lactose at pH 5.5 and 30°C Neurospora crassa
1.1.99.18 222
-
 cellobiose at pH 5.0 and 30°C Dichomera saubinetii
1.1.99.18 279
-
 cellobiose at pH 6.0 and 30°C Amesia atrobrunnea
1.1.99.18 332
-
 cellobiose at pH 5.5 and 30°C Neurospora crassa