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Literature summary extracted from

  • Ng, F.S.; Wright, D.M.; Seah, S.Y.
    Characterization of a phosphotriesterase-like lactonase from Sulfolobus solfataricus and its immobilization for disruption of quorum sensing (2010), Appl. Environ. Microbiol., 77, 1181-1186.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.81 overexpressed in Pseudomonas putida KT2440 Saccharolobus solfataricus
3.1.8.1 overexpressed in Pseudomonas putida KT2440 Saccharolobus solfataricus

General Stability

EC Number General Stability Organism
3.1.1.81 when the enzyme is immobilized on nanoalumina membranes 25% of the activity is retained upon immobilization Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.81 0.05
-
 N-(3-oxodecanoyl)-L-homoserine lactone pH 8.0, 25°C Saccharolobus solfataricus
3.1.1.81 0.16
-
 N-(3-oxooctanoyl)-L-homoserine lactone pH 8.0, 25°C Saccharolobus solfataricus
3.1.1.81 0.17
-
 N-(3-oxododecanoyl)-L-homoserine lactone pH 8.0, 25°C Saccharolobus solfataricus
3.1.1.81 5.6
-
 N-(3-oxohexanoyl)-L-homoserine lactone pH 8.0, 25°C Saccharolobus solfataricus
3.1.8.1 2.36
-
 paraoxon pH 8.0, 70°C Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.81 Co2+ 2.2 Co2+ per active site in the enzyme that is purified from recombinant Pseudomonas putida Saccharolobus solfataricus
3.1.8.1 Co2+ 2.2 Co2+ per active site in the enzyme that is purified from recombinant Pseudomonas putida Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.81 34000
-
 x * 34000, SDS-PAGE Saccharolobus solfataricus
3.1.8.1 34000
-
 x * 34000, SDS-PAGE Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.81 Saccharolobus solfataricus Q97VT7
-
-
3.1.1.81 Saccharolobus solfataricus P2 Q97VT7
-
-
3.1.8.1 Saccharolobus solfataricus Q97VT7
-
-
3.1.8.1 Saccharolobus solfataricus P2 Q97VT7
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.1.8.1 original color intensity after 23 days of storage in water or 20 mM sodium HEPES buffer (pH 8.0) Saccharolobus solfataricus
3.1.8.1 storage under dry conditions is less favorable for the stability of the enzyme, as the amount of 4-nitrophenolate produced from 1 mM paraoxon is reduced to 60 to 75% of the initial amount after 23 days Saccharolobus solfataricus
3.1.8.1 when the enzyme is immobilized on nanoalumina membranes 25% of the activity is retained upon immobilization. When tested with 1 mM paraoxon, the membranes produced at least 90% of their Saccharolobus solfataricus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.81
-
 Saccharolobus solfataricus
3.1.8.1
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.81 N-(3-oxodecanoyl)-L-homoserine lactone + H2O the enzyme has a preference for N-acyl homoserine lactones with acyl chain lengths of at least 8 carbon atoms Saccharolobus solfataricus N-(3-oxodecanoyl)-L-homoserine
-
 ?
3.1.1.81 N-(3-oxodecanoyl)-L-homoserine lactone + H2O the enzyme has a preference for N-acyl homoserine lactones with acyl chain lengths of at least 8 carbon atoms Saccharolobus solfataricus P2 N-(3-oxodecanoyl)-L-homoserine
-
 ?
3.1.1.81 N-(3-oxododecanoyl)-L-homoserine lactone + H2O the enzyme has a preference for N-acyl homoserine lactones with acyl chain lengths of at least 8 carbon atoms Saccharolobus solfataricus N-(3-oxododecanoyl)-L-homoserine
-
 ?
3.1.1.81 N-(3-oxododecanoyl)-L-homoserine lactone + H2O the enzyme has a preference for N-acyl homoserine lactones with acyl chain lengths of at least 8 carbon atoms Saccharolobus solfataricus P2 N-(3-oxododecanoyl)-L-homoserine
-
 ?
3.1.1.81 N-(3-oxohexanoyl)-L-homoserine lactone + H2O the enzyme has a preference for N-acyl homoserine lactones with acyl chain lengths of at least 8 carbon atoms Saccharolobus solfataricus N-(3-oxohexanoyl)-L-homoserine
-
 ?
3.1.1.81 N-(3-oxohexanoyl)-L-homoserine lactone + H2O the enzyme has a preference for N-acyl homoserine lactones with acyl chain lengths of at least 8 carbon atoms Saccharolobus solfataricus P2 N-(3-oxohexanoyl)-L-homoserine
-
 ?
3.1.1.81 N-(3-oxooctanoyl)-L-homoserine lactone + H2O the enzyme has a preference for N-acyl homoserine lactones with acyl chain lengths of at least 8 carbon atoms Saccharolobus solfataricus N-(3-oxooctanoyl)-L-homoserine
-
 ?
3.1.1.81 N-(3-oxooctanoyl)-L-homoserine lactone + H2O the enzyme has a preference for N-acyl homoserine lactones with acyl chain lengths of at least 8 carbon atoms Saccharolobus solfataricus P2 N-(3-oxooctanoyl)-L-homoserine
-
 ?
3.1.8.1 paraoxon + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + diethyl phosphate
-
 ?
3.1.8.1 paraoxon + H2O
-
 Saccharolobus solfataricus P2 4-nitrophenol + diethyl phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.81 ? x * 34000, SDS-PAGE Saccharolobus solfataricus
3.1.8.1 ? x * 34000, SDS-PAGE Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.81 N-acyl homoserine lactonase
-
 Saccharolobus solfataricus
3.1.1.81 SSO2522 locus name Saccharolobus solfataricus
3.1.1.81 SsoPox bifunctional enzyme with organophosphate hydrolase and N-acyl homoserine lactonase activities Saccharolobus solfataricus
3.1.8.1 organophosphate hydrolase
-
 Saccharolobus solfataricus
3.1.8.1 paraoxonase
-
 Saccharolobus solfataricus
3.1.8.1 SSO2522 locus name Saccharolobus solfataricus
3.1.8.1 SsoPox bifunctional enzyme with organophosphate hydrolase and N-acyl homoserine lactonase activities Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.81 0.52
-
 N-(3-oxohexanoyl)-L-homoserine lactone pH 8.0, 25°C Saccharolobus solfataricus
3.1.1.81 0.95
-
 N-(3-oxododecanoyl)-L-homoserine lactone pH 8.0, 25°C Saccharolobus solfataricus
3.1.1.81 1
-
 N-(3-oxooctanoyl)-L-homoserine lactone pH 8.0, 25°C Saccharolobus solfataricus
3.1.1.81 1.5
-
 N-(3-oxodecanoyl)-L-homoserine lactone pH 8.0, 25°C Saccharolobus solfataricus
3.1.8.1 2.29
-
 paraoxon pH 8.0, 70°C Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.81 0.093
-
 N-(3-oxohexanoyl)-L-homoserine lactone pH 8.0, 25°C Saccharolobus solfataricus
3.1.1.81 5.5
-
 N-(3-oxododecanoyl)-L-homoserine lactone pH 8.0, 25°C Saccharolobus solfataricus
3.1.1.81 6.6
-
 N-(3-oxooctanoyl)-L-homoserine lactone pH 8.0, 25°C Saccharolobus solfataricus
3.1.1.81 290
-
 N-(3-oxodecanoyl)-L-homoserine lactone pH 8.0, 25°C Saccharolobus solfataricus
3.1.8.1 0.97
-
 paraoxon pH 8.0, 70°C Saccharolobus solfataricus