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Literature summary extracted from

  • Ando, S.; Ishida, H.; Kosugi, Y.; Ishikawa, K.
    Hyperthermostable endoglucanase from Pyrococcus horikoshii (2002), Appl. Environ. Microbiol., 68, 430-433.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.4 industry because of its ability to hydrolyze celluloses at high temperatures above 90°C, as well as its thermostability, the enzyme is expected to be an excellent tool for industrial hydrolysis of cellulose, particularly for biopolishing of cotton products Pyrococcus horikoshii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expressed in Escherichia coli Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 43000
-
x * 43000, SDS-PAGE Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Pyrococcus horikoshii O58925
-
-
3.2.1.4 Pyrococcus horikoshii DSM 12428 O58925
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 avicel + H2O
-
Pyrococcus horikoshii ?
-
?
3.2.1.4 avicel + H2O
-
Pyrococcus horikoshii DSM 12428 ?
-
?
3.2.1.4 carboxymethyl cellulose + H2O
-
Pyrococcus horikoshii ?
-
?
3.2.1.4 carboxymethyl cellulose + H2O
-
Pyrococcus horikoshii DSM 12428 ?
-
?
3.2.1.4 lichenan + H2O
-
Pyrococcus horikoshii ?
-
?
3.2.1.4 lichenan + H2O
-
Pyrococcus horikoshii DSM 12428 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 43000, SDS-PAGE Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 PH1171 locus name Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 85
-
assay at Pyrococcus horikoshii
3.2.1.4 97
-
-
Pyrococcus horikoshii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 97
-
3 h, 20% loss of activity Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5.6
-
assay at Pyrococcus horikoshii