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Literature summary extracted from
- An improved procedure for the purification of catalytically active alkane hydroxylase from Pseudomonas putida GPo1 (2011), Appl. Biochem. Biotechnol., 165, 823-831.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.14.15.3 | environmental protection | the enzyme has a tremendous biotechnological potential as a biocatalyst and promising application in the bioremediation of oil-contaminated environments | Pseudomonas putida |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.15.3 | gene alkB, expression of C-terminally StrepII-tagged AlkB in membranes of Escherichia coli strain NovaBlue | Pseudomonas putida |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.15.3 | 0.2 | - |
1-octene | pH 7.4, 30°C, recombinant enzyme | Pseudomonas putida |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.15.3 | membrane | AlkB is an integral membrane monooxygenase | Pseudomonas putida | 16020 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.15.3 | Pseudomonas putida | Q9WWW6 | gene alkB | - |
| 1.14.15.3 | Pseudomonas putida GPo1 / ATCC29347 | Q9WWW6 | gene alkB | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.15.3 | recombinant C-terminally StrepII-tagged AlkB from membranes of Escherichia coli strain NovaBlue by detergent solubilization, and Strep-tag affinity chromatography | Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.15.3 | 1-octene + reduced rubredoxin + O2 | epoxidation reaction catalysed by AlkB | Pseudomonas putida | 1,2-epoxyoctane + oxidized rubredoxin + H2O | - |
? | |
| 1.14.15.3 | 1-octene + reduced rubredoxin + O2 | epoxidation reaction catalysed by AlkB | Pseudomonas putida GPo1 / ATCC29347 | 1,2-epoxyoctane + oxidized rubredoxin + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.15.3 | ? | x * 44000-46000, recombinant StrepII-tagged AlkB, SDS-PAGE | Pseudomonas putida |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.15.3 | AlkB | - |
Pseudomonas putida |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.3 | 30 | - |
assay at | Pseudomonas putida |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.3 | 7.4 | - |
assay at | Pseudomonas putida |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.3 | rubredoxin | endogenous, recombinantly expressed in Escherichia coli TG10 cells | Pseudomonas putida |
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Release 2023.1 (January 2023)
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