BRENDA - Enzyme Database

Isolation and characterization of a lipoxygenase from Pseudomonas 42A2 responsible for the biotransformation of oleic acid into (S)-(E)-10-hydroxy-8-octadecenoic acid

Busquets, M.; Deroncel, V.; Vidal-Mas, J.; Rodrguez, E.; Guerrero, A.; Manresa, A.; Antonie van Leeuwenhoek 85, 129-139 (2004)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.11.77
Ba2+
complete inhibition at 1 mM
Pseudomonas sp.
1.13.11.77
EDTA
99% inhibition at 5 mM
Pseudomonas sp.
1.13.11.77
Fe3+
complete inhibition at 1 mM
Pseudomonas sp.
1.13.11.77
Mg2+
concentrations of Mg2+ higher than 1.25 mM inhibit activity strongly (90% of activity lost at 1.75 mM)
Pseudomonas sp.
1.13.11.77
additional information
not inhibited by 5 mM KCN
Pseudomonas sp.
1.13.11.77
oleate
at concentrations of oleate higher than 2.1 mM, the enzyme activity is inhibited by excess of substrate
Pseudomonas sp.
1.13.11.77
Zn2+
complete inhibition at 1 mM
Pseudomonas sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.11.77
0.66
-
linoleate
in 0.2 M sodium borate buffer, pH 9.0, at 30C
Pseudomonas sp.
1.13.11.77
0.73
-
alpha-linolenate
in 0.2 M sodium borate buffer, pH 9.0, at 30C
Pseudomonas sp.
1.13.11.77
0.74
-
oleate
in 0.2 M sodium borate buffer, pH 9.0, at 30C
Pseudomonas sp.
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.13.11.77
periplasm
-
Pseudomonas sp.
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.13.11.77
Fe2+
the enzyme contains 0.55 mol of Fe2+ per mol of protein
Pseudomonas sp.
1.13.11.77
Mg2+
the presence of 1 mM Mg2+ increases enzyme activity 1.5fold
Pseudomonas sp.
1.13.11.77
Mn2+
the enzyme contains 25 mmol Mn2+ per mol of protein
Pseudomonas sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.77
45000
-
1 * 45000, SDS-PAGE
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.13.11.77
linoleate + O2
Pseudomonas sp.
100% activity
(8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
?
1.13.11.77
linoleate + O2
Pseudomonas sp. 42A2
100% activity
(8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
?
1.13.11.77
oleate + O2
Pseudomonas sp.
46% activity compared to linoleate
(8E,10S)-10-hydroperoxyoctadeca-8-enoate
-
-
?
1.13.11.77
oleate + O2
Pseudomonas sp. 42A2
46% activity compared to linoleate
(8E,10S)-10-hydroperoxyoctadeca-8-enoate
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.13.11.77
Pseudomonas sp.
-
-
-
1.13.11.77
Pseudomonas sp. 42A2
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.13.11.77
Q Sepharose column chromatography and Macro-Prep SE 100/40 gel filtration
Pseudomonas sp.
Storage Stability
EC Number
Storage Stability
Organism
1.13.11.77
4C, 1 week, no loss of activity (thawing and freezing decreases enzyme activity by 30%)
Pseudomonas sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.13.11.77
alpha-linolenate + O2
60.4% activity compared to linoleate
723962
Pseudomonas sp.
(8E,10S,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
-
-
-
?
1.13.11.77
alpha-linolenate + O2
60.4% activity compared to linoleate
723962
Pseudomonas sp. 42A2
(8E,10S,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
-
-
-
?
1.13.11.77
arachidonate + O2
0.9% activity compared to linoleate
723962
Pseudomonas sp.
?
-
-
-
?
1.13.11.77
arachidonate + O2
0.9% activity compared to linoleate
723962
Pseudomonas sp. 42A2
?
-
-
-
?
1.13.11.77
cis-12-octadecenoate + O2
8.9% activity compared to linoleate
723962
Pseudomonas sp.
?
-
-
-
?
1.13.11.77
linoleate + O2
100% activity
723962
Pseudomonas sp.
(8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
-
?
1.13.11.77
linoleate + O2
100% activity
723962
Pseudomonas sp. 42A2
(8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
-
?
1.13.11.77
additional information
the activity of the enzyme is highest in unsaturated fatty acids containing double bonds in position 9 (oleic, linoleic and linolenic acids), linoleic acid being preferred over linolenic and oleic acids
723962
Pseudomonas sp.
?
-
-
-
-
1.13.11.77
additional information
the activity of the enzyme is highest in unsaturated fatty acids containing double bonds in position 9 (oleic, linoleic and linolenic acids), linoleic acid being preferred over linolenic and oleic acids
723962
Pseudomonas sp. 42A2
?
-
-
-
-
1.13.11.77
oleate + O2
46% activity compared to linoleate
723962
Pseudomonas sp.
(8E,10S)-10-hydroperoxyoctadeca-8-enoate
-
-
-
?
1.13.11.77
oleate + O2
46% activity compared to linoleate
723962
Pseudomonas sp. 42A2
(8E,10S)-10-hydroperoxyoctadeca-8-enoate
-
-
-
?
1.13.11.77
petroselinoate + O2
26.4% activity compared to linoleate
723962
Pseudomonas sp.
?
-
-
-
?
1.13.11.77
triolein + O2
0.4% activity compared to linoleate
723962
Pseudomonas sp.
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.13.11.77
monomer
1 * 45000, SDS-PAGE
Pseudomonas sp.
Synonyms
EC Number
Synonyms
Commentary
Organism
1.13.11.77
LOX
-
Pseudomonas sp.
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.13.11.77
25
30
-
Pseudomonas sp.
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.13.11.77
45
70
44% of activity still remains at 55C. The enzyme is stable up to 45 C, but sharply decreases after 50C. Activity is completely lost at 70C
Pseudomonas sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.77
8.5
9
-
Pseudomonas sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.11.77
Ba2+
complete inhibition at 1 mM
Pseudomonas sp.
1.13.11.77
EDTA
99% inhibition at 5 mM
Pseudomonas sp.
1.13.11.77
Fe3+
complete inhibition at 1 mM
Pseudomonas sp.
1.13.11.77
Mg2+
concentrations of Mg2+ higher than 1.25 mM inhibit activity strongly (90% of activity lost at 1.75 mM)
Pseudomonas sp.
1.13.11.77
additional information
not inhibited by 5 mM KCN
Pseudomonas sp.
1.13.11.77
oleate
at concentrations of oleate higher than 2.1 mM, the enzyme activity is inhibited by excess of substrate
Pseudomonas sp.
1.13.11.77
Zn2+
complete inhibition at 1 mM
Pseudomonas sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.11.77
0.66
-
linoleate
in 0.2 M sodium borate buffer, pH 9.0, at 30C
Pseudomonas sp.
1.13.11.77
0.73
-
alpha-linolenate
in 0.2 M sodium borate buffer, pH 9.0, at 30C
Pseudomonas sp.
1.13.11.77
0.74
-
oleate
in 0.2 M sodium borate buffer, pH 9.0, at 30C
Pseudomonas sp.
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.13.11.77
periplasm
-
Pseudomonas sp.
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.13.11.77
Fe2+
the enzyme contains 0.55 mol of Fe2+ per mol of protein
Pseudomonas sp.
1.13.11.77
Mg2+
the presence of 1 mM Mg2+ increases enzyme activity 1.5fold
Pseudomonas sp.
1.13.11.77
Mn2+
the enzyme contains 25 mmol Mn2+ per mol of protein
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.77
45000
-
1 * 45000, SDS-PAGE
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.13.11.77
linoleate + O2
Pseudomonas sp.
100% activity
(8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
?
1.13.11.77
linoleate + O2
Pseudomonas sp. 42A2
100% activity
(8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
?
1.13.11.77
oleate + O2
Pseudomonas sp.
46% activity compared to linoleate
(8E,10S)-10-hydroperoxyoctadeca-8-enoate
-
-
?
1.13.11.77
oleate + O2
Pseudomonas sp. 42A2
46% activity compared to linoleate
(8E,10S)-10-hydroperoxyoctadeca-8-enoate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.77
Q Sepharose column chromatography and Macro-Prep SE 100/40 gel filtration
Pseudomonas sp.
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.13.11.77
4C, 1 week, no loss of activity (thawing and freezing decreases enzyme activity by 30%)
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.13.11.77
alpha-linolenate + O2
60.4% activity compared to linoleate
723962
Pseudomonas sp.
(8E,10S,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
-
-
-
?
1.13.11.77
alpha-linolenate + O2
60.4% activity compared to linoleate
723962
Pseudomonas sp. 42A2
(8E,10S,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
-
-
-
?
1.13.11.77
arachidonate + O2
0.9% activity compared to linoleate
723962
Pseudomonas sp.
?
-
-
-
?
1.13.11.77
arachidonate + O2
0.9% activity compared to linoleate
723962
Pseudomonas sp. 42A2
?
-
-
-
?
1.13.11.77
cis-12-octadecenoate + O2
8.9% activity compared to linoleate
723962
Pseudomonas sp.
?
-
-
-
?
1.13.11.77
linoleate + O2
100% activity
723962
Pseudomonas sp.
(8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
-
?
1.13.11.77
linoleate + O2
100% activity
723962
Pseudomonas sp. 42A2
(8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
-
?
1.13.11.77
additional information
the activity of the enzyme is highest in unsaturated fatty acids containing double bonds in position 9 (oleic, linoleic and linolenic acids), linoleic acid being preferred over linolenic and oleic acids
723962
Pseudomonas sp.
?
-
-
-
-
1.13.11.77
additional information
the activity of the enzyme is highest in unsaturated fatty acids containing double bonds in position 9 (oleic, linoleic and linolenic acids), linoleic acid being preferred over linolenic and oleic acids
723962
Pseudomonas sp. 42A2
?
-
-
-
-
1.13.11.77
oleate + O2
46% activity compared to linoleate
723962
Pseudomonas sp.
(8E,10S)-10-hydroperoxyoctadeca-8-enoate
-
-
-
?
1.13.11.77
oleate + O2
46% activity compared to linoleate
723962
Pseudomonas sp. 42A2
(8E,10S)-10-hydroperoxyoctadeca-8-enoate
-
-
-
?
1.13.11.77
petroselinoate + O2
26.4% activity compared to linoleate
723962
Pseudomonas sp.
?
-
-
-
?
1.13.11.77
triolein + O2
0.4% activity compared to linoleate
723962
Pseudomonas sp.
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.13.11.77
monomer
1 * 45000, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.13.11.77
25
30
-
Pseudomonas sp.
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.13.11.77
45
70
44% of activity still remains at 55C. The enzyme is stable up to 45 C, but sharply decreases after 50C. Activity is completely lost at 70C
Pseudomonas sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.77
8.5
9
-
Pseudomonas sp.