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High activity catechol 1,2-dioxygenase from Stenotrophomonas maltophilia strain KB2 as a useful tool in cis,cis-muconic acid production

Guzik, U.; Hupert-Kocurek, K.; Sitnik, M.; Wojcieszynska, D.; Antonie van Leeuwenhoek 103, 1297-1307 (2013)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.13.11.1
DNA and amino acid sequence determination and analysis, the gene encoding the enzyme is located on the chromosomal DNA, phylogenetic tree
Stenotrophomonas maltophilia
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.11.1
2,2'-dipyridyl
complete inhibition at 2 mM, 99.9% at 1 mM
Stenotrophomonas maltophilia
1.13.11.1
2,4-Dichlorophenol
26.0% inhibition at 0.1 mM, 29.8% at 0.3 mM
Stenotrophomonas maltophilia
1.13.11.1
2-chlorophenol
31.1% inhibition at 0.1 mM, 17% at 0.3 mM
Stenotrophomonas maltophilia
1.13.11.1
2-methylphenol
24.7% inhibition at 0.1 mM
Stenotrophomonas maltophilia
1.13.11.1
3-methylphenol
20.4% inhibition at 0.1 mM, 32% at 0.3 mM
Stenotrophomonas maltophilia
1.13.11.1
4-Chlorophenol
11% inhibition at 0.1 mM, 13% at 0.3 mM
Stenotrophomonas maltophilia
1.13.11.1
4-Methylphenol
8% inhibition at 0.1 mM, 27.5% at 0.3 mM
Stenotrophomonas maltophilia
1.13.11.1
catechol
substrate inhibition at over 0.08 mM substrate concentration
Stenotrophomonas maltophilia
1.13.11.1
EDTA
99.1 inhibition at 3 mM, 93.7% at 1 mM
Stenotrophomonas maltophilia
1.13.11.1
Phenanthroline
complete inhibition at 3 mM, 99% at 1 mM
Stenotrophomonas maltophilia
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.11.1
additional information
-
additional information
Michaelis-Menten kinetics, overview
Stenotrophomonas maltophilia
1.13.11.1
0.0128
-
catechol
pH 8.0, 40°C
Stenotrophomonas maltophilia
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.1
34500
-
x * 34500, aabout, sequence calculation
Stenotrophomonas maltophilia
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.13.11.1
catechol + O2
Stenotrophomonas maltophilia
-
cis,cis-muconate
-
-
?
1.13.11.1
catechol + O2
Stenotrophomonas maltophilia KB2
-
cis,cis-muconate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.13.11.1
Stenotrophomonas maltophilia
A7LBQ5
-
-
1.13.11.1
Stenotrophomonas maltophilia KB2
A7LBQ5
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.1
3-methylcatechol + O2
11.6% of the activity with catechol
723960
Stenotrophomonas maltophilia
3-methyl-cis,cis-muconate
-
-
-
?
1.13.11.1
3-methylcatechol + O2
11.6% of the activity with catechol
723960
Stenotrophomonas maltophilia KB2
3-methyl-cis,cis-muconate
-
-
-
?
1.13.11.1
4-methylcatechol + O2
23.0% of the activity with catechol
723960
Stenotrophomonas maltophilia
4-methyl-cis,cis-muconate
-
-
-
?
1.13.11.1
4-methylcatechol + O2
23.0% of the activity with catechol
723960
Stenotrophomonas maltophilia KB2
4-methyl-cis,cis-muconate
-
-
-
?
1.13.11.1
catechol + O2
-
723960
Stenotrophomonas maltophilia
cis,cis-muconate
-
-
-
?
1.13.11.1
catechol + O2
best substrate
723960
Stenotrophomonas maltophilia
cis,cis-muconate
-
-
-
?
1.13.11.1
catechol + O2
-
723960
Stenotrophomonas maltophilia KB2
cis,cis-muconate
-
-
-
?
1.13.11.1
catechol + O2
best substrate
723960
Stenotrophomonas maltophilia KB2
cis,cis-muconate
-
-
-
?
1.13.11.1
additional information
substrate specificity, overview. No activity with 3-chlorocatechol, 4-chlorocatechol, 3,5-dichlorocatechol, 4,5-dichlorocatechol, and hydroquinone
723960
Stenotrophomonas maltophilia
?
-
-
-
-
1.13.11.1
additional information
substrate specificity, overview. No activity with 3-chlorocatechol, 4-chlorocatechol, 3,5-dichlorocatechol, 4,5-dichlorocatechol, and hydroquinone
723960
Stenotrophomonas maltophilia KB2
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.13.11.1
?
x * 34500, aabout, sequence calculation
Stenotrophomonas maltophilia
1.13.11.1
More
three-dimensional structure deduction, molecular modeling, overview
Stenotrophomonas maltophilia
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.13.11.1
40
-
-
Stenotrophomonas maltophilia
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.13.11.1
4
50
activity range, profile overview
Stenotrophomonas maltophilia
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.13.11.1
40
-
half-life is 3 h
Stenotrophomonas maltophilia
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.1
8
-
-
Stenotrophomonas maltophilia
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.13.11.1
3
12
activity range, profile overview
Stenotrophomonas maltophilia
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.1
DNA and amino acid sequence determination and analysis, the gene encoding the enzyme is located on the chromosomal DNA, phylogenetic tree
Stenotrophomonas maltophilia
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.11.1
2,2'-dipyridyl
complete inhibition at 2 mM, 99.9% at 1 mM
Stenotrophomonas maltophilia
1.13.11.1
2,4-Dichlorophenol
26.0% inhibition at 0.1 mM, 29.8% at 0.3 mM
Stenotrophomonas maltophilia
1.13.11.1
2-chlorophenol
31.1% inhibition at 0.1 mM, 17% at 0.3 mM
Stenotrophomonas maltophilia
1.13.11.1
2-methylphenol
24.7% inhibition at 0.1 mM
Stenotrophomonas maltophilia
1.13.11.1
3-methylphenol
20.4% inhibition at 0.1 mM, 32% at 0.3 mM
Stenotrophomonas maltophilia
1.13.11.1
4-Chlorophenol
11% inhibition at 0.1 mM, 13% at 0.3 mM
Stenotrophomonas maltophilia
1.13.11.1
4-Methylphenol
8% inhibition at 0.1 mM, 27.5% at 0.3 mM
Stenotrophomonas maltophilia
1.13.11.1
catechol
substrate inhibition at over 0.08 mM substrate concentration
Stenotrophomonas maltophilia
1.13.11.1
EDTA
99.1 inhibition at 3 mM, 93.7% at 1 mM
Stenotrophomonas maltophilia
1.13.11.1
Phenanthroline
complete inhibition at 3 mM, 99% at 1 mM
Stenotrophomonas maltophilia
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.11.1
additional information
-
additional information
Michaelis-Menten kinetics, overview
Stenotrophomonas maltophilia
1.13.11.1
0.0128
-
catechol
pH 8.0, 40°C
Stenotrophomonas maltophilia
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.1
34500
-
x * 34500, aabout, sequence calculation
Stenotrophomonas maltophilia
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.13.11.1
catechol + O2
Stenotrophomonas maltophilia
-
cis,cis-muconate
-
-
?
1.13.11.1
catechol + O2
Stenotrophomonas maltophilia KB2
-
cis,cis-muconate
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.1
3-methylcatechol + O2
11.6% of the activity with catechol
723960
Stenotrophomonas maltophilia
3-methyl-cis,cis-muconate
-
-
-
?
1.13.11.1
3-methylcatechol + O2
11.6% of the activity with catechol
723960
Stenotrophomonas maltophilia KB2
3-methyl-cis,cis-muconate
-
-
-
?
1.13.11.1
4-methylcatechol + O2
23.0% of the activity with catechol
723960
Stenotrophomonas maltophilia
4-methyl-cis,cis-muconate
-
-
-
?
1.13.11.1
4-methylcatechol + O2
23.0% of the activity with catechol
723960
Stenotrophomonas maltophilia KB2
4-methyl-cis,cis-muconate
-
-
-
?
1.13.11.1
catechol + O2
-
723960
Stenotrophomonas maltophilia
cis,cis-muconate
-
-
-
?
1.13.11.1
catechol + O2
best substrate
723960
Stenotrophomonas maltophilia
cis,cis-muconate
-
-
-
?
1.13.11.1
catechol + O2
-
723960
Stenotrophomonas maltophilia KB2
cis,cis-muconate
-
-
-
?
1.13.11.1
catechol + O2
best substrate
723960
Stenotrophomonas maltophilia KB2
cis,cis-muconate
-
-
-
?
1.13.11.1
additional information
substrate specificity, overview. No activity with 3-chlorocatechol, 4-chlorocatechol, 3,5-dichlorocatechol, 4,5-dichlorocatechol, and hydroquinone
723960
Stenotrophomonas maltophilia
?
-
-
-
-
1.13.11.1
additional information
substrate specificity, overview. No activity with 3-chlorocatechol, 4-chlorocatechol, 3,5-dichlorocatechol, 4,5-dichlorocatechol, and hydroquinone
723960
Stenotrophomonas maltophilia KB2
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.13.11.1
?
x * 34500, aabout, sequence calculation
Stenotrophomonas maltophilia
1.13.11.1
More
three-dimensional structure deduction, molecular modeling, overview
Stenotrophomonas maltophilia
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.13.11.1
40
-
-
Stenotrophomonas maltophilia
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.13.11.1
4
50
activity range, profile overview
Stenotrophomonas maltophilia
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.13.11.1
40
-
half-life is 3 h
Stenotrophomonas maltophilia
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.1
8
-
-
Stenotrophomonas maltophilia
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.13.11.1
3
12
activity range, profile overview
Stenotrophomonas maltophilia
General Information
EC Number
General Information
Commentary
Organism
1.13.11.1
additional information
three-dimensional structure deduction, molecular modeling, overview
Stenotrophomonas maltophilia
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.13.11.1
additional information
three-dimensional structure deduction, molecular modeling, overview
Stenotrophomonas maltophilia