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Literature summary extracted from

  • Guzik, U.; Hupert-Kocurek, K.; Sitnik, M.; Wojcieszynska, D.
    High activity catechol 1,2-dioxygenase from Stenotrophomonas maltophilia strain KB2 as a useful tool in cis,cis-muconic acid production (2013), Antonie van Leeuwenhoek, 103, 1297-1307.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.1 DNA and amino acid sequence determination and analysis, the gene encoding the enzyme is located on the chromosomal DNA, phylogenetic tree Stenotrophomonas maltophilia

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.1 2,2'-dipyridyl complete inhibition at 2 mM, 99.9% at 1 mM Stenotrophomonas maltophilia
1.13.11.1 2,4-Dichlorophenol 26.0% inhibition at 0.1 mM, 29.8% at 0.3 mM Stenotrophomonas maltophilia
1.13.11.1 2-chlorophenol 31.1% inhibition at 0.1 mM, 17% at 0.3 mM Stenotrophomonas maltophilia
1.13.11.1 2-methylphenol 24.7% inhibition at 0.1 mM Stenotrophomonas maltophilia
1.13.11.1 3-methylphenol 20.4% inhibition at 0.1 mM, 32% at 0.3 mM Stenotrophomonas maltophilia
1.13.11.1 4-Chlorophenol 11% inhibition at 0.1 mM, 13% at 0.3 mM Stenotrophomonas maltophilia
1.13.11.1 4-Methylphenol 8% inhibition at 0.1 mM, 27.5% at 0.3 mM Stenotrophomonas maltophilia
1.13.11.1 catechol substrate inhibition at over 0.08 mM substrate concentration Stenotrophomonas maltophilia
1.13.11.1 EDTA 99.1 inhibition at 3 mM, 93.7% at 1 mM Stenotrophomonas maltophilia
1.13.11.1 Phenanthroline complete inhibition at 3 mM, 99% at 1 mM Stenotrophomonas maltophilia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.1 additional information
-
 additional information Michaelis-Menten kinetics, overview Stenotrophomonas maltophilia
1.13.11.1 0.0128
-
 catechol pH 8.0, 40°C Stenotrophomonas maltophilia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.1 34500
-
 x * 34500, aabout, sequence calculation Stenotrophomonas maltophilia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.1 catechol + O2 Stenotrophomonas maltophilia
-
 cis,cis-muconate
-
 ?
1.13.11.1 catechol + O2 Stenotrophomonas maltophilia KB2
-
 cis,cis-muconate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Stenotrophomonas maltophilia A7LBQ5
-
-
1.13.11.1 Stenotrophomonas maltophilia KB2 A7LBQ5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 3-methylcatechol + O2 11.6% of the activity with catechol Stenotrophomonas maltophilia 2-methyl-cis,cis-muconate
-
 ?
1.13.11.1 3-methylcatechol + O2 11.6% of the activity with catechol Stenotrophomonas maltophilia KB2 2-methyl-cis,cis-muconate
-
 ?
1.13.11.1 4-methylcatechol + O2 23.0% of the activity with catechol Stenotrophomonas maltophilia 4-methyl-cis,cis-muconate
-
 ?
1.13.11.1 4-methylcatechol + O2 23.0% of the activity with catechol Stenotrophomonas maltophilia KB2 4-methyl-cis,cis-muconate
-
 ?
1.13.11.1 catechol + O2
-
 Stenotrophomonas maltophilia cis,cis-muconate
-
 ?
1.13.11.1 catechol + O2 best substrate Stenotrophomonas maltophilia cis,cis-muconate
-
 ?
1.13.11.1 catechol + O2
-
 Stenotrophomonas maltophilia KB2 cis,cis-muconate
-
 ?
1.13.11.1 catechol + O2 best substrate Stenotrophomonas maltophilia KB2 cis,cis-muconate
-
 ?
1.13.11.1 additional information substrate specificity, overview. No activity with 3-chlorocatechol, 4-chlorocatechol, 3,5-dichlorocatechol, 4,5-dichlorocatechol, and hydroquinone Stenotrophomonas maltophilia ?
-
 ?
1.13.11.1 additional information substrate specificity, overview. No activity with 3-chlorocatechol, 4-chlorocatechol, 3,5-dichlorocatechol, 4,5-dichlorocatechol, and hydroquinone Stenotrophomonas maltophilia KB2 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.1 ? x * 34500, aabout, sequence calculation Stenotrophomonas maltophilia
1.13.11.1 More three-dimensional structure deduction, molecular modeling, overview Stenotrophomonas maltophilia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.1 40
-
-
 Stenotrophomonas maltophilia

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.1 4 50 activity range, profile overview Stenotrophomonas maltophilia

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.1 40
-
 half-life is 3 h Stenotrophomonas maltophilia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.1 8
-
-
 Stenotrophomonas maltophilia

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.1 3 12 activity range, profile overview Stenotrophomonas maltophilia

General Information

EC Number General Information Comment Organism
1.13.11.1 additional information three-dimensional structure deduction, molecular modeling, overview Stenotrophomonas maltophilia