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Literature summary extracted from

  • Jiang, C.; Yin, B.; Tang, M.; Zhao, G.; He, J.; Shen, P.; Wu, B.
    Identification of a metagenome-derived prephenate dehydrogenase gene from an alkaline-polluted soil microorganism (2013), Antonie van Leeuwenhoek, 103, 1209-1219.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.12 expression in Escherichia coli uncultured bacterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.12 additional information not inhibitory: tyrosine uncultured bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.12 0.87
-
 prephenate pH 8.0, 45°C uncultured bacterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.12 Cu2+ 0.5 mM, 56.8% residual activity uncultured bacterium
1.3.1.12 Fe3+ 0.5 mM, 58.7% residual activity uncultured bacterium
1.3.1.12 Mn2+ 0.5 mM, 72% residual activity uncultured bacterium
1.3.1.12 SDS 0.10%, 18.3% residual activity uncultured bacterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.12 31400
-
 x * 31400, calculated uncultured bacterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.12 prephenate + NAD+ uncultured bacterium
-
 4-hydroxyphenylpyruvate + NADH + H+ + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.12 uncultured bacterium J9XQS6 uncultured alkaline-polluted bacterium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.12 recombinant enzyme uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.12 prephenate + NAD+
-
 uncultured bacterium 4-hydroxyphenylpyruvate + NADH + H+ + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.12 ? x * 31400, calculated uncultured bacterium

Synonyms

EC Number Synonyms Comment Organism
1.3.1.12 pdhE-1
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.12 45
-
-
 uncultured bacterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.3.1.12 35 50 more than 80% of maximum activity within uncultured bacterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.12 10.08
-
 prephenate pH 8.0, 45°C uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.12 8
-
-
 uncultured bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.12 6 8.5 more than 75% of maximum activity within uncultured bacterium

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.3.1.12 uncultured bacterium
-
-
 5.5

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3.1.12 11.6
-
 prephenate pH 8.0, 45°C uncultured bacterium