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Literature summary extracted from
- A simple assay of taurine concentrations in food and biological samples using taurine dioxygenase (2012), Anal. Biochem., 427, 121-123.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.14.11.17 | analysis | development of a colorimetric assay method for determination of taurine in commercially available beverages and some biological samples using the taurine dioxygenase. Taurine determination in food control, biological research, and diagnoses based on urinary taurine concentration | Escherichia coli |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.11.17 | expression of His-tagged TauD in Escherichia coli strain BL21(DE3) | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.11.17 | EDTA | - |
Escherichia coli |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.11.17 | Fe2+ | required | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.11.17 | taurine + 2-oxoglutarate + O2 | Escherichia coli | - |
sulfite + aminoacetaldehyde + succinate + CO2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.11.17 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.11.17 | recombinant His-tagged TauD from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.11.17 | taurine + 2-oxoglutarate + O2 | - |
Escherichia coli | sulfite + aminoacetaldehyde + succinate + CO2 | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.11.17 | 30 | - |
assay at | Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.11.17 | 7 | - |
assay at | Escherichia coli |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.11.17 | additional information | development of a colorimetric assay method, based on the measurement of sulfite using Ellman's reagent, i.e. 2.5 mM 5,5'-dithiobis(2-nitrobenzoic acid), for determination of taurine in commercially available beverages and some biological samples, overview | Escherichia coli |
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Release 2023.1 (January 2023)
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