EC Number | Cloned (Comment) | Organism |
---|---|---|
2.8.2.20 | substance protein A-fused tyrosylprotein sulfotransferase is expressed in Escherichia coli BL21(DE3) cells | Drosophila melanogaster |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.8.2.20 | 3'-phosphoadenylyl sulfate | - |
Drosophila melanogaster |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.8.2.20 | 0.0021 | - |
3'-phosphoadenylyl sulfate | using glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C | Drosophila melanogaster | |
2.8.2.20 | 0.011 | - |
P-selectin glycoprotein ligand-1 | using glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C | Drosophila melanogaster | |
2.8.2.20 | 0.016 | - |
3'-phosphoadenylyl sulfate | using P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C | Drosophila melanogaster | |
2.8.2.20 | 0.053 | - |
P-selectin glycoprotein ligand-1 | using P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C | Drosophila melanogaster |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.2.20 | 3'-phosphoadenylyl sulfate + glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 | Drosophila melanogaster | - |
adenosine 3',5'-bisphosphate + glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 tyrosine O-sulfate | - |
? | |
2.8.2.20 | 3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1 | Drosophila melanogaster | i.e. ATEYEYLDYDFL | adenosine 3',5'-bisphosphate + P-selectin glycoprotein ligand-1 tyrosine O-sulfate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.8.2.20 | Drosophila melanogaster | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.2.20 | 3'-phosphoadenylyl sulfate + glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 | - |
Drosophila melanogaster | adenosine 3',5'-bisphosphate + glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 tyrosine O-sulfate | - |
? | |
2.8.2.20 | 3'-phosphoadenylyl sulfate + P-selectin glycoprotein ligand-1 | i.e. ATEYEYLDYDFL | Drosophila melanogaster | adenosine 3',5'-bisphosphate + P-selectin glycoprotein ligand-1 tyrosine O-sulfate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.8.2.20 | TPST | - |
Drosophila melanogaster |
2.8.2.20 | tyrosylprotein sulfotransferase | - |
Drosophila melanogaster |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.8.2.20 | 0.045 | - |
P-selectin glycoprotein ligand-1 | using glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C | Drosophila melanogaster | |
2.8.2.20 | 0.058 | - |
3'-phosphoadenylyl sulfate | using glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C | Drosophila melanogaster | |
2.8.2.20 | 0.1 | - |
P-selectin glycoprotein ligand-1 | using P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C | Drosophila melanogaster | |
2.8.2.20 | 0.11 | - |
3'-phosphoadenylyl sulfate | using P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C | Drosophila melanogaster |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.8.2.20 | 0.031 | - |
3'-phosphoadenylyl sulfate | using glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 as substrate,in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C | Drosophila melanogaster | |
2.8.2.20 | 0.061 | - |
3'-phosphoadenylyl sulfate | using P-selectin glycoprotein ligand-1 as substrate,in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C | Drosophila melanogaster |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.8.2.20 | 2 | - |
P-selectin glycoprotein ligand-1 | using P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C | Drosophila melanogaster | |
2.8.2.20 | 4.2 | - |
P-selectin glycoprotein ligand-1 | using glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C | Drosophila melanogaster | |
2.8.2.20 | 6.7 | - |
3'-phosphoadenylyl sulfate | using P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C | Drosophila melanogaster | |
2.8.2.20 | 18.2 | - |
3'-phosphoadenylyl sulfate | using glutathione S-transferase-tagged P-selectin glycoprotein ligand-1 as cosubstrate, in 50 mM MES buffer (pH 6.5), 5 mM 2-mercaptoethanol, at 37°C | Drosophila melanogaster |