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Literature summary extracted from

  • Petrollino, D.; Forlani, G.
    Coenzyme preference of Streptococcus pyogenes delta1-pyrroline-5-carboxylate reductase: evidence supporting NADPH as the physiological electron donor (2012), Amino Acids, 43, 493-497.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.2 expressed in Escherichia coli BL21(DE3) pLysS cells Streptococcus pyogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.2 EDTA
-
 Streptococcus pyogenes
1.5.1.2 additional information not inhibited by NADPH Streptococcus pyogenes
1.5.1.2 NADH slight inhibition with 25% reduction of the catalytic rate at 200 mM Streptococcus pyogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.2 0.012
-
 NADPH apparent value, at pH 7.5 and 37°C Streptococcus pyogenes
1.5.1.2 0.176
-
 NADH apparent value, at pH 7.5 and 37°C Streptococcus pyogenes
1.5.1.2 0.184
-
 1-pyrroline-5-carboxylate apparent value, with NADPH as cosubstrate, at pH 7.5 and 37°C Streptococcus pyogenes
1.5.1.2 0.349
-
 1-pyrroline-5-carboxylate apparent value, with NADH as cosubstrate, at pH 7.5 and 37°C Streptococcus pyogenes

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.2 Streptococcus pyogenes
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.2 1-pyrroline-5-carboxylate + NADH + H+
-
 Streptococcus pyogenes L-proline + NAD+
-
 ?
1.5.1.2 1-pyrroline-5-carboxylate + NADPH + H+
-
 Streptococcus pyogenes L-proline + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.2 DELTA1-pyrroline-5-carboxylate reductase
-
 Streptococcus pyogenes
1.5.1.2 P5CR
-
 Streptococcus pyogenes

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.2 340
-
 NADPH at pH 7.5 and 37°C Streptococcus pyogenes
1.5.1.2 620
-
 NADH at pH 7.5 and 37°C Streptococcus pyogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.2 7.2 7.8
-
 Streptococcus pyogenes

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.2 NADH with NADH the activity is markedly inhibited by physiological levels of NADP+, so NADH cannot serve as electron donor in vivo Streptococcus pyogenes
1.5.1.2 NADPH
-
 Streptococcus pyogenes

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.5.1.2 29
-
 at pH 7.5 and 37°C Streptococcus pyogenes EDTA