Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Forlani, G.; Petrollino, D.; Fusetti, M.; Romanini, L.; Nocek, B.; Joachimiak, A.; Berlicki, L.; Kafarski, P.
    DELTA1-pyrroline-5-carboxylate reductase as a new target for therapeutics: inhibition of the enzyme from Streptococcus pyogenes and effects in vivo (2012), Amino Acids, 42, 2283-2291.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.2 ([[3,5-bis(trifluoromethyl)phenyl]amino]methanediyl)bis(phosphonic acid) 71.2% inhibition at 0.2 mM Streptococcus pyogenes
1.5.1.2 [[(2,3-dichlorophenyl)amino]methanediyl]bis(phosphonic acid) 95.4% inhibition at 0.2 mM Streptococcus pyogenes
1.5.1.2 [[(2,4-dichlorophenyl)amino]methanediyl]bis(phosphonic acid) 52% inhibition at 0.2 mM Streptococcus pyogenes
1.5.1.2 [[(2,5-dichlorophenyl)amino]methanediyl]bis(phosphonic acid) 63.9% inhibition at 0.2 mM Streptococcus pyogenes
1.5.1.2 [[(2,6-dichlorophenyl)amino]methanediyl]bis(phosphonic acid) 96.2% inhibition at 0.2 mM Streptococcus pyogenes
1.5.1.2 [[(3,4-dichlorophenyl)amino]methanediyl]bis(phosphonic acid) 92.5% inhibition at 0.2 mM Streptococcus pyogenes
1.5.1.2 [[(3,5-dibromophenyl)amino]methanediyl]bis(phosphonic acid) 98.4% inhibition at 0.2 mM Streptococcus pyogenes
1.5.1.2 [[(3,5-dichlorophenyl)amino]methanediyl]bis(phosphonic acid) 91.1% inhibition at 0.2 mM Streptococcus pyogenes
1.5.1.2 [[(3,5-dimethylphenyl)amino]methanediyl]bis(phosphonic acid) 70.5% inhibition at 0.2 mM Streptococcus pyogenes
1.5.1.2 [[(3-chlorophenyl)amino]methanediyl]bis(phosphonic acid) 71.2% inhibition at 0.2 mM Streptococcus pyogenes
1.5.1.2 [[(4-benzylphenyl)amino]methanediyl]bis(phosphonic acid) 94.5% inhibition at 0.2 mM Streptococcus pyogenes
1.5.1.2 [[(4-chlorophenyl)amino]methanediyl]bis(phosphonic acid) 61.5% inhibition at 0.2 mM Streptococcus pyogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.2 1-pyrroline-5-carboxylate + NAD(P)H + H+ Streptococcus pyogenes
-
 L-proline + NAD(P)+
-
 ?
1.5.1.2 1-pyrroline-5-carboxylate + NAD(P)H + H+ Streptococcus pyogenes ATCC 19615
-
 L-proline + NAD(P)+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.2 Streptococcus pyogenes
-
-
-
1.5.1.2 Streptococcus pyogenes ATCC 19615
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.2 nickel affinity gel column chromatography Streptococcus pyogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.2 1-pyrroline-5-carboxylate + NAD(P)H + H+
-
 Streptococcus pyogenes L-proline + NAD(P)+
-
 ?
1.5.1.2 1-pyrroline-5-carboxylate + NAD(P)H + H+
-
 Streptococcus pyogenes ATCC 19615 L-proline + NAD(P)+
-
 ?
1.5.1.2 1-pyrroline-5-carboxylate + NADH + H+
-
 Streptococcus pyogenes L-proline + NAD+
-
 ?
1.5.1.2 1-pyrroline-5-carboxylate + NADH + H+
-
 Streptococcus pyogenes ATCC 19615 L-proline + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.2 DELTA1-pyrroline-5-carboxylate reductase
-
 Streptococcus pyogenes
1.5.1.2 P5C reductase
-
 Streptococcus pyogenes

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.2 NADH
-
 Streptococcus pyogenes
1.5.1.2 NADPH
-
 Streptococcus pyogenes

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.5.1.2 0.00022
-
 pH 7.5-8.0, at 37°C Streptococcus pyogenes [[(3,5-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
1.5.1.2 0.00022
-
 pH 7.5-8.0, at 37°C Streptococcus pyogenes [[(3,5-dibromophenyl)amino]methanediyl]bis(phosphonic acid)
1.5.1.2 0.00039
-
 pH 7.5-8.0, at 37°C Streptococcus pyogenes [[(3,4-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
1.5.1.2 0.00041
-
 pH 7.5-8.0, at 37°C Streptococcus pyogenes [[(4-benzylphenyl)amino]methanediyl]bis(phosphonic acid)
1.5.1.2 0.00078
-
 pH 7.5-8.0, at 37°C Streptococcus pyogenes ([[3,5-bis(trifluoromethyl)phenyl]amino]methanediyl)bis(phosphonic acid)
1.5.1.2 0.00087
-
 pH 7.5-8.0, at 37°C Streptococcus pyogenes [[(2,3-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
1.5.1.2 0.00088
-
 pH 7.5-8.0, at 37°C Streptococcus pyogenes [[(2,6-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
1.5.1.2 0.0009
-
 pH 7.5-8.0, at 37°C Streptococcus pyogenes [[(3-chlorophenyl)amino]methanediyl]bis(phosphonic acid)
1.5.1.2 0.0014
-
 pH 7.5-8.0, at 37°C Streptococcus pyogenes [[(4-chlorophenyl)amino]methanediyl]bis(phosphonic acid)
1.5.1.2 0.0027
-
 pH 7.5-8.0, at 37°C Streptococcus pyogenes [[(2,5-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
1.5.1.2 0.012
-
 pH 7.5-8.0, at 37°C Streptococcus pyogenes [[(2,4-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
1.5.1.2 0.0256
-
 pH 7.5-8.0, at 37°C Streptococcus pyogenes [[(3,5-dimethylphenyl)amino]methanediyl]bis(phosphonic acid)