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Literature summary extracted from

  • Kaswurm, V.; Nguyen, T.T.; Maischberger, T.; Kulbe, K.D.; Michlmayr, H.
    Evaluation of the food grade expression systems NICE and pSIP for the production of 2,5-diketo-D-gluconic acid reductase from Corynebacterium glutamicum (2013), AMB Express, 3, 7-7.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.274 gene dkr, recombinant expression in Lactococcus lactis subsp. cremoris strains MG1363 or NZ3900 and Lactobacillus plantarum strain WCFS1 using food-grade vectors Corynebacterium glutamicum

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.274 additional information evaluation of the food grade expression systems NICE, Lactococcus lactis, and pSIP, Lactobacillus plantarum, for the production of 2,5-diketo-D-gluconic acid reductase from Corynebacterium glutamicum that also satisfies food safety requirements. Both systems are suitable for 2,5-DKG reductase expression, maximum production yields are obtained with Lactobacillus plantarum/pSIP609 by pH control at 6.5, overview Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.274 2,5-didehydro-D-gluconate + NADPH + H+ Corynebacterium glutamicum
-
 2-dehydro-D-gluconate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.274 Corynebacterium glutamicum H9CWC0 gene dkr
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.274 2,5-didehydro-D-gluconate + NADPH + H+
-
 Corynebacterium glutamicum 2-dehydro-D-gluconate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.274 2,5-diketo-D-gluconic acid reductase
-
 Corynebacterium glutamicum
1.1.1.274 2,5-DKG reductase
-
 Corynebacterium glutamicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.274 NADPH
-
 Corynebacterium glutamicum

General Information

EC Number General Information Comment Organism
1.1.1.274 physiological function 2,5-diketo-D-gluconic acid reductase catalyses the reduction of 2,5-diketo-D-gluconic acid to 2-keto-L-gulonic acid, a direct precursor (lactone) of L-ascorbic acid (vitamin C). This reaction is an essential step in the biocatalytic production of the food supplement vitamin C from D-glucose or D-gluconic acid Corynebacterium glutamicum