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Literature summary extracted from

  • Groebe, G.; Ullrich, R.; Pecyna, M.J.; Kapturska, D.; Friedrich, S.; Hofrichter, M.; Scheibner, K.
    High-yield production of aromatic peroxygenase by the agaric fungus Marasmius rotula (2011), AMB Express, 1, 31.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.2.1 0.071
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 25°C Marasmius rotula
1.11.2.1 0.118
-
 benzyl alcohol pH 5.5, 25°C Marasmius rotula
1.11.2.1 0.133
-
 2,6-dimethoxyphenol pH 5.5, 25°C Marasmius rotula
1.11.2.1 0.279
-
 veratryl alcohol pH 5.5, 25°C Marasmius rotula
1.11.2.1 0.791
-
 Naphthalene pH 5.5, 25°C Marasmius rotula
1.11.2.1 3.14
-
 H2O2 pH 5.5, 25°C Marasmius rotula

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.2.1 32000
-
 x * 32000, SDS-PAGE Marasmius rotula

Organism

EC Number Organism UniProt Comment Textmining
1.11.2.1 Marasmius rotula
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.2.1 glycoprotein molecular mass after deglycosylation: 27000 Da Marasmius rotula

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.2.1
-
 Marasmius rotula

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.2.1 culture medium high expression in carbon- and nitrogen-rich media Marasmius rotula
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.2.1 2 2,6-dimethoxyphenol + 2 H2O2
-
 Marasmius rotula coerulignone + 2 H2O
-
 ?
1.11.2.1 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Marasmius rotula ? + H2O
-
 ?
1.11.2.1 benzyl alcohol + H2O2
-
 Marasmius rotula benzaldehyde + H2O
-
 ?
1.11.2.1 additional information enzyme shows neither brominating nor chlorinating activities Marasmius rotula ?
-
 ?
1.11.2.1 naphthalene + H2O2
-
 Marasmius rotula ? + H2O
-
 ?
1.11.2.1 veratryl alcohol + H2O2 + H+
-
 Marasmius rotula veratraldehyde + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.2.1 ? x * 32000, SDS-PAGE Marasmius rotula

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.2.1 25
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 25°C Marasmius rotula
1.11.2.1 33
-
 Naphthalene pH 5.5, 25°C Marasmius rotula
1.11.2.1 49
-
 veratryl alcohol pH 5.5, 25°C Marasmius rotula
1.11.2.1 62
-
 benzyl alcohol pH 5.5, 25°C Marasmius rotula
1.11.2.1 70
-
 2,6-dimethoxyphenol pH 5.5, 25°C Marasmius rotula
1.11.2.1 76
-
 H2O2 pH 5.5, 25°C Marasmius rotula

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.2.1 5 6
-
 Marasmius rotula

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.2.1 5 10 highly stable within Marasmius rotula

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.2.1 Marasmius rotula three pI-bands between 5.0 and 5.3, major band at 5.3 5.3 5

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.2.1 24.2
-
 H2O2 pH 5.5, 25°C Marasmius rotula
1.11.2.1 42.5
-
 Naphthalene pH 5.5, 25°C Marasmius rotula
1.11.2.1 176
-
 veratryl alcohol pH 5.5, 25°C Marasmius rotula
1.11.2.1 353
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 25°C Marasmius rotula
1.11.2.1 525
-
 benzyl alcohol pH 5.5, 25°C Marasmius rotula
1.11.2.1 529
-
 2,6-dimethoxyphenol pH 5.5, 25°C Marasmius rotula