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Literature summary extracted from

  • Wollam, J.; Magomedova, L.; Magner, D.B.; Shen, Y.; Rottiers, V.; Motola, D.L.; Mangelsdorf, D.J.; Cummins, C.L.; Antebi, A.
    The Rieske oxygenase DAF-36 functions as a cholesterol 7-desaturase in steroidogenic pathways governing longevity (2011), Aging Cell, 10, 879-884.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.21 expression in Sf9 cell Caenorhabditis elegans
1.14.19.21 expressed in Sf9 cells Caenorhabditis elegans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.21 microsome
-
 Caenorhabditis elegans
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.21 cholesterol + O2 + NAD(P)H + H+ Caenorhabditis elegans first committed step in biosynthesis of Caenorhabditis elegans bile acid-like steroids called dafachronic acids cholesta-5,7-dien-3beta-ol + NAD(P)+ + 2 H2O
-
 ?
1.14.19.21 cholesterol + O2 + NADPH + H+ Caenorhabditis elegans
-
 cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.21 Caenorhabditis elegans Q17938
-
-
1.14.19.21 Caenorhabditis elegans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.21 cholesterol + O2 + NAD(P)H + H+ first committed step in biosynthesis of Caenorhabditis elegans bile acid-like steroids called dafachronic acids Caenorhabditis elegans cholesta-5,7-dien-3beta-ol + NAD(P)+ + 2 H2O
-
 ?
1.14.19.21 cholesterol + O2 + NADPH + H+
-
 Caenorhabditis elegans cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.21 DAF-36
-
 Caenorhabditis elegans
1.14.19.21 DAF-36
-
 Caenorhabditis elegans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.21 NADPH
-
 Caenorhabditis elegans

General Information

EC Number General Information Comment Organism
1.3.1.21 physiological function by controlling dafachronic acid production, enzyme DAF-36 regulates activities of nuclear receptor DAF-12 for reproductive development and longevity. Mutants deficient for the enzyme lack 7-dehydrocholesterol, revealing a 6.5fold decrease relative to wild-type, and accumulate the putative precursor, cholesterol, by 3fold. The ligand of nuclear receptor DAF-12, DELTA7-dafachronic acid, is also undetectable in the mutants, while DELTA4-dafachronic acid is below the detection limit in both wild-type and mutant Caenorhabditis elegans
1.14.19.21 physiological function the enzyme regulates DAF-12 activities for reproductive development and longevity by controlling dafachronic acids production Caenorhabditis elegans