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Literature summary extracted from

  • Van Der Horst, M.; Van Lieshout, J.; Bury, A.; Hartog, A.; Wever, R.
    Sulfation of various alcoholic groups by an arylsulfate sulfotransferase from Desulfitobacterium hafniense and synthesis of estradiol sulfate (2012), Adv. Synth. Catal., 354, 3501-3508.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.22 expressed in Escherichia coli BL21(DE3)cells Desulfitobacterium hafniense

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.22 acetone in acetone the activity of the enzyme decreases to 30-34% of the original activity Desulfitobacterium hafniense
2.8.2.22 dimethylformamide in dimethylformamide the activity of the enzyme decreases to 30-34% of the original activity Desulfitobacterium hafniense
2.8.2.22 dioxane in dioxane the activity of the enzyme decreases to 30-34% of the original activity Desulfitobacterium hafniense
2.8.2.22 tetrahydrofuran in tetrhydrofuran the activity of the enzyme decreases to 30-34% of the original activity Desulfitobacterium hafniense

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.22 70000
-
 x * 70000, SDS-PAGE Desulfitobacterium hafniense
2.8.2.22 71400
-
 x * 71400, calculated from amino acid sequence Desulfitobacterium hafniense

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.22 4-nitrophenyl sulfate + phenol Desulfitobacterium hafniense 100% activity 4-nitrophenol + phenyl sulfate
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.8.2.22 DMSO the enzyme is stable in the presence of 10% (v/v) of DMSO and even in 25% of DMSO, the remaining activity is still 733 Desulfitobacterium hafniense

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.22 Desulfitobacterium hafniense
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.22 Poros column chromatography and Q-FF column chromatography Desulfitobacterium hafniense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.22 4-nitrophenyl sulfate + 1-butanol 10% conversion in 7 days Desulfitobacterium hafniense 4-nitrophenol + butyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + 1-naphthol 50% activity compared to phenol Desulfitobacterium hafniense 4-nitrophenol + 1-naphththyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + 1-octanol 10% conversion in 7 days Desulfitobacterium hafniense 4-nitrophenol + octyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + 1-pentanol 10% conversion in 7 days Desulfitobacterium hafniense 4-nitrophenol + pentyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + 1-phenylethanol 2-10% conversion in 7 days Desulfitobacterium hafniense 4-nitrophenol + 1-phenylethyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + 17beta-estradiol after 24 h about 80% of the 17beta-estradiol is converted Desulfitobacterium hafniense 4-nitrophenol + ?
-
 ?
2.8.2.22 4-nitrophenyl sulfate + 2-aminophenol 136% activity compared to phenol Desulfitobacterium hafniense 4-nitrophenol + 2-aminophenyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + 2-butanol 2-10% conversion in 7 days Desulfitobacterium hafniense 4-nitrophenol + 2-butyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + 2-naphthol 86% activity compared to phenol Desulfitobacterium hafniense 4-nitrophenol + 2-naphthyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + 2-octanol 2-10% conversion in 7 days Desulfitobacterium hafniense 4-nitrophenol + 2-octyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + 2-phenylethanol 50% conversion in 7 days Desulfitobacterium hafniense 4-nitrophenol + 2-phenylethyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + 3,5-dimethoxyphenol 33% activity compared to phenol Desulfitobacterium hafniense 4-nitrophenol + 3,5-dimethoxyphenyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + 3-chlorophenol 138% activity compared to phenol Desulfitobacterium hafniense 4-nitrophenol + 3-chlorophenyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + 4-aminophenol 102% activity compared to phenol Desulfitobacterium hafniense 4-nitrophenol + 4-aminophenyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + 4-chlorophenol 195% activity compared to phenol Desulfitobacterium hafniense 4-nitrophenol + 4-chlorophenyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + 4-cresol 200% activity compared to phenol Desulfitobacterium hafniense 4-nitrophenol + 4-cresol sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + 4-hydroxybenzoic acid 0.7% activity compared to phenol Desulfitobacterium hafniense ?
-
 ?
2.8.2.22 4-nitrophenyl sulfate + 4-hydroxyindole 88% activity compared to phenol Desulfitobacterium hafniense 4-nitrophenol + indol-4-yl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + 5-hydroxyindole 74% activity compared to phenol Desulfitobacterium hafniense 4-nitrophenol + indol-5-yl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + benzyl alcohol 10% conversion in 7 days Desulfitobacterium hafniense 4-nitrophenol + benzyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + cyclohexanol 10% conversion in 7 days Desulfitobacterium hafniense 4-nitrophenol + cyclohexyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + D-glucose 2-10% conversion in 7 days Desulfitobacterium hafniense ?
-
 ?
2.8.2.22 4-nitrophenyl sulfate + dihydroxyacetone 2-10% conversion in 7 days Desulfitobacterium hafniense 4-nitrophenol + ?
-
 ?
2.8.2.22 4-nitrophenyl sulfate + dopamine 540% activity compared to phenol Desulfitobacterium hafniense 4-nitrophenol + ?
-
 ?
2.8.2.22 4-nitrophenyl sulfate + estrone after 24 h 90% of the estrone is sulfated Desulfitobacterium hafniense 4-nitrophenol + estrone 3-sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + furfuryl alcohol 10% conversion in 7 days Desulfitobacterium hafniense 4-nitrophenol + furfuryl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + glycerol 10% conversion in 7 days Desulfitobacterium hafniense 4-nitrophenol + ?
-
 ?
2.8.2.22 4-nitrophenyl sulfate + Leu-enkephalin within 48 h 91% of Leu-enkephalin is sulfated which reaches 98% after 6 days of incubation Desulfitobacterium hafniense 4-nitrophenol + Leu-enkephalin sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + N-hydroxysuccinimide 2-10% conversion in 7 days Desulfitobacterium hafniense ?
-
 ?
2.8.2.22 4-nitrophenyl sulfate + octyl glucoside 2-10% conversion in 7 days Desulfitobacterium hafniense ?
-
 ?
2.8.2.22 4-nitrophenyl sulfate + phenol 100% activity Desulfitobacterium hafniense 4-nitrophenol + phenyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + resorcinol 70% activity compared to phenol Desulfitobacterium hafniense 4-nitrophenol + resorcinyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + tyramine 60% activity compared to phenol Desulfitobacterium hafniense 4-nitrophenol + 4-(2-aminoethyl)phenyl sulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.22 ? x * 70000, SDS-PAGE Desulfitobacterium hafniense
2.8.2.22 ? x * 71400, calculated from amino acid sequence Desulfitobacterium hafniense

Synonyms

EC Number Synonyms Comment Organism
2.8.2.22 arylsulfate sulfotransferase
-
 Desulfitobacterium hafniense
2.8.2.22 AST
-
 Desulfitobacterium hafniense

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.8.2.22 47
-
 the melting temperature is at 47°C Desulfitobacterium hafniense

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.22 9.6
-
-
 Desulfitobacterium hafniense

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.8.2.22 9 10
-
 Desulfitobacterium hafniense