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Literature summary extracted from

  • Sircar, D.; Mukherjee, C.; Beuerle, T.; Beerhues, L.; Mitra, A.
    Characterization of p-hydroxybenzaldehyde dehydrogenase, the final enzyme of p-hydroxybenzoic acid biosynthesis in hairy roots of Daucus carota (2011), Acta Physiol. Plant., 33, 2019-2024.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.64 DTT maximum 12% increase of activity observed with 0.1 mM Daucus carota
1.2.1.64 additional information the activity is not strictly dependent on the presence of a reducing agent, such as DTT in the reaction mixture Daucus carota

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.64 0.0548
-
 4-hydroxybenzaldehyde apparent Km-value, pH 7.5 and 35°C Daucus carota
1.2.1.64 0.0744
-
 NAD+ apparent Km-value, pH 7.5 and 35°C Daucus carota

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.64 Ca2+ 1 mM chloride salt, 87.2% relative activity compared to control Daucus carota
1.2.1.64 Co2+ 1 mM chloride salt, 67.5% relative activity compared to control Daucus carota
1.2.1.64 Cu2+ 1 mM chloride salt, 34.7% relative activity compared to control Daucus carota
1.2.1.64 Fe2+ 1 mM chloride salt, 93.6% relative activity compared to control Daucus carota
1.2.1.64 Mg2+ 1 mM chloride salt, 88% relative activity compared to control Daucus carota
1.2.1.64 Mn2+ 1 mM chloride salt, 80.6% relative activity compared to control Daucus carota
1.2.1.64 Zn2+ 1 mM chloride salt, 49.9% relative activity compared to control Daucus carota

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.64 Daucus carota
-
 hairy root cultures
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.64 0.0072
-
 constant activity in non-treated root lines, pH 7.5 and 35°C Daucus carota
1.2.1.64 0.0072
-
 up to 6 h of methyl jasmonate treatment, pH 7.5 and 35°C Daucus carota
1.2.1.64 0.0384
-
 peak after 24 h of methyl jasmonate treatment, pH 7.5 and 35°C Daucus carota

Storage Stability

EC Number Storage Stability Organism
1.2.1.64 a loss of 4-hydroxybenzaldehyde dehydrogenase activity to the range of 75%, 35% and 20% of the optimum value is observed when cell-free extracts are frozen in liquid nitrogen and stored at +4, -20 and -80°C, for 24 h, respectively Daucus carota

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.64 2-hydroxybenzaldehyde + NAD+ + H2O 12.3% relative activity compared to 4-hydroxybenzaldehyde conversion Daucus carota 2-hydroxybenzoate + NADH + 2 H+
-
 ?
1.2.1.64 2-methoxybenzaldehyde + NAD+ + H2O 4.8% relative activity compared to 4-hydroxybenzaldehyde conversion, 2-methoxybenzaldehyde i.e. o-anisaldehyde Daucus carota 2-methoxybenzoate + NADH + 2 H+ 2-methoxybenzoate i.e. o-anisic acid ?
1.2.1.64 3-hydroxybenzaldehyde + NAD+ + H2O 27.4% relative activity compared to 4-hydroxybenzaldehyde conversion Daucus carota 3-hydroxybenzoate + NADH + 2 H+
-
 ?
1.2.1.64 4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O 1% relative activity compared to 4-hydroxybenzaldehyde conversion, 4-hydroxy-3-methoxybenzaldehyde i.e. vanillin Daucus carota 4-hydroxy-3-methoxybenzoate + NADH + 2 H+ 4-hydroxy-3-methoxybenzoate i.e. vanillic acid ?
1.2.1.64 4-hydroxybenzaldehyde + FAD + H2O 17.6% relative activity compared to reaction with NAD+ Daucus carota 4-hydroxybenzoate + FADH2 + ?
-
 ?
1.2.1.64 4-hydroxybenzaldehyde + FMN + H2O 11.5% relative activity compared to reaction with NAD+ Daucus carota 4-hydroxybenzoate + FMNH2 + ?
-
 ?
1.2.1.64 4-hydroxybenzaldehyde + NAD+ + H2O preferred substrate, rapid increase in 4-hydroxybenzoic acid accumulation in Daucus carota hairy root cultures detectable between 15 and 25 days after subculture preceded by an increase in 4-hydroxybenzaldehyde dehydrogenase activity Daucus carota 4-hydroxybenzoate + NADH + 2 H+
-
 ir
1.2.1.64 4-hydroxybenzaldehyde + NADP+ + H2O 70.4% relative activity compared to reaction with NAD+ Daucus carota 4-hydroxybenzoate + NADPH + 2 H+
-
 ?
1.2.1.64 benzaldehyde + NAD+ + H2O 5.8% relative activity compared to 4-hydroxybenzaldehyde conversion Daucus carota benzoate + NADH + 2 H+
-
 ?
1.2.1.64 cinnamaldehyde + NAD+ + H2O 6.13% relative activity compared to 4-hydroxybenzaldehyde conversion Daucus carota cinnamic acid + NADH + 2 H+
-
 ?
1.2.1.64 additional information no activity with 3,4-dihydroxybenzaldehyde i.e. protocatechuic aldehyde and 3,4,5-trihydroxybenzaldehyde Daucus carota ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.64 4-hydroxybenzaldehyde dehydrogenase
-
 Daucus carota
1.2.1.64 HBD
-
 Daucus carota

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.64 35 40
-
 Daucus carota

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.64 7.5
-
-
 Daucus carota

Expression

EC Number Organism Comment Expression
1.2.1.64 Daucus carota hairy root cultures of Daucus carota respond to methyl jasmonate treatment with enhanced accumulation of 4-hydroxybenzoic acid up

General Information

EC Number General Information Comment Organism
1.2.1.64 malfunction 4-hydroxybenzaldehyde dehydrogenase is the final enzyme of 4-hydroxybenzoic acid biosynthesis Daucus carota