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Literature summary extracted from

  • Lu, X.; Yi, Q.; Zhang, G.; Zhu, X.; Zhou, H.; Dong, H.
    Crystallization and preliminary X-ray analysis of an alanine dehydrogenase from Bacillus megaterium WSH-002 (2013), Acta Crystallogr. Sect. F, 69, 934-936.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.1 gene ald, expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Priestia megaterium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.1.1 purified recombinant His6-tagged enzyme, hanging drop vapour diffusion method, 15 mg/ml protein in 20 mM Tris-HCl, pH 8.0, mixed with 0.1 M HEPES, pH 8.0, 12% w/v PEG 8000, and 8% v/v ethylene glycol, 4°C, method optimization, X-ray diffraction structure determination and analysis at 2.35 A resolution Priestia megaterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.1 42000
-
 x * 42000, recombinant His6-tagged enzme, SDS-PAGE Priestia megaterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.1 L-alanine + H2O + NAD+ Priestia megaterium
-
 pyruvate + NH3 + NADH + H+
-
 r
1.4.1.1 L-alanine + H2O + NAD+ Priestia megaterium WSH-002
-
 pyruvate + NH3 + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.1 Priestia megaterium A0A0L1M9S8 gene ald
-
1.4.1.1 Priestia megaterium WSH-002 A0A0L1M9S8 gene ald
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.1 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.1 L-alanine + H2O + NAD+
-
 Priestia megaterium pyruvate + NH3 + NADH + H+
-
 r
1.4.1.1 L-alanine + H2O + NAD+
-
 Priestia megaterium WSH-002 pyruvate + NH3 + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.1 ? x * 42000, recombinant His6-tagged enzme, SDS-PAGE Priestia megaterium

Synonyms

EC Number Synonyms Comment Organism
1.4.1.1 L-AlaDH
-
 Priestia megaterium

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.1 NAD+
-
 Priestia megaterium
1.4.1.1 NADH
-
 Priestia megaterium