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Literature summary extracted from

  • Coitinho, J.B.; Costa, D.M.; Guimaraes, S.L.; de Goes, A.M.; Nagem, R.A.
    Expression, purification and preliminary crystallographic studies of NahF, a salicylaldehyde dehydrogenase from Pseudomonas putida G7 involved in naphthalene degradation (2012), Acta Crystallogr. Sect. F, 68, 93-97.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.65 nahF gene subcloned into the pET28a(TEV) vector and the recombinant protein with 6xHis tag at the N-terminaus overexpressed in Escherichia coli BL21 Arctic Express (DE3) cells Pseudomonas putida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.65 crystals of recombinant 6xHis-NahF obtained at 291 K and diffracted to 2.42 A resolution Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.65 125000
-
-
 Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.65 Pseudomonas putida
-
-
-
1.2.1.65 Pseudomonas putida G7
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.65 by Ni2+ affinity chromatography followed by gel filtration Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.65 additional information nah operon in Pseudomonas putida, which is present on the 83 kb metabolic plasmid NAH7 includes nahF gene coding for salicylaldehyde dehydrogenase Pseudomonas putida ?
-
 ?
1.2.1.65 additional information nah operon in Pseudomonas putida, which is present on the 83 kb metabolic plasmid NAH7 includes nahF gene coding for salicylaldehyde dehydrogenase Pseudomonas putida G7 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.65 dimer suggested by the elution profile in the SEC chromatogram Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.2.1.65 NahF
-
 Pseudomonas putida

General Information

EC Number General Information Comment Organism
1.2.1.65 metabolism NahF catalyzes the last reaction in the conversion of naphthalene to salicylate Pseudomonas putida