Literature summary extracted from
Zhou, H.; Sun, L.; Li, J.; Xu, C.; Yu, F.; Liu, Y.; Ji, C.; He, J.
The crystal structure of human GDP-L-fucose synthase (2013), Acta Biochim. Biophys. Sin. (Shanghai), 45, 720-725.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.1.1.271 |
gene fx, expression of the N-terminally His6-tagged enzyme in Escherichia coli |
Homo sapiens |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
1.1.1.271 |
purified recombinant enzyme, hanging drop vapor diffusion method, 5 mg/ml protein in 20 mM Tris-HCl, pH 8.0, and 100 mM NaCl, is mixed with well solution containing 100 mM Tris, pH 8.0, 30% PEG monomethyl ether 2000, X-ray diffraction structure determination and analysis at 2.37 A resolution, molecular replacement |
Homo sapiens |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.1.1.271 |
Homo sapiens |
- |
gene fx |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.1.1.271 |
recombinant N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration |
Homo sapiens |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
1.1.1.271 |
erythrocyte |
- |
Homo sapiens |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.1.1.271 |
GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADPH + H+ |
the enzyme synthesizes GDP-L-fucose from its substrate GDP-4-keto-6-deoxy-D-mannose. The reaction involves epimerization at both C-3 and C-5 followed by an NADPH-dependent reduction of the carbonyl at C-4 |
Homo sapiens |
GDP-beta-L-fucose + NADP+ |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.1.1.271 |
tetramer |
two homodimers, each subunit consists of two domains, a Rossmann-fold NADPH-binding motif and a carboxyl terminal domain, quaternary structure and intermolecular contacts, overview |
Homo sapiens |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.1.1.271 |
FX protein |
- |
Homo sapiens |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.1.1.271 |
NADPH |
dependent on, binding site structure, overview |
Homo sapiens |
|