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Literature summary extracted from

  • Rudolf, J.D.; Poulter, C.D.
    Tyrosine O-prenyltransferase SirD catalyzes S-, C-, and N-prenylations on tyrosine and tryptophan derivatives (2013), ACS Chem. Biol., 8, 2707-2714.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.122 0.3
-
 L-tyrosine pH 8.0, 30°C Leptosphaeria maculans
2.5.1.122 0.37
-
 L-tryptophan pH 8.0, 30°C Leptosphaeria maculans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.122 dimethylallyl diphosphate + L-tyrosine Leptosphaeria maculans first committed step in the biosynthesis of the phytotoxin sirodesmin PL diphosphate + 4-O-dimethylallyl-L-tyrosine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.122 Leptosphaeria maculans Q6Q874
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.122
-
 Leptosphaeria maculans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.122 dimethylallyl diphosphate + L-tryptophan reverse prenylation Leptosphaeria maculans diphosphate + 1-(2-methylbut-3-en-2-yl)-L-tryptophan
-
 ?
2.5.1.122 dimethylallyl diphosphate + L-tryptophan catalytic efficiency for L-tryptophan is 6% compared to catalytic efficiency for L-tyrosine Leptosphaeria maculans diphosphate + 7-dimethylallyltryptophan
-
 ?
2.5.1.122 dimethylallyl diphosphate + L-tyrosine
-
 Leptosphaeria maculans diphosphate + 4-O-dimethylallyl-L-tyrosine
-
 ?
2.5.1.122 dimethylallyl diphosphate + L-tyrosine first committed step in the biosynthesis of the phytotoxin sirodesmin PL Leptosphaeria maculans diphosphate + 4-O-dimethylallyl-L-tyrosine
-
 ?
2.5.1.122 additional information the catalytic erfficiency with 4-mercapto-L-phenylalanine, 4-methoxy-L-tryptophan and 7-methyl-L-tryptophan is less than 1% of the reaction with L-tyrosine Leptosphaeria maculans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.122 SirD
-
 Leptosphaeria maculans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.122 30
-
 assay at Leptosphaeria maculans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.122 0.095
-
 L-tryptophan pH 8.0, 30°C Leptosphaeria maculans
2.5.1.122 1.3
-
 L-tyrosine pH 8.0, 30°C Leptosphaeria maculans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.122 8
-
 assay at Leptosphaeria maculans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.122 0.26
-
 L-tryptophan pH 8.0, 30°C Leptosphaeria maculans
2.5.1.122 4.3
-
 L-tyrosine pH 8.0, 30°C Leptosphaeria maculans