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Literature summary extracted from

  • Ishikura, N.; Yang, Z.
    UDO-xylose:flavonol 3-O-xylosyltransferase from young leaves of Euonymus alatus f. ciliato-dentatus (1991), Z. Naturforsch. C, 46, 1003-1010.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.56 2-mercaptoethanol strong stimulation even in the presence of Mg2+ Euonymus alatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.56 Ca2+ 10 mM, 75% inhibition Euonymus alatus
2.4.2.56 Co2+ 1 mM, 96% inhibition Euonymus alatus
2.4.2.56 Cu2+ 1 mM, 99% inhibition Euonymus alatus
2.4.2.56 iodoacetamide 1 mM, 44% inhibition Euonymus alatus
2.4.2.56 Mg2+ 20 mM, 77% inhibition Euonymus alatus
2.4.2.56 Mn2+ 10 mM, 98% inhibition Euonymus alatus
2.4.2.56 Phenylmercuriacetate 1 mM, 98% inhibition Euonymus alatus
2.4.2.56 Zn2+ 1 mM, 99% inhibition Euonymus alatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.56 0.00083
-
 kaempferol pH 7.5, 30°C Euonymus alatus
2.4.2.56 0.00119
-
 quercetin pH 7.5, 30°C Euonymus alatus
2.4.2.56 0.025
-
 UDP-alpha-D-xylose pH 7.5, 30°C, cosubstrate: kaempferol Euonymus alatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.56 48000
-
 gel filtration Euonymus alatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.56 UDP-alpha-D-xylose + kaempferol Euonymus alatus 3-O-xylosylation of flavonols UDP + kaempferol 3-O-beta-D-xyloside
-
 ?
2.4.2.56 UDP-alpha-D-xylose + kaempferol Euonymus alatus Thunb. Siebold f. ciliatodentatus 3-O-xylosylation of flavonols UDP + kaempferol 3-O-beta-D-xyloside
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.56 Euonymus alatus
-
-
-
2.4.2.56 Euonymus alatus Thunb. Siebold f. ciliatodentatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.56 partial Euonymus alatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.56 leaf young Euonymus alatus
-

Storage Stability

EC Number Storage Stability Organism
2.4.2.56 -20°C, 20 mM Tris-HCl buffer, pH 7.5, 14 mM 2-mercaptoethanol and 10% glycerol, 11% loss of activity after 2 days, 55% loss after 8 days, 84% loss after 40 days Euonymus alatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.56 UDP-alpha-D-xylose + 3,3',4',7-tetrahydroxy-5'-methoxyflavone 20% of the activity compared to kaempferol Euonymus alatus UDP + 3,3',4',7-tetrahydroxy-5'-methoxyflavone 3-O-beta-D-xyloside
-
 ?
2.4.2.56 UDP-alpha-D-xylose + 3,3',4',7-tetrahydroxy-5'-methoxyflavone 20% of the activity compared to kaempferol Euonymus alatus Thunb. Siebold f. ciliatodentatus UDP + 3,3',4',7-tetrahydroxy-5'-methoxyflavone 3-O-beta-D-xyloside
-
 ?
2.4.2.56 UDP-alpha-D-xylose + 3,4',7-trihydroxyflavone 32% of the activity compared to kaempferol Euonymus alatus UDP + 3,4',7-trihydroxyflavone 3-O-beta-D-xyloside
-
 ?
2.4.2.56 UDP-alpha-D-xylose + 3,4',7-trihydroxyflavone 32% of the activity compared to kaempferol Euonymus alatus Thunb. Siebold f. ciliatodentatus UDP + 3,4',7-trihydroxyflavone 3-O-beta-D-xyloside
-
 ?
2.4.2.56 UDP-alpha-D-xylose + 3,5,7-trihydroxy-4'-methoxyflavone 45% of the activity compared to kaempferol Euonymus alatus UDP + 3,5,7-trihydroxy-4'-methoxyflavone 3-O-beta-D-xyloside
-
 ?
2.4.2.56 UDP-alpha-D-xylose + 3,5,7-trihydroxy-4'-methoxyflavone 45% of the activity compared to kaempferol Euonymus alatus Thunb. Siebold f. ciliatodentatus UDP + 3,5,7-trihydroxy-4'-methoxyflavone 3-O-beta-D-xyloside
-
 ?
2.4.2.56 UDP-alpha-D-xylose + 3-hydroxy-4',5,7-trimethoxyflavone 15% of the activity compared to kaempferol Euonymus alatus UDP + 3-hydroxy-4',5,7-trimethoxyflavone 3-O-beta-D-xyloside
-
 ?
2.4.2.56 UDP-alpha-D-xylose + 4',5,7-trihydroxyflavan-3-ol 25% of the activity compared to kaempferol Euonymus alatus UDP + 3-O-beta-D-xylopyranosyl-4',5,7-trihydroxyflavan-3-ol
-
 ?
2.4.2.56 UDP-alpha-D-xylose + fisetin 62% of the activity compared to kaempferol Euonymus alatus UDP + fisetin 3-O-beta-D-xyloside
-
 ?
2.4.2.56 UDP-alpha-D-xylose + isorhamnetin 85% of the activity compared to kaempferol Euonymus alatus UDP + isorhamnetin 3-O-beta-D-xyloside
-
 ?
2.4.2.56 UDP-alpha-D-xylose + kaempferol best substrate Euonymus alatus UDP + kaempferol 3-O-beta-D-xyloside
-
 ?
2.4.2.56 UDP-alpha-D-xylose + kaempferol 3-O-xylosylation of flavonols Euonymus alatus UDP + kaempferol 3-O-beta-D-xyloside
-
 ?
2.4.2.56 UDP-alpha-D-xylose + kaempferol best substrate Euonymus alatus Thunb. Siebold f. ciliatodentatus UDP + kaempferol 3-O-beta-D-xyloside
-
 ?
2.4.2.56 UDP-alpha-D-xylose + kaempferol 3-O-xylosylation of flavonols Euonymus alatus Thunb. Siebold f. ciliatodentatus UDP + kaempferol 3-O-beta-D-xyloside
-
 ?
2.4.2.56 UDP-alpha-D-xylose + myricetin 48% of the activity compared to kaempferol Euonymus alatus UDP + myricetin 3-O-beta-D-xyloside
-
 ?
2.4.2.56 UDP-alpha-D-xylose + quercetin 76% of the activity compared to kaempferol Euonymus alatus UDP + quercetin 3-O-beta-D-xyloside
-
 ?
2.4.2.56 UDP-alpha-D-xylose + rhamnetin 67% of the activity compared to kaempferol Euonymus alatus UDP + rhamnetin 3-O-beta-D-xyloside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.56 F3XT
-
 Euonymus alatus
2.4.2.56 flavonol 3-O-xylosyltransferase
-
 Euonymus alatus
2.4.2.56 UDP-D-xylose:flavonol 3-O-xylosyltransferase
-
 Euonymus alatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.56 7
-
 Tris-HCl buffer Euonymus alatus
2.4.2.56 7.5
-
 imidazole-HCl buffer Euonymus alatus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.2.56 Euonymus alatus isoelectric focusing
-
 6.1