Literature summary extracted from

Tian, Y.; Xu, J.; Han, J.; Zhao, W.; Fu, X.; Peng, R.; Yao, Q.
Complementary screening, identification and application of a novel class II 5-enopyruvylshikimate-3-phosphate synthase from Bacillus cereus (2012), World J. Microbiol. Biotechnol., 29, 549-557.

Application

EC Number	Application	Comment	Organism
2.5.1.19	agriculture	transgenic Arabidopsis thaliana plants expressing the enzyme from Bacillus cereus germinate well in presence of 1 mM glyphosate, while control plants do not germinate at 0.2 mM	Bacillus cereus

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.5.1.19	expression in Escherichia coli	Bacillus cereus

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.5.1.19	glyphosate	-	Bacillus cereus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.5.1.19	0.195	-	phosphoenolpyruvate	pH 7.0, 28°C	Bacillus cereus
2.5.1.19	0.304	-	3-phosphoshikimate	pH 7.0, 28°C	Bacillus cereus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.5.1.19	43350	-	x * 43350, calculated	Bacillus cereus

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.5.1.19	Bacillus cereus	F5A7Q0	class II enzyme	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.5.1.19	phosphoenolpyruvate + 3-phosphoshikimate	-	Bacillus cereus	phosphate + 5-enolpyruvylshikimate 3-phosphate	-	?

Subunits

EC Number	Subunits	Comment	Organism
2.5.1.19	?	x * 43350, calculated	Bacillus cereus

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.5.1.19	31.8	-	phosphoenolpyruvate	pH 7.0, 28°C	Bacillus cereus

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
2.5.1.19	0.052	-	glyphosate	pH 7.0, 28°C	Bacillus cereus

IC50 Value

EC Number	IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
2.5.1.19	0.458	-	pH 7.0, 28°C	Bacillus cereus	glyphosate

kcat/KM [mM/s]

EC Number	kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
2.5.1.19	163.1	-	phosphoenolpyruvate	pH 7.0, 28°C	Bacillus cereus

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Release 2023.1 (January 2023)