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Literature summary extracted from

  • Xie, X.; Wang, G.; Xia, J.; Chen, N.
    Characterization of a recombinant cold-adapted purine nucleoside phosphorylase and its application in ribavirin bioconversion (2011), World J. Microbiol. Biotechnol., 27, 1175-1181.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.2.1 synthesis synthesis of ribavirin by the enzyme and comparison with Escherichia coli enzyme. A cold-adapted Pseudoalteromonas enzyme shows approximately 15 degrees lower optimum temperature and 1.80fold higher catalytic efficiency at 37°C within substrate inosine than its homolog from Escherichia coli Pseudoalteromonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.39
-
 Inosine pH 7.6, 37°C Pseudoalteromonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.1 25000
-
 x * 25000, calculated and SDS-PAGE Pseudoalteromonas sp.

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Pseudoalteromonas sp. C8CPR9
-
-
2.4.2.1 Pseudoalteromonas sp. XM2107 C8CPR9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 inosine + phosphate
-
 Pseudoalteromonas sp. hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Pseudoalteromonas sp. XM2107 hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.1 ? x * 25000, calculated and SDS-PAGE Pseudoalteromonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.1 40
-
 stable up to Pseudoalteromonas sp.
2.4.2.1 50
-
 complete inactivation Pseudoalteromonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.1 84.1
-
 Inosine pH 7.6, 37°C Pseudoalteromonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.1 7.6
-
-
 Pseudoalteromonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.2.1 6 8 stable for at least 2 h at 37°C Pseudoalteromonas sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.1 215
-
 Inosine pH 7.6, 37°C Pseudoalteromonas sp.