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Literature summary extracted from

  • Buckoreelall, K.; Sun, Y.; Hobrath, J.V.; Wilson, L.; Parker, W.B.
    Identification of Rv0535 as methylthioadenosine phosphorylase from Mycobacterium tuberculosis (2012), Tuberculosis, 92, 139-147.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.28 expressed in Escherichia coli Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.28 5'-methylthio-DADMe-immucillin A
-
 Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.28 0.009
-
 5'-methylthioadenosine pH and temperature not specified in the publication, co-substrate: 50 mM phosphate Mycobacterium tuberculosis
2.4.2.28 0.26
-
 phosphate pH and temperature not specified in the publication, co-substrate: 0.2 mM 5'-methylthioadenosine Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.28 30000
-
 SDS-PAGE Mycobacterium tuberculosis
2.4.2.28 60000
-
 gel filtration Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.28 Mycobacterium tuberculosis O06401
-
-

Storage Stability

EC Number Storage Stability Organism
2.4.2.28 -20°C, stable Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.28 5'-methylthioadenosine + phosphate
-
 Mycobacterium tuberculosis adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.28 adenosine + phosphate 2.1% activity compared to activity with 5'-methylthioadenosine Mycobacterium tuberculosis adenine + D-ribose 1-phosphate
-
 ?
2.4.2.28 S-adenosyl-L-homocysteine + phosphate 0.81% activity compared to activity with 5'-methylthioadenosine Mycobacterium tuberculosis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.28 dimer 2 * 30000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.4.2.28 MTAP
-
 Mycobacterium tuberculosis
2.4.2.28 Rv0535
-
 Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.28 4
-
 4h, 40% loss of activity Mycobacterium tuberculosis
2.4.2.28 37
-
 4h, 90% loss of activity Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.28 0.26
-
 phosphate pH and temperature not specified in the publication, co-substrate: 0.2 mM 5'-methylthioadenosine Mycobacterium tuberculosis
2.4.2.28 0.4
-
 5'-methylthioadenosine pH and temperature not specified in the publication, co-substrate: 50 mM phosphate Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.28 7
-
 7-7.5 optimal pH Mycobacterium tuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.28 6.5 9 pH 6.5: about 55% of maximal activity, pH 9.0: about 55% of maximal activity Mycobacterium tuberculosis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.4.2.28 0.000025
-
 pH and temperature not specified in the publication, in the presence of 10 microM 5'-methylthioadenosine Mycobacterium tuberculosis 5'-methylthio-DADMe-immucillin A
2.4.2.28 0.000075
-
 pH and temperature not specified in the publication, in the presence of 100 microM 5'-methylthioadenosine Mycobacterium tuberculosis 5'-methylthio-DADMe-immucillin A