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Literature summary extracted from

  • Tao, S.; He, Y.; Ying, Y.; Hong, X.; Xu, X.; Zhu, F.; Lv, H.; Yan, L.
    C35T mutation could slightly decrease the activity of human alpha-(1,2)-fucosyltransferase (2012), Transfus. Clin. Biol., 19, 5-10.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.69 expressed in COS-7 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.69 C35T the mutation is a polymorphism in the Chinese population and does not affect its mRNA transcription, but can slightly decrease the activity of human alpha-(1,2)-fucosyltransferase in vitro Homo sapiens
2.4.1.69 S12F the mutation does not significantly decrease the activity of alpha-(1,2)-fucosyltransferase and affect its kinetic property Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.69 0.3984
-
GDP-L-fucose Km above 0.3984 mM, isoform FUT1 mutant C35T, in 50 mM Tris-HCl (pH 7.2), at 37°C Homo sapiens
2.4.1.69 0.3984
-
GDP-L-fucose wild type isoform FUT1, in 50 mM Tris-HCl (pH 7.2), at 37°C Homo sapiens
2.4.1.69 83.6
-
phenyl-beta-D-galactoside wild type isoform FUT1, in 50 mM Tris-HCl (pH 7.2), at 37°C Homo sapiens
2.4.1.69 125
-
phenyl-beta-D-galactoside isoform FUT1 mutant C35T, in 50 mM Tris-HCl (pH 7.2), at 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.69 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.69 GDP-L-fucose + phenyl-beta-D-galactoside
-
Homo sapiens ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.69 alpha-(1,2)-fucosyltransferase
-
Homo sapiens
2.4.1.69 FUT1 isoform Homo sapiens
2.4.1.69 FUT2 isoform Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.69 0.007
-
GDP-L-fucose wild type isoform FUT1, in 50 mM Tris-HCl (pH 7.2), at 37°C Homo sapiens
2.4.1.69 0.011
-
phenyl-beta-D-galactoside isoform FUT1 mutant C35T, in 50 mM Tris-HCl (pH 7.2), at 37°C Homo sapiens
2.4.1.69 0.011
-
phenyl-beta-D-galactoside wild type isoform FUT1, in 50 mM Tris-HCl (pH 7.2), at 37°C Homo sapiens

General Information

EC Number General Information Comment Organism
2.4.1.69 physiological function the H antigen of human blood group is synthesized by alpha-(1,2)-fucosyltransferase Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.69 0.000088
-
phenyl-beta-D-galactoside isoform FUT1 mutant C35T, in 50 mM Tris-HCl (pH 7.2), at 37°C Homo sapiens
2.4.1.69 0.000132
-
phenyl-beta-D-galactoside wild type isoform FUT1, in 50 mM Tris-HCl (pH 7.2), at 37°C Homo sapiens
2.4.1.69 0.0183
-
GDP-L-fucose wild type isoform FUT1, in 50 mM Tris-HCl (pH 7.2), at 37°C Homo sapiens