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Literature summary extracted from

  • Arai, S.; Yonezawa, Y.; Okazaki, N.; Matsumoto, F.; Tamada, T.; Tokunaga, H.; Ishibashi, M.; Blaber, M.; Tokunaga, M.; Kuroki, R.
    A structural mechanism for dimeric to tetrameric oligomer conversion in Halomonas sp. nucleoside diphosphate kinase (2012), Protein Sci., 21, 498-510.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.6 expressed in Escherichia coli BL21 Star(DE3) cells Halomonas sp. #593

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.4.6 sitting drop vapor diffusion method, crystal form 1 is a cubic shaped crystal grown from 0.2M ammonium acetate, 0.1M sodium citrate tribasic dehydrate at pH 5.6, and 30%(w/v) polyethylene glycol (PEG) 4000, while crystal form 2 is a rod-shaped crystal grown from 0.19M sodium acetate, 0.09M Tris HCl at pH 8.5, and 28%(w/v) PEG 4000 Halomonas sp. #593

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.6 E134A the mutant forms a tetramer and shows 119% specific activity compared to the wild type enzyme Halomonas sp. #593

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.6 15137
-
2 * 15137, calculated from amino acid sequence Halomonas sp. #593
2.7.4.6 32100
-
gel filtration and multiangle laser light scattering Halomonas sp. #593

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.6 Halomonas sp. #593 Q83WH5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.6 ATP-agarose column chromatography and Hi-Trap Q resin column chromatography Halomonas sp. #593

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.6 ATP + TDP
-
Halomonas sp. #593 ADP + TTP
-
?

Subunits

EC Number Subunits Comment Organism
2.7.4.6 homodimer 2 * 15137, calculated from amino acid sequence Halomonas sp. #593

Synonyms

EC Number Synonyms Comment Organism
2.7.4.6 NDK
-
Halomonas sp. #593
2.7.4.6 nucleoside diphosphate kinase
-
Halomonas sp. #593