Literature summary extracted from
Arai, S.; Yonezawa, Y.; Okazaki, N.; Matsumoto, F.; Tamada, T.; Tokunaga, H.; Ishibashi, M.; Blaber, M.; Tokunaga, M.; Kuroki, R.
A structural mechanism for dimeric to tetrameric oligomer conversion in Halomonas sp. nucleoside diphosphate kinase (2012), Protein Sci., 21, 498-510.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.7.4.6 |
expressed in Escherichia coli BL21 Star(DE3) cells |
Halomonas sp. #593 |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
2.7.4.6 |
sitting drop vapor diffusion method, crystal form 1 is a cubic shaped crystal grown from 0.2M ammonium acetate, 0.1M sodium citrate tribasic dehydrate at pH 5.6, and 30%(w/v) polyethylene glycol (PEG) 4000, while crystal form 2 is a rod-shaped crystal grown from 0.19M sodium acetate, 0.09M Tris HCl at pH 8.5, and 28%(w/v) PEG 4000 |
Halomonas sp. #593 |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.7.4.6 |
E134A |
the mutant forms a tetramer and shows 119% specific activity compared to the wild type enzyme |
Halomonas sp. #593 |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.7.4.6 |
15137 |
- |
2 * 15137, calculated from amino acid sequence |
Halomonas sp. #593 |
2.7.4.6 |
32100 |
- |
gel filtration and multiangle laser light scattering |
Halomonas sp. #593 |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.4.6 |
Halomonas sp. #593 |
Q83WH5 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.7.4.6 |
ATP-agarose column chromatography and Hi-Trap Q resin column chromatography |
Halomonas sp. #593 |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.4.6 |
ATP + TDP |
- |
Halomonas sp. #593 |
ADP + TTP |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.7.4.6 |
homodimer |
2 * 15137, calculated from amino acid sequence |
Halomonas sp. #593 |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.7.4.6 |
NDK |
- |
Halomonas sp. #593 |
2.7.4.6 |
nucleoside diphosphate kinase |
- |
Halomonas sp. #593 |