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Literature summary extracted from

  • Fucile, G.; Garcia, C.; Carlsson, J.; Sunnerhagen, M.; Christendat, D.
    Structural and biochemical investigation of two Arabidopsis shikimate kinases: the heat-inducible isoform is thermostable (2011), Protein Sci., 20, 1125-1136.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.71 gene expression patterns of isoforms, AtSK1 and AtSK2 showing a signature of regulatory subfunctionalization Arabidopsis thaliana

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.71 heat-stable isozyme AtSK2, X-ray diffraction structure determinaion and analysis at 2.35 A resolution Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.71 K138N AtSK1 mutation relative to AtSK2 predicted to confer substantial stabilizing effect Arabidopsis thaliana
2.7.1.71 K87R AtSK1 mutation relative to AtSK2 predicted to confer substantial stabilizing effect Arabidopsis thaliana
2.7.1.71 L233F AtSK1 mutation relative to AtSK2 predicted to confer substantial stabilizing effect Arabidopsis thaliana
2.7.1.71 N234K AtSK1 mutation relative to AtSK2 predicted to confer substantial stabilizing effect Arabidopsis thaliana
2.7.1.71 R120K AtSK1 mutation relative to AtSK2 predicted to confer substantial stabilizing effect Arabidopsis thaliana
2.7.1.71 S152N AtSK1 mutation relative to AtSK2 predicted to confer substantial stabilizing effect Arabidopsis thaliana
2.7.1.71 S251N AtSK1 mutation relative to AtSK2 predicted to confer substantial stabilizing effect Arabidopsis thaliana
2.7.1.71 T230S AtSK1 mutation relative to AtSK2 predicted to confer substantial stabilizing effect Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.71 Mg2+ required, Mg2+ coordinating residues are Thr112 and Asp128 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.71 ATP + shikimate Arabidopsis thaliana
-
 ADP + 3-phosphoshikimate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.71 Arabidopsis thaliana Q8GY88 two isozymes, AtSK1 and AtSK2, AatSK1 is heat-inducible
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.71 ATP + shikimate
-
 Arabidopsis thaliana ADP + 3-phosphoshikimate
-
 ?
2.7.1.71 additional information AtSK2 shikimate binding residues include Gly177, Gly178, Gly179, and Asp130 of the Walker B motif and Phe146, Phe154, Glu158, Arg155, and Arg243 Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.71 More AtSK2 adopts an alpha-beta-alpha fold with a central sheet of five parallel beta-strands flanked by two layers of alpha-helices. The AtSK2 structure can be divided into four domains: the reduced CORE domain comprising the central beta-sheet and flanking alpha-helices, the nucleotide binding domain, which includes the phosphate binding loop (P-loop/Walker-A motif), the disordered LID domain, which contains catalytic and substrate binding residues, and the extended shikimate binding domain, which includes a modified SK-type Walker B motif and several other substrate binding residues. Computational analysis of isozyme AtSK2 structure, overview Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.7.1.71 AtSK1
-
 Arabidopsis thaliana
2.7.1.71 AtSK2
-
 Arabidopsis thaliana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.71 37
-
 isozyme AtSK2 is highly unstable and becomes inactivated at 37°C, whereas the heat-induced isoform, AtSK1, is thermostable and fully active at 37°C Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.71 ATP
-
 Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
2.7.1.71 Arabidopsis thaliana expression of plant shikimate kinase is induced under specific conditions of environmental stress and developmental requirements in an isoform-specific manner up

General Information

EC Number General Information Comment Organism
2.7.1.71 evolution AtSK2 belongs to the nucleoside monophosphate kinase family Arabidopsis thaliana
2.7.1.71 metabolism shikimate kinase catalyzes an intermediate step in the shikimate pathway to aromatic amino acid biosynthesis Arabidopsis thaliana
2.7.1.71 additional information mechanism of thermal regulation, computational analysis of AtSK1 and AtSK2 structural variation, overview Arabidopsis thaliana