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Literature summary extracted from

  • Sun, X.F.; Li, Z.X.
    In silico and in vitro analyses identified three amino acid residues critical to the catalysis of two aphid farnesyl diphosphate synthase (2012), Protein J., 31, 417-424.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.10 expressed in Escherichia coli BL21(DE3) cells Rhopalosiphum padi

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.10 K266I the mutation abolishes the activities of isoform FPPS1 Rhopalosiphum padi
2.5.1.10 K266I the mutation abolishes the activities of isoform FPPS2 Rhopalosiphum padi
2.5.1.10 R120G the mutation abolishes the activities of isoform FPPS1 Rhopalosiphum padi
2.5.1.10 R120G the mutation abolishes the activities of isoform FPPS2 Rhopalosiphum padi
2.5.1.10 R121G the mutation abolishes the activities of isoform FPPS1 Rhopalosiphum padi
2.5.1.10 R121G the mutation abolishes the activities of isoform FPPS2 Rhopalosiphum padi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.10 45500
-
 2 * 45500, isoform FPPS1, calculated from amino acid sequence Rhopalosiphum padi
2.5.1.10 45900
-
 2 * 45900, isoform FPPS2, calculated from amino acid sequence Rhopalosiphum padi

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.10 Rhopalosiphum padi G0YZR3 isoform FPPS1; bird cherry-oat aphid
-
2.5.1.10 Rhopalosiphum padi G0YZR4 isoform FPPS2; bird cherry-oat aphid
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.10 TALON resin Co2+ metal affinity column chromatography Rhopalosiphum padi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.10 dimethylallyl diphosphate + 2 isopentenyl diphosphate
-
 Rhopalosiphum padi 2 diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.10 homodimer 2 * 45500, isoform FPPS1, calculated from amino acid sequence Rhopalosiphum padi
2.5.1.10 homodimer 2 * 45900, isoform FPPS2, calculated from amino acid sequence Rhopalosiphum padi

Synonyms

EC Number Synonyms Comment Organism
2.5.1.10 farnesyl diphosphate synthase 1
-
 Rhopalosiphum padi
2.5.1.10 farnesyl diphosphate synthase 2
-
 Rhopalosiphum padi
2.5.1.10 FPPS1 isoform Rhopalosiphum padi
2.5.1.10 FPPS2 isoform Rhopalosiphum padi

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.5.1.10 Rhopalosiphum padi isoform FPPS2, calculated from amino acid sequence
-
 6.6
2.5.1.10 Rhopalosiphum padi isoform FPPS1, calculated from amino acid sequence
-
 7.5