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Literature summary extracted from

  • Hari Prasad, O.; Nanda Kumar, Y.; Reddy, O.V.; Chaudhary, A.; Sarma, P.V.
    Cloning, expression, purification and characterization of UMP kinase from Staphylococcus aureus (2012), Protein J., 31, 345-352.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.22 expressed in Escherichia coli DH5alpha cells Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.22 GTP when 0.5 M GTP is added in the reaction mixture the affinity of the enzyme towards its UMP substrate decreases almost by 3folds. GTP increases the affinity of ATP towards the enzyme Staphylococcus aureus
2.7.4.22 UTP potent competitive inhibitor Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.22 0.00276
-
 UMP purified enzyme, in 50 mM Tris-HCl pH 7.4, at 30°C Staphylococcus aureus
2.7.4.22 0.00281
-
 UMP crude cytosolic extract, in 50 mM Tris-HCl pH 7.4, at 30°C Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.22 cytosol
-
 Staphylococcus aureus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.22 25000
-
 6 * 25000, SDS-PAGE Staphylococcus aureus
2.7.4.22 150000
-
 gel filtration Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.22 Staphylococcus aureus B8XPY6
-
-
2.7.4.22 Staphylococcus aureus ATCC 12600 B8XPY6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.22 ammonium sulfate precipitation, DEAE cellulose column chromatography, Sephadex G-200 gel filtration, and nickel metal chelate affinity chromatography Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.22 ATP + UMP
-
 Staphylococcus aureus ADP + UDP
-
 ?
2.7.4.22 ATP + UMP
-
 Staphylococcus aureus ATCC 12600 ADP + UDP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.22 homohexamer 6 * 25000, SDS-PAGE Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
2.7.4.22 UMPK
-
 Staphylococcus aureus
2.7.4.22 uridine monophosphate kinase
-
 Staphylococcus aureus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.22 0.0009
-
 UTP in 50 mM Tris-HCl pH 7.4, at 30°C Staphylococcus aureus