EC Number | Cloned (Comment) | Organism |
---|---|---|
1.17.1.8 | expressed in Escherichia coli BL21(DE3) cells | Staphylococcus aureus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.17.1.8 | 0.006 | - |
NADH | recombinant enzyme, at pH 8.0 and 30°C | Staphylococcus aureus | |
1.17.1.8 | 0.022 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | recombinant enzyme, at pH 8.0 and 30°C | Staphylococcus aureus | |
1.17.1.8 | 0.026 | - |
NADH | native enzyme, at pH 8.0 and 30°C | Staphylococcus aureus | |
1.17.1.8 | 0.039 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | native enzyme, at pH 8.0 and 30°C | Staphylococcus aureus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.17.1.8 | 27622 | - |
4 * 27622, calculated from amino acid sequence | Staphylococcus aureus |
1.17.1.8 | 27622 | - |
4 * 27622, ESI-TOF mass spectrometry | Staphylococcus aureus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.17.1.8 | Staphylococcus aureus | - |
- |
- |
1.17.1.8 | Staphylococcus aureus MRSA | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.17.1.8 | Ni2+ affinity column chromatography and Superose 12 gel filtration | Staphylococcus aureus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.17.1.8 | additional information | - |
at pH 8.0 and 30°C, the crude recombinant enzyme and enzyme after 2.9fold purification show specific activities of 5.51 and 16 units/mg, respectively | Staphylococcus aureus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.1.8 | (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADH + H+ | - |
Staphylococcus aureus | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD+ + H2O | - |
ir | |
1.17.1.8 | (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADH + H+ | - |
Staphylococcus aureus MRSA | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD+ + H2O | - |
ir | |
1.17.1.8 | (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADPH + H+ | - |
Staphylococcus aureus | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NADP+ + H2O | - |
ir | |
1.17.1.8 | (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADPH + H+ | - |
Staphylococcus aureus MRSA | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NADP+ + H2O | - |
ir |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.17.1.8 | homotetramer | 4 * 27622, calculated from amino acid sequence | Staphylococcus aureus |
1.17.1.8 | homotetramer | 4 * 27622, ESI-TOF mass spectrometry | Staphylococcus aureus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.17.1.8 | DapB | - |
Staphylococcus aureus |
1.17.1.8 | DHDPR | - |
Staphylococcus aureus |
1.17.1.8 | dihydrodipicolinate reductase | - |
Staphylococcus aureus |
1.17.1.8 | EC 1.3.1.26 | formerly | Staphylococcus aureus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.17.1.8 | 65 | - |
both the native and His-tagged recombinant enzymes show an apparent melting temperature of approximately 65°C | Staphylococcus aureus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.17.1.8 | 20 | - |
NADH | native enzyme, at pH 8.0 and 30°C | Staphylococcus aureus | |
1.17.1.8 | 20 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | native enzyme, at pH 8.0 and 30°C | Staphylococcus aureus | |
1.17.1.8 | 21 | - |
NADH | recombinant enzyme, at pH 8.0 and 30°C | Staphylococcus aureus | |
1.17.1.8 | 21 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | recombinant enzyme, at pH 8.0 and 30°C | Staphylococcus aureus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.17.1.8 | NADH | - |
Staphylococcus aureus | |
1.17.1.8 | NADPH | - |
Staphylococcus aureus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.17.1.8 | metabolism | the enzyme catalyzes the second step of the lysine biosynthesis pathway | Staphylococcus aureus |