EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.54 | expressed in soluble form by co-expression with chaperonins GroEL/GroES in Escherichia coli | Actinosynnema pretiosum subsp. auranticum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.54 | EDTA | 2 mM, complete inactivation within 10 min | Actinosynnema pretiosum subsp. auranticum | |
2.5.1.54 | additional information | substrate D-erythrose 4-phosphate is not inhibitory | Actinosynnema pretiosum subsp. auranticum | |
2.5.1.54 | phosphoenolpyruvate | pronounced substrate inhibition above 1 mM | Actinosynnema pretiosum subsp. auranticum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.54 | 0.09 | - |
phosphoenolpyruvate | pH 7.5, 30°C | Actinosynnema pretiosum subsp. auranticum | |
2.5.1.54 | 0.43 | - |
D-erythrose 4-phosphate | pH 7.5, 30°C | Actinosynnema pretiosum subsp. auranticum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.54 | Ca2+ | 0.1 mM, up to 500% of initial activity | Actinosynnema pretiosum subsp. auranticum | |
2.5.1.54 | Co2+ | 0.1 mM, up to 535% of initial activity | Actinosynnema pretiosum subsp. auranticum | |
2.5.1.54 | Mn2+ | 0.1 mM, up to 550% of initial activity | Actinosynnema pretiosum subsp. auranticum | |
2.5.1.54 | Ni2+ | 0.1 mM, up to 495% of initial activity | Actinosynnema pretiosum subsp. auranticum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.54 | Actinosynnema pretiosum subsp. auranticum | O68903 | - |
- |
2.5.1.54 | Actinosynnema pretiosum subsp. auranticum ATCC 31565 | O68903 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.54 | 0.0127 | - |
pH 7.5, 30°C | Actinosynnema pretiosum subsp. auranticum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.54 | phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Actinosynnema pretiosum subsp. auranticum | 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | - |
? | |
2.5.1.54 | phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Actinosynnema pretiosum subsp. auranticum ATCC 31565 | 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.54 | aroF | - |
Actinosynnema pretiosum subsp. auranticum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.54 | 0.03 | - |
D-erythrose 4-phosphate | pH 7.5, 30°C | Actinosynnema pretiosum subsp. auranticum | |
2.5.1.54 | 0.032 | - |
phosphoenolpyruvate | pH 7.5, 30°C | Actinosynnema pretiosum subsp. auranticum |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.5.1.54 | Actinosynnema pretiosum subsp. auranticum | predicted | - |
5.5 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.54 | 0.069 | - |
D-erythrose 4-phosphate | pH 7.5, 30°C | Actinosynnema pretiosum subsp. auranticum | |
2.5.1.54 | 0.35 | - |
phosphoenolpyruvate | pH 7.5, 30°C | Actinosynnema pretiosum subsp. auranticum |