Literature summary extracted from

de Leon, S.R.; Daniels, K.; Clarke, A.J.
Production and purification of the penicillin-binding protein 3 from Pseudomonas aeruginosa (2010), Protein Expr. Purif., 73, 177-183.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.4.1.129	expressed in Escherichia coli BL21(lambdaDE3)-RIL cells	Pseudomonas aeruginosa

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
2.4.1.129	cytoplasmic membrane	-	Pseudomonas aeruginosa	-	-
2.4.1.129	periplasm	-	Pseudomonas aeruginosa	-	-

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.4.1.129	62200	-	x * 62200, His-tagged enzyme, SDS-PAGE	Pseudomonas aeruginosa
2.4.1.129	62856	-	x * 62856, calculated from amino acid sequence	Pseudomonas aeruginosa

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.4.1.129	Pseudomonas aeruginosa	G3XD46	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.4.1.129	Ni2+-NTA agarose column chromatography	Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.4.1.129	[phenyl-4(n)-3H]benzylpenicillin	-	Pseudomonas aeruginosa	?	-	?

Subunits

EC Number	Subunits	Comment	Organism
2.4.1.129	?	x * 62200, His-tagged enzyme, SDS-PAGE	Pseudomonas aeruginosa
2.4.1.129	?	x * 62856, calculated from amino acid sequence	Pseudomonas aeruginosa

Synonyms

EC Number	Synonyms	Comment	Organism
2.4.1.129	ftsI	-	Pseudomonas aeruginosa
2.4.1.129	PBP 3	-	Pseudomonas aeruginosa
2.4.1.129	penicillin-binding protein 3	-	Pseudomonas aeruginosa

pI Value

EC Number	Organism	Comment	pI Value Maximum	pI Value
2.4.1.129	Pseudomonas aeruginosa	calculated from amino acid sequence	-	9.6

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Release 2023.1 (January 2023)