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Literature summary extracted from
- Discovery and characterization of a thermostable D-lactate dehydrogenase from Lactobacillus jensenii through genome mining (2013), Process Biochem., 48, 109-117.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.28 | D-LDH isozymes D-LDH1, D-LDH2, and D-LDH3, sequence comparisons and phylogenetic analysis | Lactobacillus jensenii |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.28 | 0.04 | - |
NADH | isozyme D-LDH3, pH 8.0, 25°C | Lactobacillus jensenii |  |
| 1.1.1.28 | 0.41 | - |
NAD+ | isozyme D-LDH3, pH 8.0, 25°C | Lactobacillus jensenii | |
| 1.1.1.28 | 0.7 | - |
NADH | isozyme D-LDH1, pH 8.0, 25°C | Lactobacillus jensenii | |
| 1.1.1.28 | 2.58 | - |
NAD+ | isozyme D-LDH2, pH 8.0, 25°C | Lactobacillus jensenii | |
| 1.1.1.28 | 2.75 | - |
NAD+ | isozyme D-LDH1, pH 8.0, 25°C | Lactobacillus jensenii | |
| 1.1.1.28 | 2.83 | - |
NADH | isozyme D-LDH2, pH 8.0, 25°C | Lactobacillus jensenii | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.28 | (R)-lactate + NAD+ | Lactobacillus jensenii | - |
pyruvate + NADH + H+ | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.28 | Lactobacillus jensenii | - |
three isozymes, D-LDH1, D-LDH2, and D-LDH3 | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.28 | 7 | - |
isozyme D-LDH1, pH 8.0, 25°C, substrate D-phenylpyruvate | Lactobacillus jensenii |
| 1.1.1.28 | 9 | - |
isozyme D-LDH2, pH 8.0, 25°C, substrate D-phenylpyruvate | Lactobacillus jensenii |
| 1.1.1.28 | 18 | - |
isozyme D-LDH3, pH 8.0, 25°C, substrate oxaloacetic acid | Lactobacillus jensenii |
| 1.1.1.28 | 28 | - |
isozyme D-LDH3, pH 8.0, 25°C, substrate D-phenylpyruvate | Lactobacillus jensenii |
| 1.1.1.28 | 41 | - |
isozyme D-LDH2, pH 8.0, 25°C, substrate 2-oxoobutyric acid | Lactobacillus jensenii |
| 1.1.1.28 | 41.91 | - |
isozyme D-LDH3, pH 8.0, 25°C, substrate D-lactate | Lactobacillus jensenii |
| 1.1.1.28 | 68 | - |
isozyme D-LDH1, pH 8.0, 25°C, substrate 2-oxoobutyric acid | Lactobacillus jensenii |
| 1.1.1.28 | 88.71 | - |
isozyme D-LDH2, pH 8.0, 25°C, substrate D-lactate | Lactobacillus jensenii |
| 1.1.1.28 | 96.88 | - |
isozyme D-LDH1, pH 8.0, 25°C, substrate D-lactate | Lactobacillus jensenii |
| 1.1.1.28 | 189 | - |
isozyme D-LDH3, pH 8.0, 25°C, substrate 2-oxoobutyric acid | Lactobacillus jensenii |
| 1.1.1.28 | 348 | - |
isozyme D-LDH3, pH 8.0, 25°C, substrate pyruvate | Lactobacillus jensenii |
| 1.1.1.28 | 2877 | - |
isozyme D-LDH2, pH 8.0, 25°C, substrate oxaloacetic acid | Lactobacillus jensenii |
| 1.1.1.28 | 2997 | - |
isozyme D-LDH2, pH 8.0, 25°C, substrate pyruvate | Lactobacillus jensenii |
| 1.1.1.28 | 3151 | - |
isozyme D-LDH1, pH 8.0, 25°C, substrate oxaloacetic acid | Lactobacillus jensenii |
| 1.1.1.28 | 3838 | - |
isozyme D-LDH1, pH 8.0, 25°C, substrate pyruvate | Lactobacillus jensenii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.28 | (R)-lactate + NAD+ | - |
Lactobacillus jensenii | pyruvate + NADH + H+ | - |
r | |
| 1.1.1.28 | (R)-lactate + NAD+ | no activity with L-lactate | Lactobacillus jensenii | pyruvate + NADH + H+ | - |
r | |
| 1.1.1.28 | 2-oxobutyric acid + NADH + H+ | - |
Lactobacillus jensenii | (R)-2-hydroxybutyric acid + NAD+ | - |
ir | |
| 1.1.1.28 | additional information | substrate specificities of the D-LDH isozymes, overview. (R)-2-Hydroxybutyric acid, D-(+)-malic acid, and D-(+)-3-phenyllactic acid are no substrates | Lactobacillus jensenii | ? | - |
? | |
| 1.1.1.28 | oxaloacetic acid + NADH + H+ | - |
Lactobacillus jensenii | (R)-malate + NAD+ | - |
ir | |
| 1.1.1.28 | phenylpyruvate + NADH + H+ | - |
Lactobacillus jensenii | D-phenyllactate + NAD+ | - |
ir | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.28 | D-LDH | - |
Lactobacillus jensenii |
| 1.1.1.28 | D-LDH1 | - |
Lactobacillus jensenii |
| 1.1.1.28 | D-LDH2 | - |
Lactobacillus jensenii |
| 1.1.1.28 | D-LDH3 | - |
Lactobacillus jensenii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.28 | 30 | - |
isozymes D-LDH1 and D-LDH2 | Lactobacillus jensenii |
| 1.1.1.28 | 40 | - |
isozyme D-LDH3 | Lactobacillus jensenii |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.28 | 10 | 60 | isozyme D-LDH3, activity range, profile, overview | Lactobacillus jensenii |
| 1.1.1.28 | 10 | 55 | isozymes D-LDH1 and D-LDH2, activity range, profile, overview | Lactobacillus jensenii |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.28 | 25 | 55 | thermal stability of the D-LDH isozymes, pH 7.0, with sodium pyruvate as a substrate, profiles, overview | Lactobacillus jensenii |
| 1.1.1.28 | 40 | - |
all isozymes, stable up to | Lactobacillus jensenii |
| 1.1.1.28 | 50 | - |
isozyme D-LDH1, inactivation | Lactobacillus jensenii |
| 1.1.1.28 | 55 | - |
isozymes D-LDH2 and D-LDH3, inactivation | Lactobacillus jensenii |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.28 | 0.64 | - |
NAD+ | isozyme D-LDH3, pH 8.0, 25°C | Lactobacillus jensenii | |
| 1.1.1.28 | 3.65 | - |
NAD+ | isozyme D-LDH2, pH 8.0, 25°C | Lactobacillus jensenii | |
| 1.1.1.28 | 4.42 | - |
NAD+ | isozyme D-LDH1, pH 8.0, 25°C | Lactobacillus jensenii | |
| 1.1.1.28 | 8.28 | - |
NADH | isozyme D-LDH3, pH 8.0, 25°C | Lactobacillus jensenii | |
| 1.1.1.28 | 144.4 | - |
NADH | isozyme D-LDH1, pH 8.0, 25°C | Lactobacillus jensenii | |
| 1.1.1.28 | 516.4 | - |
NADH | isozyme D-LDH2, pH 8.0, 25°C | Lactobacillus jensenii | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.28 | 8 | - |
isozymes D-LDH1 and D-LDH2 | Lactobacillus jensenii |
| 1.1.1.28 | 10 | - |
isozyme D-LDH3 | Lactobacillus jensenii |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.28 | 6 | 11 | isozyme D-LDH3, activity range, profile, overview | Lactobacillus jensenii |
| 1.1.1.28 | 7.5 | 11 | isozymes D-LDH1 and D-LDH2, activity range, profile, overview | Lactobacillus jensenii |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.28 | NAD+ | - |
Lactobacillus jensenii | |
| 1.1.1.28 | NADH | - |
Lactobacillus jensenii | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.28 | 1.41 | - |
NAD+ | isozyme D-LDH2, pH 8.0, 25°C | Lactobacillus jensenii | |
| 1.1.1.28 | 1.56 | - |
NAD+ | isozyme D-LDH3, pH 8.0, 25°C | Lactobacillus jensenii | |
| 1.1.1.28 | 1.61 | - |
NAD+ | isozyme D-LDH1, pH 8.0, 25°C | Lactobacillus jensenii | |
| 1.1.1.28 | 182.6 | - |
NADH | isozyme D-LDH2, pH 8.0, 25°C | Lactobacillus jensenii | |
| 1.1.1.28 | 206.3 | - |
NADH | isozyme D-LDH1, pH 8.0, 25°C | Lactobacillus jensenii | |
| 1.1.1.28 | 232.6 | - |
NADH | isozyme D-LDH3, pH 8.0, 25°C | Lactobacillus jensenii | |
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