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Literature summary extracted from

  • Ryu, S.; Kim, J.; Kim, E.; Chung, S.; Lee, S.
    Catalytic reversibility of Pyrococcus horikoshii trehalose synthase: Efficient synthesis of several nucleoside diphosphate glucoses with enzyme recycling (2011), Process Biochem., 46, 128-134.
No PubMed abstract available

Application

EC Number Application Comment Organism
2.4.1.245 synthesis the enzyme may be useful for the regeneration of NDP-alpha-D-glucose from NDP, especially for ADP-alpha-D-glucose from ADP, with trehalose as a glucose resource Pyrococcus horikoshii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.245 2.08
-
GDP-alpha-D-glucose pH 6.0, 60°C Pyrococcus horikoshii
2.4.1.245 2.46
-
UDP-alpha-D-glucose pH 6.0, 60°C Pyrococcus horikoshii
2.4.1.245 3.2
-
ADP-alpha-D-glucose pH 6.0, 60°C Pyrococcus horikoshii
2.4.1.245 14.19
-
ADP pH 6.0, 60°C Pyrococcus horikoshii
2.4.1.245 23.77
-
GDP pH 6.0, 60°C Pyrococcus horikoshii
2.4.1.245 25.24
-
alpha,alpha-trehalose pH 6.0, 60°C Pyrococcus horikoshii
2.4.1.245 29.54
-
UDP pH 6.0, 60°C Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.245 Pyrococcus horikoshii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.245 ADP-alpha-D-glucose + D-glucose GDP-alpha-D-glucose is the most favored in terms of reaction specificity, kcat/Km. UDP-alpha-D-glucose and ADP-alpha-D-glucose are employed with less preferences. The enzyme reversely cleaves alpha,alpha-trehalose to transfer the glucosyl moiety to various NDPs, efficiently producing NDP-alpha-D-glucose. Although ADP-alpha-D-glucose is the least favorable donor, the counterpart, ADP, is the most favorable acceptor for the reverse synthesis of NDP-alpha-D-glucose in kcat/Km. GDP and UDP are less preferred, compared to ADP Pyrococcus horikoshii alpha,alpha-trehalose + ADP
-
r
2.4.1.245 alpha,alpha-trehalose + ADP the enzyme reversely cleaves alpha,alpha-trehalose to transfer the glucosyl moiety to various NDPs, efficiently producing NDP-alpha-D-glucose. Although ADP-alpha-D-glucose is the least favorable donor, the counterpart, ADP, is the most favorable acceptor for the reverse synthesis of NDP-alpha-D-glucose in kcat/Km. GDP and UDP are less preferred, compared to ADP Pyrococcus horikoshii ADP-alpha-D-glucose + D-glucose
-
r
2.4.1.245 alpha,alpha-trehalose + GDP the enzyme reversely cleaves alpha,alpha-trehalose to transfer the glucosyl moiety to various NDPs, efficiently producing NDP-alpha-D-glucose. Although ADP-alpha-D-glucose is the least favorable donor, the counterpart, ADP, is the most favorable acceptor for the reverse synthesis of NDP-alpha-D-glucose in kcat/Km. GDP and UDP are less preferred, compared to ADP Pyrococcus horikoshii GDP-alpha-D-glucose + D-glucose
-
r
2.4.1.245 alpha,alpha-trehalose + UDP the enzyme reversely cleaves alpha,alpha-trehalose to transfer the glucosyl moiety to various NDPs, efficiently producing NDP-alpha-D-glucose. Although ADP-alpha-D-glucose is the least favorable donor, the counterpart, ADP, is the most favorable acceptor for the reverse synthesis of NDP-alpha-D-glucose in kcat/Km. GDP and UDP are less preferred, compared to ADP Pyrococcus horikoshii UDP-alpha-D-glucose + D-glucose
-
r
2.4.1.245 GDP-alpha-D-glucose + D-glucose GDP-alpha-D-glucose is the most favored in terms of reaction specificity, kcat/Km. UDP-alpha-D-glucose and ADP-alpha-D-glucose are employed with less preferences. The enzyme reversely cleaves alpha,alpha-trehalose to transfer the glucosyl moiety to various NDPs, efficiently producing NDP-alpha-D-glucose. Although ADP-alpha-D-glucose is the least favorable donor, the counterpart, ADP, is the most favorable acceptor for the reverse synthesis of NDP-alpha-D-glucose in kcat/Km. GDP and UDP are less preferred, compared to ADP Pyrococcus horikoshii alpha,alpha-trehalose + GDP
-
r
2.4.1.245 UDP-alpha-D-glucose + D-glucose GDP-alpha-D-glucose is the most favored in terms of reaction specificity, kcat/Km. UDP-alpha-D-glucose and ADP-alpha-D-glucose are employed with less preferences. The enzyme reversely cleaves alpha,alpha-trehalose to transfer the glucosyl moiety to various NDPs, efficiently producing NDP-alpha-D-glucose. Although ADP-alpha-D-glucose is the least favorable donor, the counterpart, ADP, is the most favorable acceptor for the reverse synthesis of NDP-alpha-D-glucose in kcat/Km. GDP and UDP are less preferred, compared to ADP Pyrococcus horikoshii alpha,alpha-trehalose + UDP
-
r

Synonyms

EC Number Synonyms Comment Organism
2.4.1.245 TreT
-
Pyrococcus horikoshii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.245 0.44
-
ADP-alpha-D-glucose pH 6.0, 60°C Pyrococcus horikoshii
2.4.1.245 0.53
-
UDP-alpha-D-glucose pH 6.0, 60°C Pyrococcus horikoshii
2.4.1.245 0.54
-
GDP-alpha-D-glucose pH 6.0, 60°C Pyrococcus horikoshii
2.4.1.245 1.2
-
alpha,alpha-trehalose pH 6.0, 60°C Pyrococcus horikoshii
2.4.1.245 1.5
-
UDP pH 6.0, 60°C Pyrococcus horikoshii
2.4.1.245 1.7
-
ADP pH 6.0, 60°C Pyrococcus horikoshii
2.4.1.245 1.7
-
GDP pH 6.0, 60°C Pyrococcus horikoshii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.245 0.05
-
UDP pH 6.0, 60°C Pyrococcus horikoshii
2.4.1.245 0.05
-
alpha,alpha-trehalose pH 6.0, 60°C Pyrococcus horikoshii
2.4.1.245 0.07
-
GDP pH 6.0, 60°C Pyrococcus horikoshii
2.4.1.245 0.12
-
ADP pH 6.0, 60°C Pyrococcus horikoshii
2.4.1.245 0.14
-
ADP-alpha-D-glucose pH 6.0, 60°C Pyrococcus horikoshii
2.4.1.245 0.22
-
UDP-alpha-D-glucose pH 6.0, 60°C Pyrococcus horikoshii
2.4.1.245 0.26
-
GDP-alpha-D-glucose pH 6.0, 60°C Pyrococcus horikoshii