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Literature summary extracted from

  • Xi, J.; Ge, Y.; Kinsland, C.; McLafferty, F.W.; Begley, T.P.
    Biosynthesis of the thiazole moiety of thiamin in Escherichia coli: identification of an acyldisulfide-linked protein--protein conjugate that is functionally analogous to the ubiquitin/E1 complex (2001), Proc. Natl. Acad. Sci. USA, 98, 8513-8518.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.73 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.73 C184S inactive, C184S is equally effective in catalyzing the formation of [ThiS]-COSH as the wild type enzyme. Cys184 is directly involved in the crosslinking of ThiF to ThiS but is not essential for the formation of [ThiS]-COSH Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.73 ATP + [sulfur-carrier protein ThiS] Escherichia coli
-
 diphosphate + adenylyl-[sulfur-carrier protein ThiS]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.73 Escherichia coli P30138
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.73 G-75 Sephadex gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.73 ATP + [sulfur-carrier protein ThiS]
-
 Escherichia coli diphosphate + adenylyl-[sulfur-carrier protein ThiS]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.73 ThiF
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.7.73 ATP
-
 Escherichia coli