BRENDA - Enzyme Database show

Requirement for deoxycytidine kinase in T and B lymphocyte development

Toy, G.; Austin, W.R.; Liao, H.I.; Cheng, D.; Singh, A.; Campbell, D.O.; Ishikawa, T.O.; Lehmann, L.W.; Satyamurthy, N.; Phelps, M.E.; Herschman, H.R.; Czernin, J.; Witte, O.N.; Radu, C.G.; Proc. Natl. Acad. Sci. USA 107, 5551-5556 (2010)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.7.1.74
additional information
deletion of exon 3 inactivates dCK function. Generation of enzyme knockout mice that show 90fold decrease in thymic cellularity compared to wild-type, lymphocyte numbers in the dCK KO mice are 5 to 13fold below normal values
Mus musculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
NTP + deoxycytidine
Mus musculus
-
NDP + dCMP
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Mus musculus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
B-lymphocyte
-
Mus musculus
-
2.7.1.74
bone marrow
-
Mus musculus
-
2.7.1.74
T-lymphocyte
-
Mus musculus
-
2.7.1.74
thymus
-
Mus musculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine
-
723637
Mus musculus
ADP + 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine monophosphate
-
-
-
?
2.7.1.74
NTP + deoxycytidine
-
723637
Mus musculus
NDP + dCMP
-
-
-
?
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.7.1.74
additional information
deletion of exon 3 inactivates dCK function. Generation of enzyme knockout mice that show 90fold decrease in thymic cellularity compared to wild-type, lymphocyte numbers in the dCK KO mice are 5 to 13fold below normal values
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
NTP + deoxycytidine
Mus musculus
-
NDP + dCMP
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
B-lymphocyte
-
Mus musculus
-
2.7.1.74
bone marrow
-
Mus musculus
-
2.7.1.74
T-lymphocyte
-
Mus musculus
-
2.7.1.74
thymus
-
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine
-
723637
Mus musculus
ADP + 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)cytosine monophosphate
-
-
-
?
2.7.1.74
NTP + deoxycytidine
-
723637
Mus musculus
NDP + dCMP
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
2.7.1.74
malfunction
dCK inactivation selectively and profoundly affects T and B cell development, severe impact of dCK inactivation on lymphopoiesis. Inactivation of dCK induces a partial block in B cell development
Mus musculus
2.7.1.74
metabolism
dCK function in the context of cytosolic deoxycytidine metabolism, model, overview
Mus musculus
2.7.1.74
physiological function
deoxycytidine kinase is a rate-limiting enzyme in deoxyribonucleoside salvage, a metabolic pathway that recycles products of DNA degradation. The enzyme phosphorylates and therefore activates nucleoside analogue prodrugs frequently used in cancer, autoimmunity, and viral infections. Normal lymphocyte development critically requires the deoxyribonucleoside salvage pathway
Mus musculus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.7.1.74
malfunction
dCK inactivation selectively and profoundly affects T and B cell development, severe impact of dCK inactivation on lymphopoiesis. Inactivation of dCK induces a partial block in B cell development
Mus musculus
2.7.1.74
metabolism
dCK function in the context of cytosolic deoxycytidine metabolism, model, overview
Mus musculus
2.7.1.74
physiological function
deoxycytidine kinase is a rate-limiting enzyme in deoxyribonucleoside salvage, a metabolic pathway that recycles products of DNA degradation. The enzyme phosphorylates and therefore activates nucleoside analogue prodrugs frequently used in cancer, autoimmunity, and viral infections. Normal lymphocyte development critically requires the deoxyribonucleoside salvage pathway
Mus musculus