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Literature summary extracted from

  • Umitsu, M.; Nishimasu, H.; Noma, A.; Suzuki, T.; Ishitani, R.; Nureki, O.
    Structural basis of AdoMet-dependent aminocarboxypropyl transfer reaction catalyzed by tRNA-wybutosine synthesizing enzyme, TYW2 (2009), Proc. Natl. Acad. Sci. USA, 106, 15616-15621.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.114
-
 Pyrococcus horikoshii
2.5.1.114
-
 Methanocaldococcus jannaschii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.114 crystals of the enzyme-S-adenosyl-L-methionine complex are determined at 2.0 A resolution Methanocaldococcus jannaschii
2.5.1.114 crystals of the enzyme-S-adenosyl-L-methionine complex are grown from the reservoir solution containing 0.1 M Mes-NaOH, pH 6.0, and 14% PEG 10000. The crystals contain one PhTYW2 monomer with one AdoMet or MeSAdo molecule per asymmetric unit, and belong to the space group R3 Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.114 S-adenosyl-L-methionine + 4-demethylwyosine37 in tRNAPhe Pyrococcus horikoshii the enzyme is involved in the biosynthesis of the tricyclic base wybutosine S-methyl-5'-thioadenosine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-demethyl]wyosine37 in tRNAPhe
-
 ?
2.5.1.114 S-adenosyl-L-methionine + 4-demethylwyosine37 in tRNAPhe Methanocaldococcus jannaschii the enzyme is involved in the biosynthesis of the tricyclic base wybutosine S-methyl-5'-thioadenosine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-demethyl]wyosine37 in tRNAPhe
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.114 Methanocaldococcus jannaschii Q58952
-
-
2.5.1.114 Pyrococcus horikoshii O58523
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.114
-
 Pyrococcus horikoshii
2.5.1.114
-
 Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.114 S-adenosyl-L-methionine + 4-demethylwyosine37 in tRNAPhe the enzyme is involved in the biosynthesis of the tricyclic base wybutosine Pyrococcus horikoshii S-methyl-5'-thioadenosine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-demethyl]wyosine37 in tRNAPhe
-
 ?
2.5.1.114 S-adenosyl-L-methionine + 4-demethylwyosine37 in tRNAPhe the enzyme is involved in the biosynthesis of the tricyclic base wybutosine Methanocaldococcus jannaschii S-methyl-5'-thioadenosine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-demethyl]wyosine37 in tRNAPhe
-
 ?
2.5.1.114 S-adenosyl-L-methionine + 4-demethylwyosine37 in tRNAPhe analysis of products by mass spectrometric analysis Pyrococcus horikoshii S-methyl-5'-thioadenosine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-demethyl]wyosine37 in tRNAPhe
-
 ?
2.5.1.114 S-adenosyl-L-methionine + 4-demethylwyosine37 in tRNAPhe analysis of products by mass spectrometric analysis Methanocaldococcus jannaschii S-methyl-5'-thioadenosine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-demethyl]wyosine37 in tRNAPhe
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.114 MJ1557
-
 Methanocaldococcus jannaschii
2.5.1.114 PH0793
-
 Pyrococcus horikoshii
2.5.1.114 TYW2
-
 Pyrococcus horikoshii
2.5.1.114 TYW2
-
 Methanocaldococcus jannaschii

General Information

EC Number General Information Comment Organism
2.5.1.114 physiological function the enzyme is involved in the biosynthesis of the tricyclic base wybutosine Pyrococcus horikoshii
2.5.1.114 physiological function the enzyme is involved in the biosynthesis of the tricyclic base wybutosine Methanocaldococcus jannaschii