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Literature summary extracted from

  • Pojer, F.; Wemakor, E.; Kammerer, B.; Chen, H.; Walsh, C.T.; Li, S.M.; Heide, L.
    CloQ, a prenyltransferase involved in clorobiocin biosynthesis (2003), Proc. Natl. Acad. Sci. USA, 100, 2316-2321.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.111 overexpression in Escherichia coli Streptomyces roseochromogenus subsp. oscitans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.111 0.025
-
 4-hydroxyphenylpyruvate pH 7.5, 30°C Streptomyces roseochromogenus subsp. oscitans
2.5.1.111 0.035
-
 dimethylallyl diphosphate pH 7.5, 30°C Streptomyces roseochromogenus subsp. oscitans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.111 Ca2+ the addition of Ca2+ (2.5 mM) instead of Mg2+ results in 70% of the activity obtained with Mg2+ Streptomyces roseochromogenus subsp. oscitans
2.5.1.111 Mg2+ activates, 2.5 mM is the most effecive concentration. In the absence of divalent cations and in the presence of 5 mM EDTA, the enzyme retains 25% of its original activity. The enzyme is not dependent on the presence of Mg2+ Streptomyces roseochromogenus subsp. oscitans
2.5.1.111 additional information both the zinc and the magnesium contents are below 0.1 mol per mol of CloQ Streptomyces roseochromogenus subsp. oscitans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.111 35600
-
 1 * 35600, SDS-PAGE Streptomyces roseochromogenus subsp. oscitans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.111 dimethylallyl diphosphate + 4-hydroxyphenylpyruvate Streptomyces roseochromogenus subsp. oscitans the enzyme is involved in the biosynthesis of the 3-dimethylallyl-4-hydroxyphenylpyruvate moiety of the aminocoumarin antibiotic clorobiocin diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
 ?
2.5.1.111 dimethylallyl diphosphate + 4-hydroxyphenylpyruvate Streptomyces roseochromogenus subsp. oscitans DS 12.976 the enzyme is involved in the biosynthesis of the 3-dimethylallyl-4-hydroxyphenylpyruvate moiety of the aminocoumarin antibiotic clorobiocin diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.111 Streptomyces roseochromogenus subsp. oscitans Q8GHB2
-
-
2.5.1.111 Streptomyces roseochromogenus subsp. oscitans DS 12.976 Q8GHB2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.111
-
 Streptomyces roseochromogenus subsp. oscitans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.111 dimethylallyl diphosphate + 4-hydroxyphenylpyruvate the enzyme is involved in the biosynthesis of the 3-dimethylallyl-4-hydroxyphenylpyruvate moiety of the aminocoumarin antibiotic clorobiocin Streptomyces roseochromogenus subsp. oscitans diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
 ?
2.5.1.111 dimethylallyl diphosphate + 4-hydroxyphenylpyruvate the enzyme is specific for 4-hydroxyphenylpyruvate. CloQ does not accept prephenic acid, 4-hydroxybenzoate or 4-hydroxybenzaldehyde or as substrate. When dimethylallyl diphosphate is replaced with isopentenyl diphosphate or geranyl diphosphate, no product formation is observed Streptomyces roseochromogenus subsp. oscitans diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
 ?
2.5.1.111 dimethylallyl diphosphate + 4-hydroxyphenylpyruvate the enzyme is involved in the biosynthesis of the 3-dimethylallyl-4-hydroxyphenylpyruvate moiety of the aminocoumarin antibiotic clorobiocin Streptomyces roseochromogenus subsp. oscitans DS 12.976 diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
 ?
2.5.1.111 dimethylallyl diphosphate + 4-hydroxyphenylpyruvate the enzyme is specific for 4-hydroxyphenylpyruvate. CloQ does not accept prephenic acid, 4-hydroxybenzoate or 4-hydroxybenzaldehyde or as substrate. When dimethylallyl diphosphate is replaced with isopentenyl diphosphate or geranyl diphosphate, no product formation is observed Streptomyces roseochromogenus subsp. oscitans DS 12.976 diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.111 monomer 1 * 35600, SDS-PAGE Streptomyces roseochromogenus subsp. oscitans

Synonyms

EC Number Synonyms Comment Organism
2.5.1.111 4HPP dimethylallyltransferase
-
 Streptomyces roseochromogenus subsp. oscitans
2.5.1.111 CloQ
-
 Streptomyces roseochromogenus subsp. oscitans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.111 30
-
 assay at Streptomyces roseochromogenus subsp. oscitans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.111 7.5
-
 assay at Streptomyces roseochromogenus subsp. oscitans

General Information

EC Number General Information Comment Organism
2.5.1.111 physiological function the enzyme is involved in the biosynthesis of the 4-hydroxy-3-dimethylallylphenylpyruvate moiety of the aminocoumarin antibiotic clorobiocin Streptomyces roseochromogenus subsp. oscitans