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Literature summary extracted from

  • Yu, H.; Liu, Q.; Zhang, C.; Lu, M.; Fu, Y.; Im, W.-T.; Jin, F.
    A new ginsenosidase from Aspergillus strain hydrolyzing 20-O-multi-glycoside of PPD ginsenoside (2009), Proc. Biochem., 44, 772-775.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.195 Cu2+
-
 Aspergillus sp.
3.2.1.195 Pb2+
-
 Aspergillus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.195 Ca2+ 50–100 mM slightly enhances the ginsenosidase type II activity Aspergillus sp.
3.2.1.195 Mg2+ 50–100 mM slightly enhances the ginsenosidase type II activity Aspergillus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.195 60000
-
 x * 60000, SDS-PAGE Aspergillus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.195 Aspergillus sp.
-
-
-
3.2.1.195 Aspergillus sp. g48p
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.195
-
 Aspergillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.195 ginsenoside Rb1 + H2O the enzyme hydrolyzes the 20-O-beta-D-(1->6)-glucoside Aspergillus sp. ginsenoside Rd + D-glucose the enzyme produces mainly ginsenoside Rd, and small amount of ginsenoside 20(S)-Rg3 ?
3.2.1.195 ginsenoside Rb1 + H2O the enzyme hydrolyzes the 20-O-beta-D-(1->6)-glucoside Aspergillus sp. g48p ginsenoside Rd + D-glucose the enzyme produces mainly ginsenoside Rd, and small amount of ginsenoside 20(S)-Rg3 ?
3.2.1.195 ginsenoside Rb2 + H2O the enzyme hydrolyzes the 20-O-alpha-(1->6)-arabinopyranoside Aspergillus sp. ginsenoside Rd + L-arabinopyranose the enzyme produces mainly ginsenoside Rd, and small amount of ginsenoside 20(S)-Rg3 ?
3.2.1.195 ginsenoside Rb2 + H2O the enzyme hydrolyzes the 20-O-alpha-(1->6)-arabinopyranoside Aspergillus sp. g48p ginsenoside Rd + L-arabinopyranose the enzyme produces mainly ginsenoside Rd, and small amount of ginsenoside 20(S)-Rg3 ?
3.2.1.195 ginsenoside Rb3 + H2O the enzyme hydrolyzes the 20-O-beta-(1->6)-xyloside Aspergillus sp. ginsenoside Rd + D-xylose the enzyme produces mainly ginsenoside Rd, and small amount of ginsenoside 20(S)-Rg3 ?
3.2.1.195 ginsenoside Rb3 + H2O the enzyme hydrolyzes the 20-O-beta-(1->6)-xyloside Aspergillus sp. g48p ginsenoside Rd + D-xylose the enzyme produces mainly ginsenoside Rd, and small amount of ginsenoside 20(S)-Rg3 ?
3.2.1.195 ginsenoside Rc + H2O the enzyme hydrolyzes the 20-O-alpha-(1->6)-arabinofuranoside Aspergillus sp. ginsenoside Rd + L-arabinofuranose the enzyme produces mainly ginsenoside Rd, and small amount of ginsenoside 20(S)-Rg3 ?
3.2.1.195 ginsenoside Rc + H2O the enzyme hydrolyzes the 20-O-alpha-(1->6)-arabinofuranoside Aspergillus sp. g48p ginsenoside Rd + L-arabinofuranose the enzyme produces mainly ginsenoside Rd, and small amount of ginsenoside 20(S)-Rg3 ?
3.2.1.195 ginsenoside Rd + H2O slowly hydrolyzes the 20-O-glucoside of Rd to produce very small amounts of ginsenoside Rg3 Aspergillus sp. ginsenoside Rg3 + D-glucose
-
 ?
3.2.1.195 additional information ginsenosidase type II does not hydrolyze the 3-O-beta-glucoside of Rb1, Rb2, Rb3. Ginsenosidase type II does not hydrolyze the glycosides of protopanaxatriol type ginsenoside such as Re, Rf, Rg1 Aspergillus sp. ?
-
 ?
3.2.1.195 additional information ginsenosidase type II does not hydrolyze the 3-O-beta-glucoside of Rb1, Rb2, Rb3. Ginsenosidase type II does not hydrolyze the glycosides of protopanaxatriol type ginsenoside such as Re, Rf, Rg1 Aspergillus sp. g48p ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.195 ? x * 60000, SDS-PAGE Aspergillus sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.195 ginsenosidase type II ambiguous Aspergillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.195 40
-
 assay at Aspergillus sp.
3.2.1.195 50
-
-
 Aspergillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.195 5
-
-
 Aspergillus sp.