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Literature summary extracted from

  • Wang, P.; Song, P.; Jin, M.; Zhu, G.
    Isocitrate dehydrogenase from Streptococcus mutans: biochemical properties and evaluation of a putative phosphorylation site at Ser102 (2013), PLoS ONE, 8, e58918.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.41 gene idh, expression of wild-type and mutant enzymes in Escherichia coli glutamate auxotrophic strain Dicd::kanr fused to gene icd, the endogenous icd gene is replaced by the kanamycin resistance gene, the wild-type phenotype of the icd defective strain on minimal medium without glutamate is restored Streptococcus mutans

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.41 S102A site-directed mutagenesis, the mutant shows decreased affinity for isocitrate and 3.3% of wild-type activity Streptococcus mutans
1.1.1.41 S102G site-directed mutagenesis, the mutant shows decreased affinity for isocitrate and 2.8% of wild-type activity Streptococcus mutans
1.1.1.41 S102T site-directed mutagenesis, the mutant shows decreased affinity for isocitrate and 16% of wild-type activity Streptococcus mutans
1.1.1.41 S102Y site-directed mutagenesis, the mutant shows decreased affinity for isocitrate and 1.1% of wild-type activity Streptococcus mutans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.41 Ca2+
-
 Streptococcus mutans
1.1.1.41 Co2+
-
 Streptococcus mutans
1.1.1.41 Cu2+
-
 Streptococcus mutans
1.1.1.41 Ni2+
-
 Streptococcus mutans
1.1.1.41 Zn2+
-
 Streptococcus mutans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.41 0.075
-
 DL-isocitrate pH 7.5, 37°C, recombinant wild-type enzyme Streptococcus mutans
1.1.1.41 0.143
-
 DL-isocitrate pH 7.5, 37°C, recombinant enzyme mutant S102G Streptococcus mutans
1.1.1.41 0.148
-
 DL-isocitrate pH 7.5, 37°C, recombinant enzyme mutant S102A Streptococcus mutans
1.1.1.41 0.15
-
 DL-isocitrate pH 7.5, 37°C, recombinant enzyme mutant S102T Streptococcus mutans
1.1.1.41 0.154
-
 NAD+ pH 7.5, 37°C, recombinant enzyme Streptococcus mutans
1.1.1.41 0.246
-
 NAD+ pH 7.5, 37°C, recombinant enzyme mutant S102A Streptococcus mutans
1.1.1.41 0.295
-
 NAD+ pH 7.5, 37°C, recombinant enzyme mutant S102G Streptococcus mutans
1.1.1.41 0.35
-
 NAD+ pH 7.5, 37°C, recombinant enzyme mutant S102T Streptococcus mutans
1.1.1.41 0.385
-
 DL-isocitrate pH 7.5, 37°C, recombinant enzyme mutant S102Y Streptococcus mutans
1.1.1.41 1.56
-
 NAD+ pH 7.5, 37°C, recombinant enzyme mutant S102Y Streptococcus mutans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.41 Mn2+ best divalent cation Streptococcus mutans
1.1.1.41 additional information the enzyme is dependent on divalent cations Streptococcus mutans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.41 70000
-
 recombinant enzyme, gel filtration Streptococcus mutans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.41 DL-isocitrate + NAD+ Streptococcus mutans
-
 2-oxoglutarate + CO2 + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.41 Streptococcus mutans
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.1.41 phosphoprotein catalytically important phosphorylation site at Ser102 Streptococcus mutans

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.41 recombinant wild-type and mutant enzymes from Escherichia coli Streptococcus mutans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.41 DL-isocitrate + NAD+
-
 Streptococcus mutans 2-oxoglutarate + CO2 + NADH
-
 r
1.1.1.41 additional information the enzyme is also active with NADP+ and NADPH, cf. EC 1.1.1.42 Streptococcus mutans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.41 homodimer
-
 Streptococcus mutans

Synonyms

EC Number Synonyms Comment Organism
1.1.1.41 IDH
-
 Streptococcus mutans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.41 45
-
-
 Streptococcus mutans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.41 25 55 activity range, profile overview Streptococcus mutans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.41 40
-
 purified recombinant enzyme, stable up to, rapid inactivation above Streptococcus mutans
1.1.1.41 50
-
 purified recombinant enzyme, inactivation Streptococcus mutans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.41 1.5
-
 DL-isocitrate pH 7.5, 37°C, recombinant enzyme mutant S102Y Streptococcus mutans
1.1.1.41 3
-
 NAD+ pH 7.5, 37°C, recombinant enzyme mutant S102A Streptococcus mutans
1.1.1.41 3.3
-
 DL-isocitrate pH 7.5, 37°C, recombinant enzyme mutant S102A Streptococcus mutans
1.1.1.41 3.5
-
 NAD+ pH 7.5, 37°C, recombinant enzyme mutant S102Y Streptococcus mutans
1.1.1.41 4
-
 DL-isocitrate pH 7.5, 37°C, recombinant enzyme mutant S102G Streptococcus mutans
1.1.1.41 9.1
-
 NAD+ pH 7.5, 37°C, recombinant enzyme mutant S102G Streptococcus mutans
1.1.1.41 25
-
 DL-isocitrate pH 7.5, 37°C, recombinant enzyme mutant S102T Streptococcus mutans
1.1.1.41 56
-
 NAD+ pH 7.5, 37°C, recombinant wild-type enzyme Streptococcus mutans
1.1.1.41 59
-
 NAD+ pH 7.5, 37°C, recombinant enzyme mutant S102T Streptococcus mutans
1.1.1.41 124
-
 DL-isocitrate pH 7.5, 37°C, recombinant wild-type enzyme Streptococcus mutans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.41 7.8
-
-
 Streptococcus mutans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.41 7.2 9.2 activity range, profile overview Streptococcus mutans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.41 additional information the enzyme is also active with NADP+ and NADPH, cf. EC 1.1.1.42 Streptococcus mutans
1.1.1.41 NAD+
-
 Streptococcus mutans
1.1.1.41 NADH
-
 Streptococcus mutans

General Information

EC Number General Information Comment Organism
1.1.1.41 evolution the ancient NAD-dependent IDHs might be the underlying origin of phosphorylation mechanism used by their bacterial NADP-dependent homologues Streptococcus mutans
1.1.1.41 additional information Ser102 plays an important role in substrate binding and is required for the enzyme function Streptococcus mutans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.41 0.000002
-
 NAD+ pH 7.5, 37°C, recombinant enzyme mutant S102Y Streptococcus mutans
1.1.1.41 0.000004
-
 DL-isocitrate pH 7.5, 37°C, recombinant enzyme mutant S102Y Streptococcus mutans
1.1.1.41 0.00001
-
 NAD+ pH 7.5, 37°C, recombinant enzyme mutant S102A Streptococcus mutans
1.1.1.41 0.000022
-
 DL-isocitrate pH 7.5, 37°C, recombinant enzyme mutant S102A Streptococcus mutans
1.1.1.41 0.000028
-
 DL-isocitrate pH 7.5, 37°C, recombinant enzyme mutant S102G Streptococcus mutans
1.1.1.41 0.00003
-
 NAD+ pH 7.5, 37°C, recombinant enzyme mutant S102G Streptococcus mutans
1.1.1.41 0.00017
-
 NAD+ pH 7.5, 37°C, recombinant enzyme mutant S102T Streptococcus mutans
1.1.1.41 0.00017
-
 DL-isocitrate pH 7.5, 37°C, recombinant enzyme mutant S102T Streptococcus mutans
1.1.1.41 0.00036
-
 NAD+ pH 7.5, 37°C, recombinant enzyme Streptococcus mutans
1.1.1.41 0.00165
-
 DL-isocitrate pH 7.5, 37°C, recombinant wild-type enzyme Streptococcus mutans