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Literature summary extracted from

  • Orsomando, G.; Cialabrini, L.; Amici, A.; Mazzola, F.; Ruggieri, S.; Conforti, L.; Janeckova, L.; Coleman, M.P.; Magni, G.
    Simultaneous single-sample determination of NMNAT isozyme activities in mouse tissues (2012), PLoS ONE, 7, e53271.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.1 expressed in Escherichia coli BL21(DE3) cells Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.1 0.0252
-
nicotinamide ribonucleotide isoform NMNAT1, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
2.7.7.1 0.0335
-
ATP isoform NMNAT1, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
2.7.7.1 0.0385
-
nicotinamide ribonucleotide isoform NMNAT2, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
2.7.7.1 0.039
-
ATP isoform NMNAT3, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
2.7.7.1 0.082
-
ATP isoform NMNAT2, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
2.7.7.1 0.1176
-
nicotinamide ribonucleotide isoform NMNAT3, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.1 Co2+ isoform NMNAT3 is predominantly activated by Co2+ ions (5 mM) Mus musculus
2.7.7.1 Mg2+ isoform NMNAT2 is predominantly activated by Mg2+ ions (0.5 mM) Mus musculus
2.7.7.1 Zn2+ isoform NMNAT1 is predominantly activated by Zn2+ ions (0.05 mM) Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.1 Mus musculus Q5HZI3 isoform NMNAT2
-
2.7.7.1 Mus musculus Q99JR6 isoform NMNAT3
-
2.7.7.1 Mus musculus Q9EPA7 isoform NMNAT1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.1 Ni-NTA affinity chromatography Mus musculus
2.7.7.1 Talon affinity column chromatography Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.1 brain
-
Mus musculus
-
2.7.7.1 liver
-
Mus musculus
-

Storage Stability

EC Number Storage Stability Organism
2.7.7.1 -20°C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 20% loss of activity Mus musculus
2.7.7.1 -20°C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 55% loss of activity Mus musculus
2.7.7.1 -20°C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 60% loss of activity Mus musculus
2.7.7.1 4°C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 55% loss of activity Mus musculus
2.7.7.1 4°C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 60% loss of activity Mus musculus
2.7.7.1 4°C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 90% loss of activity Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.1 ATP + nicotinamide ribonucleotide
-
Mus musculus diphosphate + NAD+
-
r
2.7.7.1 ATP + nicotinic acid mononucleotide
-
Mus musculus diphosphate + deamido-NAD+
-
r
2.7.7.1 GTP + nicotinamide ribonucleotide
-
Mus musculus ?
-
?
2.7.7.1 ITP + nicotinamide ribonucleotide
-
Mus musculus ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.1 nicotinamide mononucleotide adenylyltransferase
-
Mus musculus
2.7.7.1 NMNAT1 isoform Mus musculus
2.7.7.1 NMNAT2 isoform Mus musculus
2.7.7.1 NMNAT3 isoform Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.1 0.74
-
ATP isoform NMNAT3, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
2.7.7.1 0.75
-
nicotinamide ribonucleotide isoform NMNAT3, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
2.7.7.1 2.33
-
ATP isoform NMNAT2, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
2.7.7.1 2.87
-
nicotinamide ribonucleotide isoform NMNAT2, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
2.7.7.1 15
-
ATP isoform NMNAT1, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
2.7.7.1 15.5
-
nicotinamide ribonucleotide isoform NMNAT1, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.7.1 10
-
nicotinamide ribonucleotide isoform NMNAT3, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
2.7.7.1 20
-
ATP isoform NMNAT3, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
2.7.7.1 30
-
ATP isoform NMNAT2, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
2.7.7.1 70
-
nicotinamide ribonucleotide isoform NMNAT2, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
2.7.7.1 450
-
ATP isoform NMNAT1, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus
2.7.7.1 610
-
nicotinamide ribonucleotide isoform NMNAT1, in 30 mM HEPES/KOH, pH 7.5, at 37°C Mus musculus