Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gandhi, A.K.; Desai, J.V.; Ghatge, M.S.; di Salvo, M.L.; Di Biase, S.; Danso-Danquah, R.; Musayev, F.N.; Contestabile, R.; Schirch, V.; Safo, M.K.
    Crystal structures of human pyridoxal kinase in complex with the neurotoxins, ginkgotoxin and theophylline: insights into pyridoxal kinase inhibition (2012), PLoS ONE, 7, e40954.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.35 in complex with ginkgotoxin and theophylline, hanging drop vapor diffusion method, using 48-50% (w/v) 2-methyl-1,3 propanediol as precipitant, at 22°C Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.35 Caffeine 21% inhibition at 0.1 mM Homo sapiens
2.7.1.35 enprofylline 33% inhibition at 0.1 mM Homo sapiens
2.7.1.35 ginkgotoxin i.e. 4'-O-methylpyridoxine, complete inhibition at 0.1 mM Homo sapiens
2.7.1.35 lamotrigine 45% inhibition at 0.1 mM Homo sapiens
2.7.1.35 Theobromine 22% inhibition at 0.1 mM Homo sapiens
2.7.1.35 theophylline 60% inhibition at 0.1 mM Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.35 Mg2+ required Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.35 Homo sapiens O00764
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.35 ATP + pyridoxal
-
 Homo sapiens ADP + pyridoxal 5'-phosphate
-
 ir
2.7.1.35 ATP + pyridoxamine
-
 Homo sapiens ADP + pyridoxamine 5'-phosphate
-
 ir

Synonyms

EC Number Synonyms Comment Organism
2.7.1.35 PL kinase
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.35 0.003
-
 ginkgotoxin in 20 mM sodium BES buffer, pH 7.2, at 37°C Homo sapiens
2.7.1.35 0.05
-
 theophylline in 20 mM sodium BES buffer, pH 7.2, at 37°C Homo sapiens
2.7.1.35 0.056
-
 lamotrigine in 20 mM sodium BES buffer, pH 7.2, at 37°C Homo sapiens
2.7.1.35 0.228
-
 enprofylline in 20 mM sodium BES buffer, pH 7.2, at 37°C Homo sapiens