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Literature summary extracted from

  • Okada, K.; Angkawidjaja, C.; Koga, Y.; Takano, K.; Kanaya, S.
    Characteristic features of kynurenine aminotransferase allosterically regulated by (alpha)-ketoglutarate in cooperation with kynurenine (2012), PLoS ONE, 7, e40307.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.6.1.7 L-kynurenine allosterically regulated Pyrococcus horikoshii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.7 His-tagged protein expressed in Escherichia coli BL21-CodonPlus (DE3) Pyrococcus horikoshii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.7 sitting-drop vapor diffusion method Pyrococcus horikoshii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.7 2-oxoglutarate inhibited by high concentrations, allosterically regulated Pyrococcus horikoshii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.7 0.00173
-
 2-oxobutyrate 0.1 mM L-kynurenine, pH 7.5, 25°C Pyrococcus horikoshii
2.6.1.7 0.00241
-
 2-oxo-4-methylthiobutyrate 0.1 mM L-kynurenine, pH 7.5, 25°C Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.7 48000
-
 2 * 48000, SDS-PAGE, crystal structure Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.7 Pyrococcus horikoshii
-
-
-
2.6.1.7 Pyrococcus horikoshii OT-3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.7 immobilized metal ion affinity chromatography (Ni2+) Pyrococcus horikoshii

Storage Stability

EC Number Storage Stability Organism
2.6.1.7 full activity after 12 month at -80°C, 50 mM HEPES/NaOH, pH 7.5, 100 mM NaCl Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.7 L-kynurenine + 2-oxo-4-methylthiobutyrate
-
 Pyrococcus horikoshii kynurenate + L-methionine
-
 ?
2.6.1.7 L-kynurenine + 2-oxo-4-methylthiobutyrate
-
 Pyrococcus horikoshii OT-3 kynurenate + L-methionine
-
 ?
2.6.1.7 L-kynurenine + 2-oxobutyrate
-
 Pyrococcus horikoshii kynurenate + ?
-
 ?
2.6.1.7 L-kynurenine + 2-oxobutyrate
-
 Pyrococcus horikoshii OT-3 kynurenate + ?
-
 ?
2.6.1.7 L-kynurenine + 2-oxoglutarate
-
 Pyrococcus horikoshii kynurenate + L-glutamate + H2O
-
 ?
2.6.1.7 L-kynurenine + 2-oxoglutarate
-
 Pyrococcus horikoshii OT-3 kynurenate + L-glutamate + H2O
-
 ?
2.6.1.7 L-kynurenine + oxaloacetate
-
 Pyrococcus horikoshii kynurenate + L-aspartate + H2O
-
 ?
2.6.1.7 L-kynurenine + oxaloacetate
-
 Pyrococcus horikoshii OT-3 kynurenate + L-aspartate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.7 homodimer 2 * 48000, SDS-PAGE, crystal structure Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
2.6.1.7 kynurenine aminotransferase
-
 Pyrococcus horikoshii

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.7 pyridoxal 5'-phosphate
-
 Pyrococcus horikoshii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.7 0.02011
-
 2-oxoglutarate pH 7.5, 25°C Pyrococcus horikoshii