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Literature summary extracted from

  • Peng, R.H.; Tian, Y.S.; Xiong, A.S.; Zhao, W.; Fu, X.Y.; Han, H.J.; Chen, C.; Jin, X.F.; Yao, Q.H.
    A novel 5-enolpyruvylshikimate-3-phosphate synthase from Rahnella aquatilis with significantly reduced glyphosate sensitivity (2012), PLoS ONE, 7, e39579.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.5.1.19 agriculture transgenic Arabidopsis thaliana plants expressing the enzyme from Rahnella aquatilis are more resistant to glyphosate exposure than plants expressing the Escherichia coli enzyme Rahnella aquatilis

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.19 G96A mutant with improved resistance towards glyphosate Rahnella aquatilis
2.5.1.19 T38K/R40V/R222Q/S224V/I225V/Q226K mutant with the c-strand of subdomain 3 and the f-strand of subdomain 5 substituted by the corresponding region of the Escherichia coli enzyme. The mutant enzyme exhibits greater sensitivity to glyphosate than the wild type with little change of affinity for substrates shikimate-3-phosphate and phosphoenolpyruvate Rahnella aquatilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.19 glyphosate
-
 Rahnella aquatilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.19 0.078
-
 phosphoenolpyruvate wild-type, pH 7.0, 28°C Rahnella aquatilis
2.5.1.19 0.084
-
 3-phosphoshikimate mutant G96A, pH 7.0, 28°C Rahnella aquatilis
2.5.1.19 0.086
-
 3-phosphoshikimate wild-type, pH 7.0, 28°C Rahnella aquatilis
2.5.1.19 0.148
-
 phosphoenolpyruvate mutant G96A, pH 7.0, 28°C Rahnella aquatilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.19 47000
-
 x * 47000, SDS-PAGE Rahnella aquatilis

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.19 Rahnella aquatilis C9EGX2
-
-
2.5.1.19 Rahnella aquatilis GR20 C9EGX2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Rahnella aquatilis phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 ?
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Rahnella aquatilis GR20 phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.19 ? x * 47000, SDS-PAGE Rahnella aquatilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.19 0.049
-
 glyphosate wild-type, pH 7.0, 28°C Rahnella aquatilis
2.5.1.19 0.45
-
 glyphosate mutant G96A, pH 7.0, 28°C Rahnella aquatilis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.5.1.19 0.26
-
 wild-type, pH 7.0, 28°C Rahnella aquatilis glyphosate
2.5.1.19 0.34
-
 mutant G96A, pH 7.0, 28°C Rahnella aquatilis glyphosate

General Information

EC Number General Information Comment Organism
2.5.1.19 physiological function enzyme is able to restore the growth of the Escherichia coli mutant ER2799, with the 5-enolpyruvylshikimate-3-phosphate synthase gene deleted from its genome, on an M9 minimal medium containing 60 mM glyphosate Rahnella aquatilis