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Literature summary extracted from

  • Cortes-Romero, C.; Martinez-Hernandez, A.; Mellado-Mojica, E.; Lopez, M.G.; Simpson, J.
    Molecular and functional characterization of novel fructosyltransferases and invertases from Agave tequilana (2012), PLoS ONE, 7, e35878.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.243 genes Atq6G-FFT-1 and -2, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain X-33 Agave tequilana
3.2.1.26 expressed in Pichia pastoris Agave tequilana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.26 cell wall
-
 Agave tequilana 5618
-
3.2.1.26 vacuole
-
 Agave tequilana 5773
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.243 69700
-
 x * 70700, about, Atq6G-FFT-1, sequence calculation, x * 69700, about, Atq6G-FFT-2, sequence calculation Agave tequilana
2.4.1.243 70700
-
 x * 70700, about, Atq6G-FFT-1, sequence calculation, x * 69700, about, Atq6G-FFT-2, sequence calculation Agave tequilana
3.2.1.26 64200
-
 x * 64200, isoform Cwinv-1, calculated from amino acid sequence Agave tequilana
3.2.1.26 70400
-
 x * 70400, isoform Vinv-1, calculated from amino acid sequence Agave tequilana

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.243 Agave tequilana
-
 genes Atq6G-FFT-1 and -2
-
2.4.1.243 Agave tequilana azul
-
 genes Atq6G-FFT-1 and -2
-
3.2.1.26 Agave tequilana I1ZBQ8 isoform Cwinv-1 isoform; variant Azul
-
3.2.1.26 Agave tequilana I1ZBQ9 isoform Vinv-1 isoform; variant Azul
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.243 glycoprotein Atq6G-FFT-1 and Atq6G-FFT-2 contains each seven glycosylation sites Agave tequilana
3.2.1.26 glycoprotein contains 3 glycosylation sites Agave tequilana
3.2.1.26 glycoprotein contains 4 glycosylation sites Agave tequilana

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.26 Ni-NTA affinity resin column chromatography Agave tequilana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.243 additional information age-related expression of Atq6G-FFT-1 in 5 and 7 year old plants, overview Agave tequilana
-
3.2.1.26 leaf
-
 Agave tequilana
-
3.2.1.26 mature plant
-
 Agave tequilana
-
3.2.1.26 stem
-
 Agave tequilana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.243 1-kestose
-
 Agave tequilana ?
-
 ?
2.4.1.243 1-kestose
-
 Agave tequilana azul ?
-
 ?
2.4.1.243 1-kestose + 3 sucrose
-
 Agave tequilana 3 neokestose + H2O plus a 4c tetrasaccharide moiety ?
2.4.1.243 1-kestose + 3 sucrose
-
 Agave tequilana azul 3 neokestose + H2O plus a 4c tetrasaccharide moiety ?
2.4.1.243 additional information the Atq6GFFT-1 consensus sequence is DPCG, Atq6GFFT-1 and Atq6G-1FFT-2 utilize primarily 1-kestose as a substrate, a Asn/Trp couple is involved in substrate recognition Agave tequilana ?
-
 ?
2.4.1.243 additional information the Atq6GFFT-1 consensus sequence is DPCG, Atq6GFFT-1 and Atq6G-1FFT-2 utilize primarily 1-kestose as a substrate, a Asn/Trp couple is involved in substrate recognition Agave tequilana azul ?
-
 ?
2.4.1.243 sucrose
-
 Agave tequilana neokestose
-
 ?
2.4.1.243 sucrose
-
 Agave tequilana azul neokestose
-
 ?
3.2.1.26 sucrose + H2O
-
 Agave tequilana D-fructose + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.243 ? x * 70700, about, Atq6G-FFT-1, sequence calculation, x * 69700, about, Atq6G-FFT-2, sequence calculation Agave tequilana
2.4.1.243 More Atq6G-FFT-1 and Atq6G-FFT-2 sequence comparisons Agave tequilana
3.2.1.26 ? x * 64200, isoform Cwinv-1, calculated from amino acid sequence Agave tequilana
3.2.1.26 ? x * 70400, isoform Vinv-1, calculated from amino acid sequence Agave tequilana

Synonyms

EC Number Synonyms Comment Organism
2.4.1.243 6G-FFT
-
 Agave tequilana
2.4.1.243 Atq6G-FFT-1
-
 Agave tequilana
2.4.1.243 Atq6G-FFT-2
-
 Agave tequilana
2.4.1.243 fructan: fructan 6G-fructosyltransferase
-
 Agave tequilana
3.2.1.26 cell wall invertase
-
 Agave tequilana
3.2.1.26 Cwinv-1 isoform Agave tequilana
3.2.1.26 Vacuolar invertase
-
 Agave tequilana
3.2.1.26 Vinv-1 isoform Agave tequilana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.243 30
-
 assay at Agave tequilana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.243 Agave tequilana Atq6G-FFT-2, sequence calculation
-
 5.11
2.4.1.243 Agave tequilana Atq6G-FFT-1, sequence calculation
-
 5.57
3.2.1.26 Agave tequilana isoform Vinv-1, calculated from amino acid sequence
-
 5.4
3.2.1.26 Agave tequilana isoform Cwinv-1, calculated from amino acid sequence
-
 8.4

General Information

EC Number General Information Comment Organism
2.4.1.243 metabolism the enzyme is required for fructan biosynthesis Agave tequilana