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Literature summary extracted from

  • Schmidt, H.; Mesters, J.; Wu, J.; Woodard, R.; Hilgenfeld, R.; Mamat, U.
    Evidence for a two-metal-ion mechanism in the cytidyltransferase KdsB, an enzyme involved in lipopolysaccharide biosynthesis (2011), PLoS ONE, 6, e23231.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.38 to 2.1 A resolution. Two Mg2+ ions are part of the active site of the enzyme Aquifex aeolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.38 0.16
-
 CTP pH 9.0, 50°C Aquifex aeolicus
2.7.7.38 0.452
-
 3-deoxy-D-manno-octulosonate pH 9.0, 50°C Aquifex aeolicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.38 Mg2+ two Mg2+ ions are part of the active site of the enzyme Aquifex aeolicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.38 27000
-
 2 * 27000, SDS-PAGE Aquifex aeolicus
2.7.7.38 53000
-
 gel filtration Aquifex aeolicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.38 Aquifex aeolicus O66914
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate
-
 Aquifex aeolicus diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
 ?
2.7.7.38 additional information no substrates: ATP, GTP Aquifex aeolicus ?
-
 ?
2.7.7.38 UTP + 3-deoxy-D-manno-octulosonate about 45% of the activity with CTP Aquifex aeolicus diphosphate + UMP-3-deoxy-D-manno-octulosonate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.38 dimer 2 * 27000, SDS-PAGE Aquifex aeolicus

Synonyms

EC Number Synonyms Comment Organism
2.7.7.38 CKS
-
 Aquifex aeolicus
2.7.7.38 CMP-KDO synthetase
-
 Aquifex aeolicus
2.7.7.38 KdsB
-
 Aquifex aeolicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.38 90
-
-
 Aquifex aeolicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.38 30 95
-
 Aquifex aeolicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.38 9.5
-
 3-deoxy-D-manno-octulosonate pH 9.0, 50°C Aquifex aeolicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.38 9
-
-
 Aquifex aeolicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.38 8.5 9.5
-
 Aquifex aeolicus