BRENDA - Enzyme Database

Nicotinamide phosphoribosyltransferase/visfatin does not catalyze nicotinamide mononucleotide formation in blood plasma

Hara, N.; Yamada, K.; Shibata, T.; Osago, H.; Tsuchiya, M.; PLoS ONE 6, e22781 (2011)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.2.12
ATP
essential activator
Homo sapiens
Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
2.4.2.12
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.12
0.00051
-
5-phosphoribosyl 1-diphosphate
with 1 mM ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.00122
-
nicotinamide
with 1 mM ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.00806
-
nicotinamide
without ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0232
-
5-phosphoribosyl 1-diphosphate
without ATP, pH 7.5, 37°C
Homo sapiens
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.4.2.12
Homo sapiens
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.12
0.00213
-
at 0.001 mM 5-phosphoribosyl 1-diphosphate, without ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0259
-
at 0.050 mM 5-phosphoribosyl 1-diphosphate, without ATP, pH 7.5, 37°C; substrate 5-phosphoribosyl 1-diphosphate, without ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0393
-
substrate nicotinamide, without ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0397
-
substrate nicotinamide, with 1 mM ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0481
-
at 0.050 mM 5-phosphoribosyl 1-diphosphate, with 1 mM ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0704
-
at 0.001 mM 5-phosphoribosyl 1-diphosphate, with 1 mM ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0741
-
substrate 5-phosphoribosyl 1-diphosphate, with 1 mM ATP, pH 7.5, 37°C
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.4.2.12
nicotinamide + 5-phosphoribosyl 1-diphosphate
-
723535
Homo sapiens
nicotinamide mononucleotide + diphosphate
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
2.4.2.12
nicotinamide phosphoribosyltransferase
-
Homo sapiens
2.4.2.12
nicotinamide phosphoribosyltransferase/Visfatin
does not catalyze nicotinamide mononucleotide formation in blood plasma
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.2.12
ATP
essential activator
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.12
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.12
0.00051
-
5-phosphoribosyl 1-diphosphate
with 1 mM ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.00122
-
nicotinamide
with 1 mM ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.00806
-
nicotinamide
without ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0232
-
5-phosphoribosyl 1-diphosphate
without ATP, pH 7.5, 37°C
Homo sapiens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.12
0.00213
-
at 0.001 mM 5-phosphoribosyl 1-diphosphate, without ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0259
-
at 0.050 mM 5-phosphoribosyl 1-diphosphate, without ATP, pH 7.5, 37°C; substrate 5-phosphoribosyl 1-diphosphate, without ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0393
-
substrate nicotinamide, without ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0397
-
substrate nicotinamide, with 1 mM ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0481
-
at 0.050 mM 5-phosphoribosyl 1-diphosphate, with 1 mM ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0704
-
at 0.001 mM 5-phosphoribosyl 1-diphosphate, with 1 mM ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0741
-
substrate 5-phosphoribosyl 1-diphosphate, with 1 mM ATP, pH 7.5, 37°C
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.4.2.12
nicotinamide + 5-phosphoribosyl 1-diphosphate
-
723535
Homo sapiens
nicotinamide mononucleotide + diphosphate
-
-
-
?