BRENDA - Enzyme Database show

Nicotinamide phosphoribosyltransferase/visfatin does not catalyze nicotinamide mononucleotide formation in blood plasma

Hara, N.; Yamada, K.; Shibata, T.; Osago, H.; Tsuchiya, M.; PLoS ONE 6, e22781 (2011)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.2.12
ATP
essential activator
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
2.4.2.12
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.12
0.00051
-
5-phosphoribosyl 1-diphosphate
with 1 mM ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.00122
-
nicotinamide
with 1 mM ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.00806
-
nicotinamide
without ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0232
-
5-phosphoribosyl 1-diphosphate
without ATP, pH 7.5, 37°C
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.12
Homo sapiens
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.12
0.00213
-
at 0.001 mM 5-phosphoribosyl 1-diphosphate, without ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0259
-
at 0.050 mM 5-phosphoribosyl 1-diphosphate, without ATP, pH 7.5, 37°C; substrate 5-phosphoribosyl 1-diphosphate, without ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0393
-
substrate nicotinamide, without ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0397
-
substrate nicotinamide, with 1 mM ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0481
-
at 0.050 mM 5-phosphoribosyl 1-diphosphate, with 1 mM ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0704
-
at 0.001 mM 5-phosphoribosyl 1-diphosphate, with 1 mM ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0741
-
substrate 5-phosphoribosyl 1-diphosphate, with 1 mM ATP, pH 7.5, 37°C
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.12
nicotinamide + 5-phosphoribosyl 1-diphosphate
-
723535
Homo sapiens
nicotinamide mononucleotide + diphosphate
-
-
-
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.2.12
ATP
essential activator
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.12
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.12
0.00051
-
5-phosphoribosyl 1-diphosphate
with 1 mM ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.00122
-
nicotinamide
with 1 mM ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.00806
-
nicotinamide
without ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0232
-
5-phosphoribosyl 1-diphosphate
without ATP, pH 7.5, 37°C
Homo sapiens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.12
0.00213
-
at 0.001 mM 5-phosphoribosyl 1-diphosphate, without ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0259
-
at 0.050 mM 5-phosphoribosyl 1-diphosphate, without ATP, pH 7.5, 37°C; substrate 5-phosphoribosyl 1-diphosphate, without ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0393
-
substrate nicotinamide, without ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0397
-
substrate nicotinamide, with 1 mM ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0481
-
at 0.050 mM 5-phosphoribosyl 1-diphosphate, with 1 mM ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0704
-
at 0.001 mM 5-phosphoribosyl 1-diphosphate, with 1 mM ATP, pH 7.5, 37°C
Homo sapiens
2.4.2.12
0.0741
-
substrate 5-phosphoribosyl 1-diphosphate, with 1 mM ATP, pH 7.5, 37°C
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.12
nicotinamide + 5-phosphoribosyl 1-diphosphate
-
723535
Homo sapiens
nicotinamide mononucleotide + diphosphate
-
-
-
?