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Literature summary extracted from

  • Kuettel, S.; Greenwald, J.; Kostrewa, D.; Ahmed, S.; Scapozza, L.; Perozzo, R.
    Crystal structures of T. b. rhodesiense adenosine kinase complexed with inhibitor and activator: implications for catalysis and hyperactivation (2011), PLoS Negl. Trop. Dis., 5, e1164.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.20 3-(4-ethylphenyl)-5-(tetrahydro-2H-pyran-4-yl)-1H-pyrazole
-
Trypanosoma brucei
2.7.1.20 3-(4-phenoxyphenyl)-5-(tetrahydro-2H-pyran-4-yl)-1H-pyrazole
-
Trypanosoma brucei
2.7.1.20 4-[3-(4-chlorophenyl)-1H-pyrazol-5-yl]morpholine
-
Trypanosoma brucei
2.7.1.20 4-[5-(4-phenoxyphenyl)-2H-pyrazol-3-yl]morpholine best activator Trypanosoma brucei
2.7.1.20 4-[[3-(4-phenoxyphenyl)-1H-pyrazol-5-yl]methyl]morpholine
-
Trypanosoma brucei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.20
-
Trypanosoma brucei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.20 in complex with the bisubstrate inhibitor P1,P5-di(adenosine-5')-pentaphosphate (at 16°C, in 0.2 M sodium acetate trihydrate, 0.1 M sodium cacodylate, pH 6.0, and 24% (w/v) polyethylene glycol 8000 (crystal form 1), or in 0.1 M tri-sodium citrate dihydrate, pH 5.6, 20% (v/v) isopropanol, and 20% (w/v) polytethylene glycol 4000 (crystal form 2)) and with the activator 4-[5-(4-phenoxyphenyl)-2H-pyrazol-3-yl]morpholine (at 4°C in 0.1 M Tris, pH 9.0 and 60% (v/v) 2-methyl-2,4-pentanediol), sitting drop vapor diffusion method Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.20 P1,P5-di(adenosine-5')pentaphosphate
-
Trypanosoma brucei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.20 Mg2+ required Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.20 Trypanosoma brucei Q584S0 subsp. rhodesiense
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.20
-
Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.20 ATP + adenosine ATP is the preferred phosphoryl donor Trypanosoma brucei ADP + AMP
-
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Synonyms

EC Number Synonyms Comment Organism
2.7.1.20 ATP:adenosine 5'-phosphotransferase
-
Trypanosoma brucei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.20 43
-
the melting temperature of the apo-enzyme is at 43°C Trypanosoma brucei

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.20 0.0294
-
pH and temperature not specified in the publication Trypanosoma brucei P1,P5-di(adenosine-5')-pentaphosphate