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Literature summary extracted from

  • Rousvoal, S.; Groisillier, A.; Dittami, S.; Michel, G.; Boyen, C.; Tonon, T.
    Mannitol-1-phosphate dehydrogenase activity in Ectocarpus siliculosus, a key role for mannitol synthesis in brown algae (2011), Planta, 233, 261-273.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.17 gene EsM1PDH2 is located at Esi0020_0181, DNA and amino acid sequence determination and analysis, sequence comparison, real-time-PCR expression analysis, expression in Escherichia coli strain BL21(DE3) Ectocarpus siliculosus
1.1.1.17 gene EsM1PDH3 is located at Esi0080_0017, DNA and amino acid sequence determination and analysis, sequence comparison, real-time-PCR expression analysis, expression in Escherichia coli strain BL21(DE3) Ectocarpus siliculosus
1.1.1.17 gene EsM1PHD1 is located at Esi0017_0062, DNA and amino acid sequence determination and analysis, sequence comparison, real-time-PCR expression analysis, expression in Escherichia coli strain BL21(DE3) Ectocarpus siliculosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.17 4-hydroxymercuribenzoate strong inhibition; strong inhibition; strong inhibition Ectocarpus siliculosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.17 NaCl activates Ectocarpus siliculosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.17 47000
-
 x * 47000, isozyme EsM1PHD3 Ectocarpus siliculosus
1.1.1.17 58000
-
 x * 58000, isozyme EsM1PHD1 Ectocarpus siliculosus
1.1.1.17 60000
-
 x * 60000, isozyme EsM1PHD2 Ectocarpus siliculosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.17 D-mannitol 1-phosphate + NAD+ Ectocarpus siliculosus the enzyme shows higher activity in the reduction reaction D-fructose 6-phosphate + NADH + H+
-
 r
1.1.1.17 D-mannitol 1-phosphate + NAD+ Ectocarpus siliculosus Ec32 / CCAP 1310/4 the enzyme shows higher activity in the reduction reaction D-fructose 6-phosphate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.17 Ectocarpus siliculosus D7FMJ8 predicted; gene EsM1PDH2
-
1.1.1.17 Ectocarpus siliculosus D7G793 partial, predicted; gene EsM1PDH3
-
1.1.1.17 Ectocarpus siliculosus D8LI09 predicted; gene EsM1PHD1
-
1.1.1.17 Ectocarpus siliculosus Ec32 / CCAP 1310/4 D7FMJ8 predicted; gene EsM1PDH2
-
1.1.1.17 Ectocarpus siliculosus Ec32 / CCAP 1310/4 D7G793 partial, predicted; gene EsM1PDH3
-
1.1.1.17 Ectocarpus siliculosus Ec32 / CCAP 1310/4 D8LI09 predicted; gene EsM1PHD1
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.17 0.002
-
 algal extract, pH 6.3, 30°C, in presence of NaCl Ectocarpus siliculosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.17 D-mannitol 1-phosphate + NAD+
-
 Ectocarpus siliculosus D-fructose 6-phosphate + NADH + H+
-
 r
1.1.1.17 D-mannitol 1-phosphate + NAD+ the enzyme shows higher activity in the reduction reaction Ectocarpus siliculosus D-fructose 6-phosphate + NADH + H+
-
 r
1.1.1.17 D-mannitol 1-phosphate + NAD+
-
 Ectocarpus siliculosus Ec32 / CCAP 1310/4 D-fructose 6-phosphate + NADH + H+
-
 r
1.1.1.17 D-mannitol 1-phosphate + NAD+ the enzyme shows higher activity in the reduction reaction Ectocarpus siliculosus Ec32 / CCAP 1310/4 D-fructose 6-phosphate + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.17 ? x * 47000, isozyme EsM1PHD3 Ectocarpus siliculosus
1.1.1.17 ? x * 58000, isozyme EsM1PHD1 Ectocarpus siliculosus
1.1.1.17 ? x * 60000, isozyme EsM1PHD2 Ectocarpus siliculosus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.17 M1PDH
-
 Ectocarpus siliculosus
1.1.1.17 M1PDH1
-
 Ectocarpus siliculosus
1.1.1.17 M1PDH2
-
 Ectocarpus siliculosus
1.1.1.17 M1PDH3
-
 Ectocarpus siliculosus
1.1.1.17 mannitol-1-phosphate dehydrogenase
-
 Ectocarpus siliculosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.17 30
-
 assay at Ectocarpus siliculosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.17 6.3
-
 reduction reaction Ectocarpus siliculosus
1.1.1.17 9
-
 oxidation reaction Ectocarpus siliculosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.17 NAD+
-
 Ectocarpus siliculosus
1.1.1.17 NADH
-
 Ectocarpus siliculosus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.17 Ectocarpus siliculosus isozyme EsM1PHD3
-
 5.19
1.1.1.17 Ectocarpus siliculosus isozyme EsM1PHD1
-
 5.93
1.1.1.17 Ectocarpus siliculosus isozyme EsM1PHD2
-
 6.94

General Information

EC Number General Information Comment Organism
1.1.1.17 metabolism the enzyme catalyzes the first step of the synthesis of mannitol, i.e. reduction of the photo-assimilate fructose-6-phosphate, mannitol cycle, overview Ectocarpus siliculosus